远程缺血预处理对小鼠肾缺血再灌注损伤的保护作用和可能机制的研究

发布时间：2018-06-09 23:55

本文选题：远程缺血预处理 + 缺血再灌注损伤　；参考：《浙江大学》2014年硕士论文

【摘要】：目的：通过建立肾脏缺血再灌注损伤动物模型,研究肢体远程缺血预处理对小鼠肾缺血再灌注损伤的保护作用,并探讨其可能的机制。实验方法：C57BL/6雄性小鼠15只,8～10周龄,体重20～25g,随机分为假手术组(Sham组)、缺血再灌注组(I/R组)、肢体远程缺血预处理组(RIPC组),每组5只。假手术组(Sham组)打开腹腔,分离左侧肾蒂,但不阻断肾蒂,旷置观察35min；缺血再灌注组(I/R)打开腹腔,分离左侧肾蒂,用无损动脉夹夹闭左侧肾蒂,35min后去除动脉夹并切除右侧肾脏,再灌注24h；肢体远程缺血预处理组(RIPC组)：用橡皮经结扎小鼠下肢根部5min,松开5min,反复4次行肢体远程缺血预处理,在最后一次5min再灌注后立即打开腹腔,分离左侧肾蒂,用无损动脉夹阻断左侧肾蒂,35min后去除动脉夹并切除右侧肾脏,再灌注24h。于再灌注24h后眼眶取血留取血清标本测定血清肌酐及尿素氮,采用Luminex的方法检测血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、白细胞介素-10(IL-10)浓度。处死小鼠留取肾组织伊红染色观察肾组织病理学。结果：再灌注24h后,缺血再灌注组与假手术组相比,血清肌酐浓度、尿素氮浓度和肾组织病理学评分均显著升高(p0.05),血清TNF-α、IL-6、浓度均升高(p0.05),血清IL-10浓度差异无统计学意义,(p0.05)；与缺血再灌注组比较,远程缺血预处理组血清肌酐、尿素氮浓度及肾组织病理学评分均降低,血清TNF-α、IL-6浓度降低,血清IL-10浓度升高(p0.05)。结论：肢体远程缺血预处理对小鼠肾脏缺血再灌注损伤具有保护作用。肢体远程缺血预处理可能通过抑制促炎症细胞因子TNF-α、IL-6的释放,增加抗炎症因子IL-10的合成,调整机体促炎症与抗炎症的平衡来实现肾脏的保护作用。
[Abstract]:Objective: to study the protective effect of limb remote ischemic preconditioning on renal ischemia-reperfusion injury in mice by establishing an animal model of renal ischemia-reperfusion injury, and to explore its possible mechanism. The rats were randomly divided into sham-operated group, ischemia-reperfusion group and limb remote ischemic preconditioning group, with 5 rats in each group. Sham group (sham group) opened the abdominal cavity, separated the left renal pedicle, but did not block the renal pedicle, and observed the left renal pedicle for 35 min. In the ischemia reperfusion group, I / R) opened the abdominal cavity, separated the left renal pedicle, clipped the left renal pedicle for 35 minutes and removed the artery clip and removed the right kidney. Limb remote ischemic preconditioning group (RIPC group): ligated the lower extremity root of mice with rubber for 5 minutes, loosened for 5 minutes, repeated four times of limb remote ischemic preconditioning, immediately after the last 5min reperfusion, opened the abdominal cavity and separated the left renal pedicle. The left renal pedicle was blocked with a non-invasive artery clip for 35 minutes, then the artery clip was removed and the right kidney was resected for 24 h after reperfusion. Serum creatinine and urea nitrogen were measured 24 hours after reperfusion. Serum levels of TNF- 伪, IL-6 and IL-10 were determined by Luminex method. Results: 24 hours after reperfusion, serum creatinine concentration in ischemia reperfusion group was higher than that in sham operation group. The concentrations of urea nitrogen and renal histopathology were significantly increased, serum TNF- 伪 and IL-6 were increased, and the concentrations of IL-10 were not significantly different from those in the ischemic reperfusion group, compared with those in the ischemic reperfusion group, the serum creatinine in the remote preconditioning group was higher than that in the ischemic preconditioning group, and there was no significant difference in the concentration of IL-10 between the two groups. The concentrations of urea nitrogen and renal histopathology were decreased, the concentration of serum TNF- 伪 and IL-6 decreased, and the concentration of serum IL-10 increased (p0.05). Conclusion: limb remote ischemic preconditioning has protective effect on renal ischemia-reperfusion injury in mice. Limb remote ischemic preconditioning may protect the kidney by inhibiting the release of TNF- 伪 IL-6, increasing the synthesis of anti-inflammatory cytokine IL-10 and adjusting the balance between pro-inflammatory and anti-inflammatory.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R692

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