大鼠睾丸开放活检导致生精小管形态改变

发布时间：2018-06-17 20:12

本文选题：大鼠 + 睾丸　；参考：《川北医学院》2017年硕士论文

【摘要】：研究背景及目的:笔者以前参与的研究表明,成年大鼠睾丸开放活检,导致了生精细胞团向生精小管腔内突出的形态改变。本文拟进一步研究确定大鼠睾丸活检导致的生精小管形态改变,同时利用连续切片观察生精小管的形态以探讨活检所致形态改变的形成机制。材料与方法:14只成年大鼠麻醉后在一侧睾丸作切口挤出组织块,浸润固定整个睾丸后切取挤出的组织块(未游离活检块)。另8只成年大鼠麻醉后在一侧睾丸作切口挤出组织块并立即切下,然后固定(游离活检块)。这22只动物的另一侧睾丸,浸润固定后切取睾丸组织块(原位组织块)。所有组织块都用甲基丙烯酸树脂包埋,从每个包埋块切取1张切片(25μm厚)。另外,从每个游离活检块再切平均46张连续切片。所有切片用过碘酸-席夫试剂和苏木精染色,然后进行组织学观察分析。结果:与原位组织块相比,未游离活检块的生精小管基本正常,而在切自游离活检块的切片上,有约一半的生精小管轮廓腔内可见单独的生精细胞团,这些生精细胞团几乎都是由精母细胞及精子细胞组成。连续切片观察提示,这些生精细胞团来自某处的生精小管壁,开放活检过程中被挤出或突向邻近的生精小管腔。结论:成熟睾丸开放活检可导致生精细胞团从生精上皮脱落,突向管腔,这可能是活检时游离组织块内生精小管的局部收缩所致。
[Abstract]:Background and objective: open biopsy of testis in adult rats has resulted in morphological changes of spermatogenic cell clusters protruding into seminiferous tubules. This paper intends to further study and determine the morphological changes of seminiferous tubules induced by testicular biopsy in rats, and observe the morphology of seminiferous tubules by serial sections in order to explore the formation mechanism of the morphological changes caused by testicular biopsies. Materials and methods 14 adult rats were anesthetized and extruded tissue mass (not free biopsy mass) in one testicle was cut out by infiltrating and fixing the whole testis. The other 8 adult rats were anesthetized and cut out the tissue mass through a unilateral testicular incision and then fixed (free biopsy mass). On the other side of the testis of the 22 animals, the testicular mass (in situ tissue mass) was removed after invasion and fixation. All tissue blocks were encapsulated with methacrylic resin, and a slice of 25 渭 m thick was cut from each embedding block. In addition, an average of 46 consecutive sections were resliced from each free biopsy mass. All sections were stained with iodate-Schiff reagent and hematoxylin. Results: compared with the in situ tissue mass, the seminiferous tubules without free biopsy mass were basically normal, but in the section cut from the free biopsy mass, about half of the seminiferous tubules could be seen in the contours of seminiferous tubules. These spermatogenic cell clusters are almost composed of spermatocytes and sperm cells. Serial microscopic observations suggest that these spermatogenic cell clusters come from somewhere in the seminiferous tubule wall and are extruded or protruded into the adjacent spermatogenic tubules during open biopsy. Conclusion: open biopsy of mature testis can cause spermatogenic cell mass to fall off from spermatogenic epithelium and protrude to lumen, which may be due to the local contraction of endophytic tubules in free tissue mass during biopsy.
【学位授予单位】：川北医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R699.8

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