压力性尿失禁大鼠泌尿生殖道组织及耻尾肌NO、NPY的表达及意义

发布时间：2018-05-06 08:48

本文选题：压力性尿失禁 + NO　；参考：《山西医科大学》2014年硕士论文

【摘要】：目的：1．建立压力性尿失禁大鼠模型，观察SUI模型大鼠和对照大鼠的泌尿生殖道组织和耻尾肌的组织形态学差异。2.检测SUI大鼠和对照组盆底组织中神经递质NO、NPY的表达情况，探究其与SUI发生的关系。方法：1.40只SD大鼠，，随机分为对照组和实验组。对照组常规饲养，不作任何处理。实验组采用模拟难产，常规饲养一周后切除双侧卵巢。四周后所有大鼠行尿流动力学检测最大膀胱容量和漏尿点压力，并行喷嚏实验。取建模成功的15只大鼠作为实验组。取同样条件喂养下的正常大鼠20只作为对照组。2.处死所有大鼠，取尿道、阴道前壁及耻尾肌，行HE染色，观察上述标本的组织形态学差异。3.行免疫组化染色方法检测上述标本中N0与NPY的表达，积分光密度值比较采用独立样本t检验。结果：1.对照组和实验组大鼠最大膀胱容量分别为（1.95±0.13）ml和（1.22±0.15）ml。漏尿点压力分别为（27.18±4.90）mmHg和（13.63±4.22）mmHg。实验组最大膀胱容量和漏尿点压力均小于对照组，差异有统计学意义(P0.01)。喷嚏实验中，SUI组全为阳性，对照组均为阴性。2. HE染色：对照组可见尿道、阴道前壁、耻尾肌染色均匀，结构完整并紧密相连，肌纤维排列整齐；实验组可见染色不均匀,组织萎缩变薄,肌纤维走形紊乱、断裂。3.免疫组化染色：对照组大鼠尿道、阴道前壁、耻尾肌一氧化氮(NO)的积分光密度值分别为：（18.49±2.54）；（17.95±1.37）；（14.78±2.62）。实验组一氧化氮(NO)的积分光密度值分别为：（8.17±0.88）；（8.04±0.73）；（7.19±1.08）。对照组神经肽Y的积分光密度值分别为：（14.04±1.68）；（15.18±2.47）；（13.19±1.72）。实验组神经肽Y的积分光密度值分别为：（6.83±0.58）；（7.27±0.91）；（7.37±0.94）。与对照组相比，实验组大鼠盆底组织中NO与NPY表达的积分光密度值降低，差异有统计学意义(P0.01)。结论：l. SUI大鼠盆底肌肉萎缩变薄、肌纤维断裂使盆底支持力量减弱可能与SUI发生有关。2. NO和NPY在压力性尿失禁大鼠的尿道、阴道前壁和耻尾肌组织中表达减少，提示其可能参与了SUI的发生和发展。
[Abstract]:Purpose 1. A rat model of stress urinary incontinence was established to observe the histomorphologic differences of genitourinary tissues and pubic caudal muscles between SUI model rats and control rats. The expression of neurotransmitter No-NPY in pelvic floor tissues of SUI rats and control group was detected to explore the relationship between NON-NPY and the pathogenesis of SUI. Methods 1.40 Sprague-Dawley rats were randomly divided into control group and experimental group. The control group was fed regularly without any treatment. The experimental group was fed with analogue dystocia for one week and both ovaries were resected. Four weeks later, the maximal bladder volume and leak point pressure were measured by urodynamics in all rats, and sneezing test was performed. Fifteen rats were selected as experimental group. 20 normal rats fed under the same conditions were used as control group. 2. 2. All the rats were killed, the urethra, vagina anterior wall and pubic tail muscle were taken, and HE staining was used to observe the histomorphologic difference of the above specimens. 3. The expression of N0 and NPY in the above specimens was detected by immunohistochemical staining. The integral optical density was compared by independent sample t test. The result is 1: 1. The maximal bladder volume of control group and experimental group were 1.95 卤0.13)ml and 1.22 卤0.15 ml, respectively. The pressure of leak point was 27.18 卤4.90)mmHg and 13.63 卤4.22mm Hg, respectively. The maximum bladder volume and leak point pressure in the experimental group were lower than those in the control group, and the difference was statistically significant (P 0.01). In the sneezing test, the SUI group was all positive, and the control group was negative. 2. HE staining: in the control group, the urethra, the anterior wall of vagina, the pubic and caudal muscles were stained evenly, the structure was complete and closely connected, and the muscle fibers were arranged neatly. In the experimental group, the staining was uneven, the tissue atrophy became thinner, the muscle fiber was disorganized, and the muscle fiber was broken. 3. Immunohistochemical staining: the integral optical density of nitric oxide (no) in urethra, vagina anterior wall and pubic caudal muscle of rats in control group was 18.49 卤2.54, 17.95 卤1.37 and 14.78 卤2.62, respectively. The integral optical density of nitric oxide (no) in the experimental group was 8.17 卤0.88, 8.04 卤0.73, 7.19 卤1.08, respectively. The integral optical density of neuropeptide Y in the control group was 14.04 卤1.68, 15.18 卤2.47, 13.19 卤1.72, respectively. The integral optical density of neuropeptide Y in the experimental group was 6.83 卤0.58, 7.27 卤0.91 and 7.37 卤0.94, respectively. Compared with the control group, the integral optical density of no and NPY expression in the pelvic floor of the experimental group was significantly lower than that in the control group (P 0.01). Conclusion: 1. In SUI rats, the pelvic floor muscle atrophy became thinner and the pelvic floor support strength decreased due to the breakage of muscle fiber, which may be related to the occurrence of SUI. The decreased expression of no and NPY in urethra, anterior vaginal wall and pubic muscle of rats with stress urinary incontinence suggests that no and NPY may be involved in the occurrence and development of SUI.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R694.54

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