miRNA-15a在前列腺癌中的表达及诊断意义

发布时间：2018-05-24 21:04

  本文选题：前列腺癌 + 良性前列腺增生　； 参考：《中国免疫学杂志》2016年08期

【摘要】：目的:检测前列腺癌患者miRNA-15a的表达情况,探讨其在前列腺癌诊断的意义。方法:选择2014年1月至2015年1月本院泌尿外科收治的前列腺癌患者血清及肿瘤组织36例,良性前列腺增生患者(Prostatic hyperplasia,BPH)血清40例,健康对照血清40例。miRNA-15a表达采用实时定量聚合酶链反应(Real-time PCR)技术检测。结果:miRNA-15a在前列腺癌患者、良性前列腺增生患者和健康对照血清的表达量分别为(0.193±0.081)、(0.359±0.04)和(0.376±0.037),miRNA-15a在前列腺癌患者血清中的表达量显著低于良性前列腺增生患者和健康对照(P0.05),在BPH组和对照组中的表达差异无统计学意义(P0.05),前列腺癌患者血清miRNA-15a表达在不同PSA表达水平、Gleason评分、临床分期、有无远处转移之间差异有统计学意义(P0.05),与其他病理因素无关(P0.05);前列腺癌患者肿瘤组织中的miRNA-15a的表达与PSA表达水平、Gleason评分、临床分期、有无远处转移之间差异有统计学意义(P0.05),与其他病理因素无关(P0.05)。结论:miRNA-15a低表达参与了前列腺癌的发生、发展过程,是前列腺癌辅助诊断及治疗的潜在的指标。
[Abstract]:Objective: to detect the expression of miRNA-15a in patients with prostate cancer and to explore its significance in the diagnosis of prostate cancer. Methods: 36 patients with prostate cancer, 40 cases of benign prostatic hyperplasia (Prostatic hyperplasia, BPH), 40 cases of healthy control serum,.MiR were selected from January 2014 to January 2015. The expression of NA-15a was detected by real-time quantitative polymerase chain reaction (Real-time PCR). Results: the expression of miRNA-15a in patients with prostate cancer, benign prostatic hyperplasia and healthy control was (0.193 + 0.081), (0.359 + 0.04) and (0.376 + 0.037). The expression of miRNA-15a in the serum of prostate cancer patients was significantly lower than that of benign prostatic cancer. There was no significant difference in the expression of prostatic hyperplasia and healthy control (P0.05) in the BPH group and the control group (P0.05). The expression of miRNA-15a in the serum of the prostate cancer patients at different levels of PSA expression, the Gleason score, the clinical stage, and the distant metastasis were statistically significant (P0.05), and were not related to other pathological factors (P0.05). The expression of miRNA-15a in the tumor tissues of the patients with adenocarcinoma was correlated with the level of PSA expression, Gleason score, clinical stage, and distant metastasis (P0.05), not related to other pathological factors (P0.05). Conclusion: the low expression of miRNA-15a is involved in the development of prostate cancer, the development process, and the potential of auxiliary diagnosis and treatment of prostate cancer. Indicators.
【作者单位】： 武汉科技大学医学院;湖北省黄石市爱康医院;
【分类号】：R737.25
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本文编号：1930536