伊红Y水试验法应用研究进展

发布时间：2018-05-12 18:43

本文选题：伊红Y水试验 + 精子膜　；参考：《中华男科学杂志》2015年06期

【摘要】：基于体外活体染色技术、低渗肿胀试验和水试验原理建立的伊红Y水试验方法可同步检测精子头部和尾部、精子膜结构和功能的完整性,在中国已被临床广泛应用。伊红Y水试验方法学上的3个特点包括:1同时对精子头部和尾部进行检测,全面评估精子膜各部位损伤的内在联系,便于观察;2用蒸馏水代替常用的配方溶液,避免不同渗透压或不同比例糖和电解质溶液对精子膜有不同作用影响水分子通过精子膜,既简化了方法,又使反应标准化;3检测时间短,可以重复检测,适合治疗前后反复检测。本文综述伊红Y水试验法的基本方法及其改进,在精子功能检查、男性不育常规精液分析、睾丸穿刺精子质量评估、精液冷冻保存程序研究、受微波辐射男性精子膜完整性等相关研究方面的应用。
[Abstract]:Based on in vitro staining technique, the eosin Y water test method based on the principle of hypoosmotic swelling test and water test can simultaneously detect the integrity of sperm head and tail, and the structure and function of sperm membrane. It has been widely used in clinical practice in China. The three characteristics of eosin Y water test methodology include the detection of the head and tail of spermatozoa at the same time by 1: 1, the comprehensive evaluation of the internal relationship between the damage of the various parts of the sperm membrane, and the convenience of observing the use of distilled water to replace the commonly used formula solution. To avoid different osmotic pressure or different proportion of sugar and electrolyte solution have different effects on sperm membrane, water molecules pass through sperm membrane, which not only simplifies the method, but also makes the reaction standardized detection time short, and can be detected repeatedly. Suitable for repeated detection before and after treatment. This paper reviews the basic method and its improvement of eosin Y water test, studies on sperm function test, routine semen analysis of male infertility, testicular puncture sperm quality evaluation, semen cryopreservation procedure, Application of microwave irradiation in the study of the integrity of sperm membrane.
【作者单位】：江苏省计划生育科学技术研究所;
【基金】：江苏省科技厅科技基础设施建设计划(BM2013058)~~
【分类号】：R698.2

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