扩增活化的自体淋巴细胞对裸鼠肾母细胞瘤移植瘤生长的影响

发布时间：2018-07-07 11:49

本文选题：肾母细胞瘤 + 扩增活化的自体淋巴细胞　；参考：《广西医科大学》2017年硕士论文

【摘要】：目的初步探讨扩增活化的自体淋巴细胞(expanded activated autologous lymphocytes,EAAL)对裸鼠肾母细胞瘤(Wilms' tumor,WT)移植瘤生长的影响。方法实验中肿瘤组织来源于术前穿刺活检病理证实肾母细胞瘤的患者,术中切取部分肿瘤组织并制备保存。将2-3块约1mm*1mm*1mm大小的肾母细胞瘤肿瘤组织种植在右腋窝皮下,并将伤口严密缝合,随后将裸鼠放回笼内饲养并严密观察裸鼠的生长变化。小鼠瘤体模型建立后,选取其中15只裸鼠随机分为3组。不同处理组经尾静脉分别输注等量且不同剂量的EAAL细胞悬浮液和等量生理盐水,根据对每组裸鼠不同的处理方式,分为低剂量组、高剂量组和阴性对照组。每组裸鼠经8次注射,末次注射l周后颈椎脱臼法处死裸鼠,剥离肿瘤组织并称重,用游标卡尺准确测量肿瘤直径。采用配对t检验比较每组裸鼠处理前后的差异变化。单因素方差分析比较不同处理组间的差异变化。P0.05确定为差异有统计学意义。结果实验结果表明:注射EAAL细胞和生理盐水后,比较各组间裸鼠肿瘤直径变化有差异,且低、高剂量组较阴性对照组裸鼠肿瘤直径缩小明显(0.97±0.10cm、0.64±0.07 cm vs 1.33±0.09 cm;P0.05)。瘤体质量方面,低、高剂量组亦较阴性对照组裸鼠明显减轻(0.96±0.09g、0.62±0.05g vs 1.20±0.11g;P0.05)。比较注射EAAL细胞两组,高剂量组比低剂量组对肿瘤的抑制作用明显。结论EAAL细胞治疗能够影响裸鼠肾母细胞瘤移植瘤的生长,且可能对裸鼠肾母细胞瘤移植瘤的生长有抑制作用。
[Abstract]:Objective to investigate the effect of amplified activated autologous lymphocyte (expanded activated autologous lymphocytes) on the growth of xenografts in nude mice with Wilms' tumor. Methods tumor tissues were obtained from patients with nephroblastoma confirmed by biopsy before operation. Two or three pieces of tumor tissue about the size of 1mm*1mm*1mm were implanted in the right armpit subcutaneously and the wound was sutured closely. Then the nude mice were reared in cage and the growth of nude mice was observed closely. After the establishment of mouse tumor model, 15 nude mice were randomly divided into 3 groups. The EAAL cell suspensions and the same amount of normal saline were infused into the caudal vein in different groups. According to the different treatment methods of each group, they were divided into three groups: low dose group, high dose group and negative control group. The nude mice in each group were injected for 8 times and the cervical spine dislocations were performed after the last injection for 1 week. The tumor tissues were removed and weighed. The tumor diameter was accurately measured with Vernier caliper. Paired t test was used to compare the difference before and after treatment in each group of nude mice. Univariate ANOVA compared the differences between different treatment groups. P05 was determined to be the difference was statistically significant. Results the results showed that the diameter of tumor in the high dose group was significantly smaller than that in the negative control group (0.97 卤0.10 cm ~ (-1) 卤0.07 cm vs 1.33 卤0.09 cm ~ (-1) P 0.05) after injection of EAAL cells and normal saline. The tumor diameter of the nude mice was lower than that of the control group (0.97 卤0.10 cm ~ (-1) vs 1.33 卤0.09 cm ~ (-1). The tumor mass in the low and high dose group was significantly reduced compared with that in the negative control group (0.96 卤0.09 g / kg vs 1.20 卤0.11 g / g, P 0.05). Compared with EAAL cells injection group, the high dose group was more effective than the low dose group on tumor inhibition. Conclusion EAAL cell therapy can affect the growth of xenografts of nephroblastoma in nude mice and may inhibit the growth of xenografts of nephroblastoma in nude mice.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R737.11

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