慢性前列腺炎患者精子DNA的损伤及临床意义的初步研究

发布时间：2018-06-15 16:09

本文选题：慢性前列腺炎 + 精子　；参考：《南华大学》2014年硕士论文

【摘要】：目的：探讨不同原因的慢性前列腺炎患者是否合并精子DNA的损伤，进一步了解不同原因的慢性前列腺炎所引起的精子DNA损伤有无不同。方法：通过对160例纳入患者按前列腺液常规的化验结果进行慢性前列腺炎筛选和精液常规分析，按其前列腺液化验结果将其分为慢性前列腺炎合并细菌感染组（A组40例）、慢性前列腺炎合并支原体感染组（B组40例）、慢性前列腺炎合并其他原因组（C组40例）以及健康对照组（D组40例）。然后采用精子染色质扩散试验和彗星实验2种方法分别检测患者精子DNA的完整性，其中精子染色质扩散实验以精子DNA断裂指数表示其损伤程度，，即精子DNA损伤定性实验；彗星实验以彗星尾部DNA含量的百分比表示其损伤程度，对精子染色质扩散实验的结果进行进一步验证，即精子DNA损伤定量实验。最后通过统计分析得出A、B、C、D四组是否合并精子DNA损伤及组组之间精子DNA损伤的程度，进一步了解不同原因慢性前列腺炎所引起的精子DNA损伤有无不同。结果：对160例病例行精子染色质扩散实验得出：细菌感染组、支原体感染组、其他原因组SCD损伤率高于健康对照组SCD损伤率，差异有统计学意义（p0.005）。细菌感染组SCD损伤率高于支原体感染组、其他原因组SCD损伤率，差异有统计学意义（p0.005）。支原体感染组SCD损伤率与其他原因组SCD损伤率比较无统计学意义（p0.05）。彗星实验得出：细菌感染组彗星尾长百分比为47.44±25.80，支原体感染组彗星尾长百分比为22.96±21.40，其他原因组彗星尾长百分比为22.02±21.92，健康对照组彗星尾长百分比为6.51±9.85。细菌感染组、支原体感染组、其他原因组彗星尾长百分比高于健康对照组彗星尾长百分比，差异有统计学意义（p0.005）。细菌感染组彗星尾长百分比高于支原体感染组和其他原因组彗星尾长百分比，差异有统计学意义（p0.005），支原体感染组彗星尾长百分比与其他原因组彗星尾长百分比比较无统计学意义（p0.05）。可认为：各实验组与对照组之间精子DNA损伤有差别，各实验组精子DNA损伤率高于健康对照组。A组的精子DNA损伤程度高于B组、C组的精子DNA损伤。但还不能认为B组与C组的精子DNA损伤率有差别。结论： 1.慢性前列腺炎可以引起不同程度的精子DNA损伤。 2.引起慢性前列腺炎的原因不同，精子DNA损伤程度有所不同，其中以细菌感染所致精子DNA损伤程度最严重，支原体感染和其他原因所致的慢性前列腺炎精子DNA也有不同程度的损伤。 3.精子DNA损伤的检测可能成为临床上研究男性不育的一个重要的指标。
[Abstract]:Objective: to investigate whether the patients with chronic prostatitis have different sperm DNA damage, and to find out if there are differences in sperm DNA damage caused by chronic prostatitis. Methods: chronic prostatitis screening and semen routine analysis were carried out in 160 patients with prostatic fluid. According to the test results of prostatic fluid, it was divided into four groups: chronic prostatitis with bacterial infection group (n = 40), chronic prostatitis with mycoplasma infection group (n = 40), chronic prostatitis with other causes group (n = 40) and chronic prostatitis with mycoplasma infection group (n = 40). And healthy control group (n = 40). Then sperm chromatin diffusion test and comet assay were used to detect the integrity of sperm DNA respectively. The sperm chromatin diffusion test showed the degree of damage by sperm DNA break index, that is, the qualitative test of sperm DNA damage. The percentage of DNA content in comet tail indicated the degree of damage. The result of sperm chromatin diffusion test was further verified, that is, the quantitative assay of sperm DNA damage. Finally, the statistical analysis was conducted to find out whether the four groups were combined with sperm DNA damage and the degree of sperm DNA damage between groups, and to find out whether there were differences in sperm DNA damage caused by chronic prostatitis. Results: the sperm chromatin diffusion test in 160 cases showed that the SCD damage rate in bacterial infection group, mycoplasma infection group and other cause group was higher than that in healthy control group, and the difference was statistically significant (P 0.005). The SCD damage rate of bacterial infection group was higher than that of mycoplasma infection group, and the difference of SCD damage rate in other cause group was statistically significant (P 0.005). The SCD damage rate of mycoplasma infection group was not significantly higher than that of other causes group (P 0.05). Comet assay showed that the percentage of comet tail length was 47.44 卤25.80 in bacterial infection group, 22.96 卤21.40 in mycoplasma infection group, 22.02 卤21.92 in other cause groups and 6.51 卤9.85 in healthy control group. The percentage of comet tail length in bacterial infection group, mycoplasma infection group and other cause group was higher than that in healthy control group (P 0.005). The percentage of comet tail length in bacterial infection group was higher than that in mycoplasma infection group and other cause group. The percentage of comet tail length in mycoplasma infected group was not significantly higher than that in other cause groups (p 0.05). It can be concluded that the sperm DNA damage rate of each experimental group is higher than that of healthy control group. The degree of sperm DNA damage in group A is higher than that in group B and C. But it can not be concluded that there is difference between group B and group C in sperm DNA damage rate. Conclusion: 1. Chronic prostatitis can cause different degrees of sperm DNA damage. 2. The causes of chronic prostatitis are different, and the degree of sperm DNA damage is different, among which the degree of sperm DNA damage caused by bacterial infection is the most serious. Mycoplasma infection and other causes of chronic prostatitis caused by sperm DNA damage to varying degrees. The detection of sperm DNA damage may be an important marker for the clinical study of male infertility.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R697.33

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