染料木素对尿毒症大鼠细胞外基质沉积的影响

发布时间：2018-06-20 15:13

  本文选题：染料木素 + 尿毒症　； 参考：《重庆医学》2017年07期

【摘要】：目的 探讨染料木素对尿毒症大鼠细胞外基质(ECM)沉积的影响及其机制。方法 5/6肾切除术建立尿毒症模型鼠,将模型鼠分为染料木素组(G组)和对照组(C组),假手术组(S组)作为正常对照。检测各组术前及术后第4、8周尿蛋白和生化等指标,第8周观察肾组织病理变化情况,半定量法评估肾小球硬化指数(GSI)。免疫组化法检测Ⅳ型胶原(ColⅣ)、纤维连接蛋白(FN)的沉积情况。应用逆转录聚合酶链反应(RT-PCR)法、Western blot法分别检测肾组织中转化生长因子β1(TGF-β1)mRNA转录、蛋白质表达情况。结果 术后第4周与C组比较,G组尿蛋白排泄量减少[(11.63±2.07)vs.(19.93±3.19)mg/d,P0.01],血清尿素氮、肌酐水平降低[(9.39±0.59)vs.(12.09±0.78)mmol/L,(65.11±3.79)vs.(77.63±3.20)μmol/L,P0.01]。至术后第8周,尿蛋白排泄量减少,血清尿素氮、肌酐水平降低更为显著。肾小球内ColⅣ和FN沉积减少[(17.30±1.96)%vs.(24.68±3.97)%,(18.26±2.31)%vs.(29.35±4.15)%,P0.01],肾小球硬化明显减轻。TGF-β1mRNA、蛋白质表达减弱(P0.05)。结论 染料木素可以减少尿毒症大鼠细胞ECM的蓄积,对肾脏具有保护作用。
[Abstract]:Objective to investigate the effect and mechanism of genistein on ECM deposition in uremic rats. Methods the uremic model rats were established by 5 / 6 nephrectomy. The rats were divided into genistein group (group G), control group (group C) and sham operation group (group S) as normal control. Urine protein and biochemical indexes were measured before and after operation, renal pathological changes were observed at the 8th week, and glomerulosclerosis index (GSI) was evaluated by semi-quantitative method. The deposition of Col 鈪，

本文编号：2044690