脂多糖通过上调热休克蛋白27的表达减轻小鼠肾缺血再灌注损伤

发布时间：2018-05-22 18:53

本文选题：肾疾病 + 再灌注损伤　；参考：《第二军医大学学报》2017年01期

【摘要】：目的探讨热休克蛋白27(heat shock protein-27,HSP27)在脂多糖(lipopolysaccharide,LPS)减轻小鼠肾缺血再灌注(ischemia-reperfusion,IR)损伤中的作用。方法雄性C57BL/6小鼠随机分为4组:假手术组、LPS+假手术组、肾IR组和LPS+肾IR组,每组又分为槲皮素(quercetin,200 mg/kg)亚组和溶剂对照亚组。采用右肾切除+左肾肾蒂夹闭25min后再灌注建立肾IR模型,在肾IR前3d腹腔注射LPS(3mg/kg)进行预处理,采用槲皮素(200mg/kg)灌胃抑制HSP27的表达。再灌注后24h,各组小鼠经腹主动脉采血检测血清肌酐(Cr)、尿素氮(BUN)水平评估肾IR损伤模型,取左肾评估炎症反应程度、HSP27蛋白表达水平及凋亡相关蛋白caspase-3的活性。结果 LPS预处理可明显降低肾IR后的血清Cr、BUN水平,并减少肾小管损伤程度,同时能提高肾内HSP27的表达水平(P0.05);槲皮素能明显抑制肾内HSP27的表达水平,且能明显削弱LPS预处理对肾脏IR的减轻作用,包括升高Cr、BUN水平和造成更严重的炎症反应(P0.05)。另外,LPS能明显降低肾脏IR后肾内caspase-3的活性,但槲皮素能明显削弱这种作用(P0.05)。结论 LPS通过上调HSP27的表达减轻小鼠肾脏IR损伤。
[Abstract]:Objective to investigate the role of heat shock protein 27(heat shock protein-27 (HSP27) in reducing renal ischemia-reperfusion injury in mice with lipopolysaccharide (LPS). Methods male C57BL/6 mice were randomly divided into 4 groups: sham operation group, renal IR group and LPS renal IR group. Each group was subdivided into quercetin and quercetin (200 mg / kg) subgroup and solvent control group. Renal IR model was established by right nephrectomy and left renal pedicle occlusion (25min) and reperfusion. The model was pretreated by intraperitoneal injection of LPS3 mg / kg 3 days before IR. Quercetin 200 mg / kg was administered to stomach to inhibit the expression of HSP27. 24 hours after reperfusion, the serum creatinine (Cr) and urea nitrogen (bun) were collected from the abdominal aorta of each group to evaluate the renal IR injury model. The level of HSP27 protein expression and the activity of apoptosis-related protein (caspase-3) were measured in the left kidney. Results LPS pretreatment could significantly reduce the level of Cr bun, decrease the degree of renal tubule injury and increase the expression of HSP27 in the kidney (P 0.05), quercetin could significantly inhibit the expression of HSP27 in the kidney. And it can obviously weaken the effect of LPS pretreatment on renal IR, including raising the level of Cr bun and causing more serious inflammatory reaction (P 0.05). In addition, lipopolysaccharide could significantly reduce the activity of caspase-3 in the kidney after IR, but quercetin could significantly weaken this effect. Conclusion LPS can attenuate renal IR injury by up-regulating the expression of HSP27.
【作者单位】：上海交通大学医学院附属仁济医院肝脏外科;
【基金】：上海市卫生和计划生育委员会青年科研项目(20124Y130)~~
【分类号】：R692

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