男性不育患者精子DNA损伤与非整倍体精子的研究

发布时间：2018-06-15 04:34

  本文选题：不育症 + DNA损伤　； 参考：《安徽医科大学》2014年硕士论文

【摘要】：目的：运用精子染色质扩散实验(SCD)分析精子DNA损伤率和荧光原位杂交实验(FISH)分析精子染色体（18、X、Y）非整倍体率。探讨并分析男性不育患者精子DNA完整性和染色体畸变的发生率及临床意义。 方法：精液标本来自正常已育的男性及不育男性患者，依据WHO标准行精液分析，根据精液分析结果及不育史分为对照组、少精症组、弱精症组、少弱精症组。精子染色质扩散实验（SCD）分析精子DNA损伤率和荧光原位杂交实验（FISH）分析精子染色体（18、X、Y）非整倍体率。 结果：经统计学分析表明少精症组、弱精症组和少弱精症组患者精子DNA损伤率对照组相比差异有显著统计学意义（P＜0.01）；不育组中的精子染色体（18、X、Y）非整倍体率与对照组比较差有统计学意义（P＜0.05）；精子DNA损伤率与染色体非整倍体率之间呈显著正相关（r=0.453，P=0.008，n=33）。 结论：不育患者精子DNA损伤率和染色体非整倍体率较正常对照组明显升高且具有显著的正相关性。在生殖细胞减数分裂过程中精子DNA损伤可能是导致非整倍体精子形成的重要原因之一。
[Abstract]:Objective: to analyze the rate of DNA damage in sperm by sperm chromatin diffusion assay (SCD) and the rate of aneuploidy by fluorescence in situ hybridization (fish). To investigate and analyze the incidence and clinical significance of sperm DNA integrity and chromosome aberration in infertile men. Methods: semen samples were collected from normal fertile men and infertile men. Semen analysis was performed according to WHO standard. According to the results of semen analysis and history of infertility, semen samples were divided into control group, oligozoospermia group and oligozoospermia group. Sperm chromatin diffusion assay (SCD) and fluorescence in situ hybridization (fish) were used to analyze the rate of DNA damage and aneuploidy. Results: the difference of sperm DNA damage rate between oligozoospermia group, asthenospermia group and oligozoospermia group was significant (P < 0.01). The rate of aneuploidy in infertile group was significantly lower than that in control group (P < 0.05), and there was a significant positive correlation between DNA damage rate and chromosome aneuploidy rate (P < 0.05), and there was a significant positive correlation between sperm DNA damage rate and chromosome aneuploidy rate (P < 0.05), and there was a significant positive correlation between sperm DNA damage rate and chromosome aneuploidy rate (P < 0.05). Conclusion: sperm DNA damage rate and chromosome aneuploid rate in infertile patients were significantly higher than those in normal controls. DNA damage of sperm during meiosis may be one of the important reasons for aneuploidy spermatogenesis.
【学位授予单位】：安徽医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R698.2

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