megsin干扰质粒对大鼠Thy1肾炎病变的拮抗作用

发布时间：2018-06-15 01:11

本文选题：megsin干扰质粒 + Thy肾炎　；参考：《复旦学报(医学版)》2017年01期

【摘要】：目的应用大鼠Thy1肾炎模型体外输入megsin siRNA干扰质粒,观察下调megsin表达后对大鼠肾炎中肾小球系膜细胞和基质增生的抑制作用。方法雄性SD大鼠共9只,随机等分为对照组(A组),Thy1肾炎模型组(B组)和质粒组(C组)。C组为肾炎模型加肾动脉注射megsin siRNA质粒,并辅以电穿孔技术促进质粒浸入肾脏。肾脏标本行real-time PCR、HE染色、PAS染色和megsin、PCNA的免疫组化染色。结果与对照组比较,HE染色结果可见B组肾小球系膜细胞明显增生伴基质增多,而C组肾小球细胞数量明显少于B组,但多于A组;PAS染色可见B组肾小球系膜区基质大量增多,呈节段性分布,C组则与之相比基质增生减少;RT-PCR和免疫组化结果显示B组肾小球中megsin mRNA和蛋白质水平明显升高,与A组相比两指标差异均有统计学意义(P0.01),而在C组megsin mRNA和蛋白质水平又明显下降,与A、B组相比两指标差异有统计学意义(P0.05)。PCNA阳性细胞核平均数A组为3个,B组14.79个,而C组仅为6.94个。统计显示B组和A组、B组和C组之间差异均有统计学意义(P0.001)。结论注射megsin干扰质粒以后,系膜细胞、基质的增生均受到抑制,肾炎病变减轻,提示megsin干扰质粒体外转染具有拮抗大鼠Thy1肾炎的作用,这为探索以系膜细胞增生为特征的肾脏疾病的防治提供了新的思路和依据。
[Abstract]:Objective to investigate the inhibitory effect of megsin siRNA interference plasmid on Mesangial cells and matrix proliferation in rat glomerulonephritis after down-regulation of megsin expression in vitro. Methods Nine male Sprague-Dawley rats were randomly divided into two groups: control group (group A) and control group (group B) and plasmid group C (group C): nephritis model and renal artery injection of megsin siRNA plasmid, supplemented with electroporation technique to promote plasmid immersion into the kidney. The renal specimens were stained by pas and immunohistochemical staining of megsinia megsini. Results compared with the control group, the glomerular Mesangial cells in group B were obviously proliferated and matrix increased, while the number of glomerular cells in group C was significantly less than that in group B, but more in group A than in group A. The results of RT-PCR and immunohistochemistry showed that the levels of megsin mRNA and protein in glomeruli in group B were significantly higher than those in group C. Compared with group A, there were significant differences in megsin mRNA and protein levels between group A and group A (P 0.01), and there was a significant difference between group A and group A (P 0.05). The average number of megsin positive nuclei in group A was 14.79 in group A, and in group A, there were significant differences between group A and group A (P < 0.05). In group C, only 6.94. Statistical analysis showed that there were significant differences between group B and group A, group B and group C (P 0.001). Conclusion the proliferation of Mesangial cells and matrix was inhibited after injection of megsin interference plasmid, and the pathological changes of glomerulonephritis were alleviated. It suggested that megsin interference plasmid transfection in vitro could antagonize Thy1 glomerulonephritis in rats. This provides a new idea and basis for the prevention and treatment of renal diseases characterized by Mesangial cell proliferation.
【作者单位】：复旦大学上海医学院临床医学系;复旦大学基础医学院病理学系;
【基金】：国家基础科学人才培养基金能力提高项目(J1210041)~~
【分类号】：R692.3

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