尿毒症毒素对甲酚对单核细胞体外释放炎症因子的影响

发布时间：2018-05-22 16:10

本文选题：单核细胞 + 尿毒症　；参考：《解放军医学杂志》2015年01期

【摘要】：目的利用体外细胞模型研究尿毒症毒素对甲酚对单核细胞释放炎症因子的影响及其可能机制。方法采用CCK-8法检测THP-1单核细胞株经20、40、80、160mg/L对甲酚分别处理6、12、24h后细胞增殖能力的变化;根据细胞增殖实验结果,选取40mg/L和80mg/L两个剂量组作为低剂量组和高剂量组,提取细胞RNA和蛋白质,用RT-PCR法、Western blotting检测对甲酚处理24h后THP-1单核细胞株炎症因子TNF-α、抗炎因子IL-10以及炎症相关Toll样受体4(TLR-4)m RNA和蛋白表达的变化。结果对甲酚可抑制THP-1细胞增殖,其抑制作用呈浓度和时间依赖性(P0.05);RT-PCR及Western blotting检测显示,对甲酚处理的THP-1细胞炎症因子TNF-α释放增加,IL-10释放减少,TLR-4表达增加(P0.05)。结论对甲酚可抑制THP-1细胞增殖,抑制IL-10抑炎因子表达,并可能通过上调TLR-4的表达,促进促炎因子TNF-α的释放,从而导致微炎症状态。
[Abstract]:Objective to study the effect of uraemia toxin on the release of inflammatory cytokines from monocytes by cresol in vitro. Methods CCK-8 assay was used to detect the changes of cell proliferation ability of THP-1 monocytes treated with 20 ~ 40 ~ 80160 mg / L p-cresol for 24 h, respectively. According to the results of cell proliferation test, two dose groups of 40mg/L and 80mg/L were selected as low dose group and high dose group. RNA and protein were extracted, and the expression of inflammatory factor TNF- 伪, anti-inflammatory factor IL-10, Toll like receptor 4(TLR-4)m RNA and protein in THP-1 monocytes treated with p-cresol for 24 h were detected by RT-PCR method. Results p-cresol inhibited the proliferation of THP-1 cells in a dose-and time-dependent manner. The results of RT-PCR and Western blotting showed that the release of TNF- 伪 in THP-1 cells treated with p-cresol increased the release of TNF- 伪 and the decrease of TLR-4 expression. Conclusion p-cresol can inhibit the proliferation of THP-1 cells and inhibit the expression of IL-10 anti-inflammatory factor, which may promote the release of proinflammatory factor TNF- 伪 by up-regulating the expression of TLR-4.
【作者单位】：第三军医大学附属新桥医院肾内科、全军肾脏病中心、重庆市肾脏病研究所、国家中医药管理局重点专科;第三军医大学预防医学系毒理学研究所;
【分类号】：R692.5

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