雄激素对雌激素引发去势雄性SD大鼠前列腺炎症及炎症因子的抑制作用

发布时间：2018-05-13 08:46

本文选题：前列腺炎 + 雌激素　；参考：《中国药理学与毒理学杂志》2017年06期

【摘要】：目的给予不同比例苯甲酸雌二醇(E2)和丙酸睾酮(T)诱导SD大鼠前列腺炎,探讨炎症因子肿瘤坏死因子α(TNF-α)、环氧合酶2(COX-2)和巨噬细胞炎症蛋白1α(MIP~(-1)α)表达的变化。方法 SD大鼠无菌条件下去势后,颈背部分别sc给予E20.25 mg·kg~(-1)+T 0.25,0.5和1.0 mg·kg~(-1),连续给药30 d。取血离心取血清,采用ELISA检测E2,T和双氢睾酮(DHT)激素水平。HE染色观察前列腺组织病理变化,免疫组化检测前列腺中TNF-α,COX-2和MIP~(-1)α的表达。结果 ELISA检测结果显示,与假手术组比较,E20.25 mg·kg~(-1)组血清E2水平明显升高[(80±7)ng·L~(-1),P0.01];E20.25 mg·kg~(-1)组和E2+T 0.25 mg·kg~(-1)组血清T水平显著下降[111±6和(111±5)nmol·L~(-1),P0.05]。与去势对照组相比,E20.25 mg·kg~(-1)组血清E2明显升高[(80±7)ng·L~(-1),P0.01],其他各指标未见统计学差异。光学显微镜观察发现,假手术组和去势对照组前列腺组织结构完整、腺腔和间充质无炎症细胞浸润;E20.25 mg·kg~(-1)组前列腺腺腔内有少量炎症细胞浸润,间充质未见炎症细胞浸润;E2+T 3个剂量组间充质内有少量炎症细胞浸润。假手术组前列腺上皮细胞及前列腺基质细胞胞浆内TNF-α,COX-2和MIP~(-1)α均为阴性表达或极少量表达,去势对照组和E20.25 mg·kg~(-1)组TNF-α,COX-2和MIP~(-1)α均为强阳性表达,E2+T 3个剂量组TNF-α,COX-2和MIP~(-1)α呈弱阳性表达。结论雄激素具有抑制雌激素引起的去势SD大鼠前列腺炎及炎症因子TNF-α,COX-2和MIP~(-1)α表达的作用。
[Abstract]:Objective to investigate the expression of inflammatory factor TNF- 伪 (TNF- 伪), cyclooxygenase-2 (COX-2) and macrophage inflammatory protein 1 伪 (1 伪) in SD rats with prostatitis induced by estradiol benzoate (E _ 2) and testosterone propionate (T _ (2). Methods Sprague-Dawley rats were castrated under aseptic conditions. Sc was given E 20.25 mg 路kg ~ (-1) T 0.25m 0.5 and 1.0 mg / kg ~ (-1) respectively for 30 days. Serum samples were collected from blood centrifugation. The levels of E2T and DHT were detected by ELISA. The pathological changes of prostate tissue were observed by HE staining. The expressions of TNF- 伪 -COX-2 and MIP- 1) 伪 in prostate were detected by immunohistochemistry. Results compared with sham-operated group, serum E 2 level in E20.25 mg / kg ~ (-1) group was significantly higher than that in sham operation group [80 卤7)ng ~ (-1)] E _ (20.25 mg 路kg ~ (-1) group and E _ (2) T _ (0.25 mg / kg ~ (-1) group [111 卤6 and 111 卤5)nmol ~ (-1)] group (P < 0.05). Compared with the ovariectomized control group, the serum E _ 2 in the E _ (20.25 mg 路kg ~ (-1) group was significantly higher than that in the ovariectomized control group (P < 0.01), but there was no statistical difference among the other indexes. The results of optical microscope showed that the prostate tissue structure was intact in sham operation group and castrated control group, and there were a few inflammatory cells infiltrated in prostatic gland cavity and mesenchymal cells in group E 20.25 mg kg 路kg ~ (-1). No infiltration of inflammatory cells was found in mesenchymal cells. The expression of TNF- 伪 COX-2 and MIPn-1) 伪 in the cytoplasm of prostatic epithelial cells and stromal cells in sham operation group was negative or very small, while TNF- 伪 -coCOX-2 and MIPn-1) 伪 were strongly positive in castrated control group and E20.25 mg / kg ~ (-1) group. TNF- 伪 COX-2 and MIPn-1) 伪 were weakly expressed in three dose groups of E _ 2 T and E _ (20.25 mg / kg ~ (-1). Conclusion androgen can inhibit the expression of prostatitis and inflammatory cytokines TNF- 伪 COX-2 and MIPn-1) 伪 in ovariectomized SD rats.
【作者单位】：上海市计划生育科学研究所药理毒理学研究室中国生育调节药物毒理学检测中心;国家人口和计划生育委员会计划生育药具重点实验室;
【基金】：上海市研发平台专项(15DZ2290400) 上海市“科技创新行动计划”实验动物研究项目(14140901302)~~
【分类号】：R-332;R697.3

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