SphK1、S1PR及α-SMA在IgA肾病中的表达及相关性研究

发布时间：2018-07-06 16:19

  本文选题：IgAN + SphK1　； 参考：《川北医学院》2017年硕士论文

【摘要】：目的:检测鞘氨醇激酶-1(SphK1)、1-磷酸鞘氨醇受体(S1PR)及α-平滑肌肌动蛋白(α-SMA)在IgA肾病(IgAN)患者肾组织中的表达,探讨SphK1信号通路在IgAN发生发展中的作用,为IgAN临床治疗提供实验依据。方法:选取18例肾穿刺活检确诊为IgAN患者肾组织为实验组;5例肾肿瘤患者手术切除远离病变部位的正常组织为对照组。收集两组患者的临床资料,如24小时尿蛋白、血肌酐、尿素氮等。采用免疫组织化学方法检测肾组织中SphK1、S1PR1、S1PR2及α-SMA的表达。采用Image-ProPlus图像分析软件分析光密度值代替相应指标表达量。相关分析法分析SphK1与α-SMA和临床资料的相关性。所有数据采用spass18.0软件进行统计分析,计量资料以均数±标准差((?)±s)表示,实验组和对照组比较采用独立样本t检验,相关性分析使用Pearson相关检验,p0.05提示差异有统计学意义。结果:1.在IgAN患者肾组织中SphK1、S1PR2及α-SMA表达增加,S1PR1/S1PR2的比值降低,与对照组比较,差异具有统计学意义(P0.05);S1PR1表达水平与对照组比较,差异无统计学意义(P0.05)。2.SphK1与α-SMA表达水平、24小时尿蛋白及血肌酐呈正相关(p0.01或p0.05);SphK1与尿素氮无直线相关关系(P0.05)。结论:1.SphK1信号通路可能参与了IgAN发病机制;2.SphK1信号通路在IgAN中的作用可能与α-SMA引起系膜细胞表型改变有关;3.1-磷酸鞘氨醇(S1P)与其受体亚型结合过程中可能存在S1PR1/S1PR2差异性转导;4.肾组织中SphK1表达水平在一定程度上可反映肾脏损害程度;
[Abstract]:Objective: to investigate the expression of sphingosine kinase 1 (SphK1) -1-sphingosine receptor (S1PR) and 伪 -smooth muscle actin (伪 -SMA) in renal tissue of patients with IgA nephropathy (IgAN), and to explore the role of SphK1 signal pathway in the pathogenesis and development of IgAN, and to provide experimental evidence for the clinical treatment of IgAN. Methods: 18 patients with IgAN confirmed by renal biopsy were selected as control group. The clinical data of the two groups were collected, such as 24 hours urine protein, serum creatinine, urea nitrogen, etc. Immunohistochemical method was used to detect the expression of SphK1, S1PR1, S1PR2 and 伪 -SMA in renal tissues. Image-ProPlus image analysis software was used to analyze the optical density instead of the corresponding index expression. The correlation between SphK1 and 伪 -SMA and clinical data was analyzed by correlation analysis. All the data were statistically analyzed by spass18.0 software. The measured data were expressed as mean 卤standard deviation (?) 卤s). The experimental group and the control group were compared with the control group by using independent sample t test. The correlation analysis using Pearson correlation test suggested that the difference was statistically significant. The result is 1: 1. The expression of SphK1P1PR2 and 伪 -SMA in renal tissue of IgAN patients increased and the ratio of S1PR1 / S1PR2 decreased. The difference was statistically significant compared with the control group (P0.05) and the expression level of S1PR1 was higher than that of the control group. There was no significant difference between SphK1 and 伪 -SMA expression (P0.05). There was no linear correlation between SphK1 and urea nitrogen (P0.05). Conclusion: 1. SphK1 signaling pathway may be involved in the pathogenesis of IgAN. Secondly, the role of SphK1 signaling pathway in IgAN may be related to the phenotypic changes of Mesangial cells induced by 伪 -SMA. There may be S1PR1 / S1PR2 differential transduction during the binding of sphingosine (S1P) to its receptor subtype. The expression level of SphK1 in renal tissue can reflect the degree of renal damage to some extent.
【学位授予单位】：川北医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R692.31

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