基于免疫炎症介导机制探讨清肾颗粒抗肾脏纤维化的研究

发布时间：2018-05-19 13:53

本文选题：单个核细胞核转录因子κBp65 + 白介素-17　；参考：《安徽中医药大学》2014年硕士论文

【摘要】：目的：观察慢性肾衰竭（chronic renal failure,CRF）湿热证患者血清白介素-17（interleukin17,IL-17）、外周血单个核细胞（peripheral blood mononuclearcells,PBMC）核转录因子-κBp65(nuclear factors-κB,NF-κBp65）、血清Ⅲ型胶原（collagen type Ⅲ,Col-Ⅲ）的变化情况及清肾颗粒的干预作用。方法：根据随机数字表将68例CRF湿热证患者随机分为治疗组和对照组各34例，并选取30例健康体检者作为正常组。过程中失访7例（因加服金水宝剔除者3例，加服百令胶囊剔除者4例），其中治疗组4例，对照组3例，实际完成61例，治疗组30例，对照组31例。治疗组男18例，女12例，年龄平均（53.20±9.41）岁，对照组男19例，女12例，年龄平均（49.06±12.53）岁。治疗组与对照组均给予西医基础治疗及中药保留灌肠，治疗组加用清肾颗粒，每日3次，每次1袋，疗程8周。检测治疗组与对照组治疗前后血肌酐（serum creatinine,Scr）、IL-17、Col-Ⅲ及PBMC中NF-κBp65水平，估算肾小球滤过率（estimatedglomerular filtration rate,eGFR），并观察两组患者治疗前后中医症候积分变化情况。正常组检测血清IL-17、Col-Ⅲ及外周血PBMC中NF-κBp65的含量。结果：1.临床疾病疗效比较：治疗组疾病疗效总有效率为86.67%，对照组为58.06%，两组比较差异有统计学意义（P0.05）。2.中医证候疗效比较：治疗组中医证候疗效总有效率为86.67%，对照组为45.16%，两组比较有显著性差异（P0.01）。3.中医证候积分比较：治疗前治疗组中医证候积分与对照组比较，差异无统计学意义（P0.05），治疗组治疗后中医证候积分显著低于治疗前（P0.01），对照组治疗后中医证候积分与治疗前比较，差异无统计学意义（P0.05），治疗组治疗后中医证候积分显著低于同期对照组（P0.01）。4.Scr、eGFR比较：（1）治疗前治疗组与对照组Scr水平的差异无统计学意义（P0.05），治疗组治疗后Scr水平较治疗前明显降低（P0.01），对照组治疗后Scr水平与治疗前比较，差异无统计学意义（P0.05），治疗组治疗后Scr水平低于同期对照组（P0.05）。（2）治疗前治疗组与对照组eGFR水平的差异无统计学意义（P0.05），治疗组治疗后eGFR水平较治疗前明显升高（P0.01），对照组治疗后eGFR水平与治疗前比较，差异无统计学意义（P0.05），治疗组治疗后eGFR水平高于同期对照组（P0.05）。5.血清IL-17比较：治疗组治疗前血清IL-17水平较正常组高，差异有显著性（P0.01），对照组治疗前血清IL-17水平较正常组高，差异有显著性（P0.01）。但治疗组和对照组治疗前血清IL-17水平比较，差异无统计学意义（P0.05）。治疗组治疗后血清IL-17水平较治疗前降低（P0.05），对照组治疗后血清IL-17水平与治疗前相比较，差异无统计学意义（P0.05），治疗组治疗后血清IL-17水平低于同期对照组（P0.05）。6.PBMC中NF-κBp65比较：治疗组治疗前PBMC中NF-κBp65含量较正常组高，差异有显著性（P0.01），对照组治疗前PBMC中NF-κBp65含量较正常组高，差异有显著性（P0.01）。但治疗组和对照组治疗前PBMC中NF-κBp65含量比较，差异无统计学意义（P0.05）。治疗组治疗后PBMC中NF-κBp65含量较治疗前明显降低（P0.01），对照组治疗后PBMC中NF-κBp65含量与治疗前相比较，差异无统计学意义（P0.05），治疗组治疗后PBMC中NF-κBp65含量显著低于同期对照组（P0.01）。7.血清Col-Ⅲ比较：治疗组、对照组治疗前血清Col-Ⅲ水平均较正常组高，差异有显著性（P0.01），治疗组和对照组治疗前血清Col-Ⅲ水平比较，差异无统计学意义（P0.05）。治疗组治疗后血清Col-Ⅲ水平较治疗前明显降低（P0.01），对照组治疗后血清Col-Ⅲ水平与治疗前相比较，差异无统计学意义（P0.05），治疗组治疗后血清Col-Ⅲ水平显著低于同期对照组（P0.01）。结论：1.清肾颗粒可改善CRF湿热证患者临床症状、降低Scr、升高eGFR。2.CRF湿热证患者血清IL-17、Col-Ⅲ及PBMC中NF-κBp65水平均升高。3.清肾颗粒可以降低CRF湿热证患者血清IL-17、Col-Ⅲ及PBMC中NF-κBp65水平，说明清肾颗粒在防治RF中有较好的作用。4.治疗后安全性指标检测无异常变化，过程中未出现药物不良反应，说明清肾颗粒安全性良好。目的：通过检测单侧输尿管梗阻（unilateral ureteral obstruction,UUO）所致肾间质纤维化（renal interstitial fibrosis,RIF）大鼠外周血中辅助性T淋巴细胞（CD4+T细胞）与抑制性T淋巴细胞（CD8+T细胞）比值、Th17细胞比例及肾脏组织中白介素-17(interleukin-17,IL-17)、核转录因子κB-p65(nuclear factors-κBp65,NF-κBp65)、Ⅲ型胶原(collagen type Ⅲ,Col-Ⅲ)的表达，观察UUO大鼠是否存在免疫功能的紊乱、炎症介质的高释放及清肾颗粒的干预作用。方法：取60只健康雄性SD大鼠，按随机区组法随机分为5组：清肾颗粒组、百令胶囊组、模型组、假手术组、正常组，每组12只。各组大鼠实验前检测血尿素氮（blood urea nitrogen,BUN）、血肌酐（serum creatinine,Scr），结果差异无统计学意义（P0.05）。清肾颗粒组、百令胶囊组及模型组均以UUO法制备肾间质纤维化大鼠模型，假手术组以前三组同样的手术路径分离输尿管但不结扎。清肾颗粒组以0.6g/100g的清肾颗粒溶于4ml温水中灌胃，百令胶囊组以0.03g/100g的百令胶囊溶于4ml温水中灌胃，模型组、假手术组及正常组均以4ml温水灌胃。各组大鼠每日灌胃一次，疗程为3周。实验大鼠灌胃结束后腹主动脉取血并取左侧肾脏，术前留取各组大鼠24小时尿量。生化法检测BUN、Scr、24小时尿蛋白定量。流式细胞仪技术检测外周血CD4+/CD8+、Th17细胞比例。HE染色和Masson染色行肾脏病理检查。免疫组化法检测肾组织中IL-17、Col-Ⅲ、NF-κBp65的表达情况。结果：1.BUN、Scr比较：（1）治疗前各组大鼠BUN之间比较，无显著性差异（P0.05）。治疗后，清肾颗粒组、百令胶囊组、模型组大鼠BUN均升高，与正常组及假手术组相比差异均有显著性（P0.01）。假手术组大鼠BUN与正常组大鼠比较，差异无统计学意义（P0.05）。清肾颗粒组和百令胶囊组大鼠BUN均较模型组低，差异有显著性（P0.01）。清肾颗粒组大鼠BUN较百令胶囊组低，差异有统计学意义（P0.05）。（2）治疗前各组大鼠Scr之间比较，无显著性差异（P0.05）。治疗后，清肾颗粒组、百令胶囊组、模型组大鼠Scr均升高，与正常组及假手术组相比差异均有显著性（P0.01）。假手术组大鼠Scr与正常组大鼠比较，差异（P0.05）。清肾颗粒组和百令胶囊组大鼠Scr均较模型组低，差异有显著性（P0.01）。清肾颗粒组大鼠Scr较百令胶囊组低，差异有统计学意义（P0.05）。2.24小时尿蛋白定量比较：百令胶囊组、模型组大鼠24小时尿蛋白定量均较正常组及假手术组高，差异有显著性（P0.01）。清肾颗粒组大鼠24小时尿蛋白定量较正常组高，差异有显著性（P0.01），清肾颗粒组大鼠24小时尿蛋白定量较假手术组高，差异有统计学意义（P0.05）。假手术组大鼠24小时尿蛋白定量与正常组比较，差异无统计学意义（P0.05）。清肾颗粒组、百令胶囊组大鼠24小时尿蛋白定量均较模型组低，，差异有显著性（P0.01）。清肾颗粒组大鼠24小时尿蛋白定量较百令胶囊组低，差异有统计学意义（P0.05）。3.外周血CD4+/CD8+比较：清肾颗粒组大鼠外周血CD4+/CD8+较正常组及假手术组高，差异有统计学意义（P0.05）。百令胶囊组、模型组大鼠外周血CD4+/CD8+均较正常组及假手术组高，差异有显著性（P0.01）。假手术组大鼠外周血CD4+/CD8+与正常组比较，差异无统计学意义（P0.05）。清肾颗粒组外周血CD4+/CD8+较模型组低，差异有显著性（P0.01）。百令胶囊组外周血CD4+/CD8+较模型组低，差异有统计学意义（P0.05）。清肾颗粒组大鼠外周血CD4+/CD8+较百令胶囊组低，差异有显著性（P0.01）。4.外周血Th17细胞比例比较：清肾颗粒组、百令胶囊组、模型组大鼠外周Th17细胞比例均较正常组及假手术组高，差异有显著性（P0.01）。假手术组大鼠外周Th17细胞比例与正常组比较，差异无统计学意义（P0.05）。清肾颗粒组大鼠外周Th17细胞比例较模型组低，差异有显著性（P0.01）。百令胶囊组鼠外周Th17细胞比例较模型组低，差异有统计学意义（P0.05）。而清肾颗粒组大鼠外周Th17细胞比例较百令胶囊组低，差异有统计学意义（P0.05）。5.免疫组化IL-17染色比较：清肾颗粒组、百令胶囊组、模型组大鼠IL-17半定量评分均较正常组及假手术组高，差异有显著性（P0.01）。假手术组大鼠IL-17半定量评分与正常组比较，差异无统计学意义（P0.05）。清肾颗粒组、百令胶囊组大鼠IL-17半定量评分均较模型组低，差异有显著性（P0.01）。而清肾颗粒组大鼠IL-17半定量评分较百令胶囊组低，差异有统计学意义（P0.05）。6.免疫组化NF-κBp65染色比较：清肾颗粒组、百令胶囊组、模型组大鼠NF-κBp65着色半定量评分均较正常组及假手术组高，差异有显著性（P0.01）。假手术组大鼠NF-κBp65着色半定量评分与正常组比较，差异无统计学意义（P0.05）。清肾颗粒组、百令胶囊组大鼠NF-κBp65着色半定量评分均较模型组低，差异有显著性（P0.01）。而清肾颗粒组大鼠NF-κBp65着色半定量评分较百令胶囊组低，差异有统计学意义（P0.05）。7.免疫组化Col-Ⅲ染色比较：百令胶囊组、模型组大鼠Col-Ⅲ着色半定量评分均较正常组及假手术组高，差异有显著性（P0.01），清肾颗粒组大鼠Col-Ⅲ着色半定量评分较正常组及假手术组高，差异有统计学意义（P0.05）。假手术组大鼠Col-Ⅲ着色半定量评分与正常组比较，差异无统计学意义（P0.05）。清肾颗粒组、百令胶囊组大鼠Col-Ⅲ着色半定量评分均较模型组低，差异有显著性（P0.01）。而清肾颗粒组大鼠Col-Ⅲ着色半定量评分较百令胶囊组低，差异有统计学意义（P0.05）。结论：1.免疫炎症介导机制参与肾间质纤维化的发生。2.清肾颗粒可降低肾纤维化大鼠血肌酐、尿素氮，减少24h尿蛋白定量。3.清肾颗粒可减轻肾纤维化大鼠肾脏病理损害，减轻炎症反应。4.清肾颗粒可通过调节免疫功能紊乱，减少炎症介质IL-17的释放，抑制NF-κB信号通路，减少NF-κBp65的释放，下调肾纤维化大鼠肾间质Col-Ⅲ的表达，减少肾间质细胞外基质（extracellular matrix,ECM）的沉积，从而起到拮抗肾脏纤维化（renal fibrosis,RF）的作用。
[Abstract]:Objective: To observe the changes of serum interleukin -17 (interleukin17, IL-17), nuclear factor kappa Bp65 (peripheral blood mononuclearcells, PBMC) in peripheral blood mononuclear cells (peripheral blood mononuclearcells, PBMC) and serum type III collagen (peripheral blood mononuclearcells, PBMC) in patients with chronic renal failure (chronic renal failure, CRF). The intervention of particles.
Methods: 68 cases of CRF damp heat syndrome were randomly divided into treatment group and 34 cases in control group, and 30 cases of health examination were selected as normal group. 7 cases were lost in the process (3 cases with Jinshui Po culling and 4 cases of capsule culling), 4 cases in the treatment group and 3 cases in the control group, 30 cases were completed and 30 cases in the treatment group were completed. There were 31 cases in the group. The treatment group was 18 men and 12 women, the average age (53.20 + 9.41) years, the control group of 19 men and 12 women, the average age (49.06 + 12.53) years old. The treatment group and the control group were given western medicine basic treatment and traditional Chinese medicine retention enema, the treatment group was added with the Qing Shen Granule, 3 times a day, 1 bags, course 8 weeks. Test the treatment group and the control group blood before and after treatment. The level of NF- kappa Bp65 in serum creatinine, Scr, IL-17, Col- III and PBMC was used to estimate the glomerular filtration rate (estimatedglomerular filtration rate, eGFR). The changes of TCM syndrome score in the two groups were observed before and after treatment.
Results: 1. comparison of the curative effect of clinical disease: the total effective efficiency of the treatment group was 86.67%, the control group was 58.06%, the two groups were statistically significant (P0.05).2. TCM syndrome curative effect comparison: the total effective rate of TCM syndrome in the treatment group was 86.67%, the control group was 45.16%, the two groups had significant difference (P0.01).3. TCM syndrome score Comparison: there was no significant difference between the TCM syndrome score of the treatment group and the control group (P0.05). The TCM syndrome score of the treatment group was significantly lower than that before the treatment (P0.01). The score of TCM syndrome was not statistically significant (P0.05) after the treatment in the control group, and the TCM syndrome score of the treatment group was significantly lower than that of the same group after treatment. Compared with the control group (P0.01).4.Scr and eGFR: (1) there was no significant difference in the level of Scr between the treatment group and the control group (P0.05). The Scr level in the treatment group was significantly lower than that before the treatment (P0.01). The level of Scr in the control group was not statistically significant (P0.05) after treatment (P0.05). The Scr level of the treatment group was lower than that of the same period after treatment. Group (P0.05). (2) there was no significant difference in the level of eGFR between the treatment group and the control group (P0.05). The level of eGFR in the treatment group was significantly higher than that before the treatment (P0.01). The difference of eGFR level in the control group was not statistically significant (P0.05) after treatment (P0.05). The level of eGFR in the treatment group was higher than that of the control group (P0.05).5. serum IL-17. Comparison: the level of serum IL-17 in the treatment group was higher than that of the normal group before treatment (P0.01). The serum IL-17 level in the control group was higher than that of the normal group before treatment (P0.01), but there was no significant difference between the treatment group and the control group before the treatment (P0.05). The serum IL-17 level in the treatment group was compared with that of the treatment group before treatment. The level of serum IL-17 in the control group was lower than that in the control group (P0.05). The difference was not statistically significant (P0.05). The level of serum IL-17 in the treatment group was lower than that in the control group (P0.05).6.PBMC NF- kappa Bp65: the NF- kappa Bp65 content in the treatment group was higher than that of the normal group before the treatment group, and the difference was significant (P0.01), and the control group before treatment. The content of NF- kappa Bp65 was higher than that of the normal group (P0.01), but there was no significant difference in the content of NF- kappa Bp65 in the treatment group and the control group before treatment (P0.05). The content of NF- kappa Bp65 in the treatment group was significantly lower than before the treatment (P0.01). There was no difference between the control group and the control group after the treatment. Statistical significance (P0.05), the content of NF- kappa Bp65 in the treatment group was significantly lower than that of the control group (P0.01).7. serum Col- III compared with the control group (P0.01).7. serum Col- III: the level of serum Col- III in the control group was higher than the normal group before treatment, and the difference was significant (P0.01). There was no statistical difference between the treatment group and the control group before the treatment of the serum Col- III level (P0.0). 5) the level of serum Col- III in the treatment group was significantly lower than that before treatment (P0.01). The level of serum Col- III in the control group was not statistically significant (P0.05) after treatment (P0.05). The level of serum Col- III in the treatment group was significantly lower than that in the control group (P0.01).
Conclusion: 1. Qing Shen kidney granules can improve the clinical symptoms of CRF damp heat syndrome, reduce Scr, increase the serum IL-17, NF- kappa Bp65 level of Col- III and PBMC in the patients with eGFR.2.CRF damp heat syndrome, and the.3. clear kidney granules can reduce the serum IL-17, Col- III and the level of nuclear kappa. After treatment, there was no abnormal change in safety index and no adverse drug reaction occurred during the treatment.
Objective: to detect the ratio of auxiliary T lymphocyte (CD4+T cell) to suppressive T lymphocyte (CD8+T cells) in peripheral blood of rats with unilateral ureteral obstruction (UUO) and the ratio of CD4+T cells to T lymphocyte (CD8+T cells) in the peripheral blood of renal interstitial fibrosis (RIF) rats. 17) the expression of nuclear factor kappa B-p65 (nuclear factors- kappa Bp65, NF- kappa Bp65), type III collagen (collagen type III, Col- III), to observe the disorder of immune function in UUO rats, the high release of inflammatory mediators and the intervention of clear kidney granules.
Methods: 60 healthy male SD rats were randomly divided into 5 groups according to the random area group method: the Qing Shen Granule group, the bailing capsule group, the model group, the sham operation group, the normal group, and 12 rats in each group. The blood urea nitrogen nitrogen (BUN) and the serum creatinine (serum creatinine, Scr) were detected before the experiment in each group. The difference was not statistically significant (P0.05). The results were not statistically significant (serum creatinine, Scr). The results were not statistically significant (P0.05). Group, bailing capsule group and model group were prepared with UUO method of renal interstitial fibrosis rat model. The three groups in the sham operation group separated the ureter but did not ligate the ureter before the sham operation group. The Qing Shen Granule group dissolved in the warm water of the 0.6g/100g in the warm water of 4ml, and the bailing capsule group dissolved in the warm water of 4ml in the warm water of 4ml, and the model group was treated with the bailing capsule group. The rats in the sham operation group and the normal group were intragastric with 4ml warm water. The rats in each group were intragastric every day for 3 weeks. The experimental rats were given the blood of the abdominal aorta and the left kidney after the end of the gavage of the stomach. The urine volume of the rats in each group was 24 hours before the operation. The biochemical method was used to detect the BUN, Scr, and 24 hour urine protein. The flow cytometry was used to detect CD4+/CD8+, Th17 cells of peripheral blood. The blood flow cytometry was used to detect CD4+/CD8+, Th17 cells of peripheral blood. The blood flow cytometry was used to detect CD4+/CD8+, Th17 cells of peripheral blood. Renal pathological examination was performed by proportional.HE staining and Masson staining. The expression of IL-17, Col- III and NF- kappa Bp65 in renal tissue were detected by immunohistochemistry.
Results: 1.BUN, Scr comparison: (1) there was no significant difference (P0.05) between the groups of rats before treatment (P0.05). After treatment, the BUN in the Qing Shen Granule group, bailing capsule group and the model group increased significantly (P0.01) compared with the normal group and the sham operation group (P0.01). There was no statistical difference between the rats of the artificial hand group and the normal group (P0.), the difference was not statistically significant (P0.). 05) the BUN in the Qing Shen Granule group and the bailing capsule group were lower than the model group, and the difference was significant (P0.01). The BUN of the Qing Shen Granule group was lower than the bailing capsule group, and the difference was statistically significant (P0.05). (2) there was no significant difference (P0.05) between the Scr in each group of rats before treatment (P0.05). After treatment, the Qing Shen Granule group, bailing capsule group, and the model group Scr were all Scr The difference was significant compared with the normal group and the sham operation group (P0.01). The Scr of the sham operation group was compared with the normal group (P0.05). The Scr of the Qing Shen Granule group and the bailing capsule group was lower than the model group (P0.01). The Scr of the Qing Shen granule group was lower than the bailing capsule group, and the difference was statistically significant (P0.05).2.24. The quantitative comparison of hourly urine protein: the 24 hour urine protein of the model group was higher than that of the normal group and the sham group (P0.01). The 24 hour urine protein of the rats in the Qing Shen Granule group was higher than the normal group (P0.01), and the urine protein of the rats in the group of Qing Shen Granule group was higher than that of the sham group, and the difference was higher than that of the sham operation group. There was statistical significance (P0.05). There was no significant difference between the 24 hours urine protein in the sham operation group and the normal group (P0.05). The urine protein in the Shishen group group and the bailing capsule group were lower than the model group (P0.01). The urine protein quantity of the rats in the Qing Shen Granule group was lower than that in the bailing group. The difference was that the urine protein was lower than that in the bailing group. Statistical significance (P0.05).3. peripheral blood CD4+/CD8+ comparison: the peripheral blood CD4+/CD8+ of the rats in the Qing Shen Granule group was higher than that of the normal group and the sham operation group, the difference was statistically significant (P0.05). The peripheral blood CD4+/CD8+ of the model group was higher than that of the normal group and the sham operation group, and the difference was significant (P0.01). The peripheral blood of the sham operation group was CD4+/CD8+. Compared with the normal group, the difference was not statistically significant (P0.05). The peripheral blood CD4+/CD8+ of the Qing Shen Granule group was lower than the model group (P0.01). The peripheral blood CD4+/CD8+ of the bailing capsule group was lower than the model group (P0.05). The peripheral blood CD4+/CD8+ of the Qing Shen Granule group was lower than that of the bailing capsule group, and the difference was significant (P0.01).4. outside the capsule group. The proportion of Th17 cells in peripheral blood was compared: the proportion of Th17 cells in the group of Qing Shen Granule group and bailing capsule group was higher than that of the normal group and the sham operation group, the difference was significant (P0.01). The proportion of peripheral Th17 cells in the sham operation group was not statistically significant compared with the normal group (P0.05). The proportion of the peripheral Th17 cells in the rats of the Qing Shen Granule group was more than that of the normal group (P0.05). The proportion of the model group was lower than that of the model group (P0.01). The percentage of Th17 cells in the capsule group was lower than that in the model group, and the difference was statistically significant (P0.05). The proportion of the peripheral Th17 cells in the rats of the Qing Shen Granule group was lower than that of the bailing capsule group. The difference was statistically significant (P0.05).5. immunohistochemical IL-17 staining comparison: the Qing Shen Granule group, the bailing capsule group, the model The semi quantitative score of IL-17 in the group of rats was higher than that in the normal group and the sham operation group (P0.01). There was no significant difference in the semi quantitative score of IL-17 in the sham operation group and the normal group (P0.05). The IL-17 semi quantitative score of the Qing Shen Granule group and the bailing capsule group was lower than that of the model group (P0.01). The semi quantitative score of IL-17 in rats was lower than that of Bailing capsule group, and the difference was statistically significant (P0.05).6. immunohistochemical NF- kappa Bp65 staining: the semi quantitative score of NF- kappa Bp65 coloring in the model group was higher than that of the normal group and the sham operation group, the difference was significant (P0.01). The coloring semi quantitative of NF- kappa Bp65 in the sham operation group was half quantitative. Compared with the normal group, the difference was not statistically significant (P0.05). The semi quantitative score of NF- kappa Bp65 coloring in the Qing Shen Granule group and the bailing capsule group was lower than the model group, and the difference was significant (P0.01). The NF- kappa Bp65 coloring semi quantitative score of the rats in the Qing Shen Granule group was lower than that in the bailing group, and the difference was statistically significant (P0.05).7. immunization Col- III Comparison: the Col- III coloring semi quantitative score of the model group was higher than that of the normal group and the sham group (P0.01). The Col- III coloring semi quantitative score of the rats in the Qing Shen Granule group was higher than that of the normal group and the sham operation group (P0.05). The semi quantitative score of Col- III coloring in the sham operation group was and was positive. The difference was not statistically significant (P0.05). The semi quantitative score of Col- III coloring in the group of Qing Shen Granule group and bailing capsule group was lower than that of the model group (P0.01), but the semi quantitative score of Col- III coloring in the rats of the Qing Shen Granule group was lower than that of the bailing group (P0.05).
Conclusion: 1. immuno inflammatory mediating mechanism participates in the pathogenesis of renal interstitial fibrosis,.2. clearing kidney granules can reduce the blood creatinine, urea nitrogen and the reduction of 24h urine protein quantitative.3. clearing kidney granules can reduce the renal pathological damage in renal fibrosis rats, and reduce the inflammatory response by regulating the immune dysfunction and reducing the inflammatory mediations. Release of mass IL-17, inhibition of NF- kappa B
【学位授予单位】：安徽中医药大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R692

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