小鼠糖尿病肾病足细胞NOD2 mRNA表达变化及其意义

发布时间：2018-07-07 16:26

本文选题：糖尿病肾病 + 足细胞　；参考：《山东医药》2017年03期

【摘要】：目的观察糖尿病肾病(DKD)小鼠足细胞中细胞核苷酸结合寡聚化结构域蛋白2(NOD2)mRNA的表达变化,并探讨其意义。方法将分化完全的小鼠足细胞接种至6孔板,分为DKD组和NC组。DKD组分别加入25、50 mmol/L的膜过滤葡萄糖溶液刺激24 h制备DKD足细胞模型;NC组不给予糖刺激。采用实时荧光定量PCR法检测两组细胞中的NOD2 mRNA。选取上一实验中筛选出的最适浓度葡萄糖刺激足细胞制作DKD模型,分为胞壁酰二肽(MDP)组、shRNA组和模型组。MDP组给予2 g/m L的MDP刺激,shRNA组转染NOD2 shRNA,模型组常规培养,24 h后采用实时荧光定量PCR法检测各组细胞中的NOD2、nephrin、肿瘤坏死因子α(TNF-α)mRNA。结果 DKD组细胞中NOD2 mRNA相对表达量高于NC组,DKD组中50 mmol/L亚组NOD2 mRNA相对表达量高于25 mmol/L亚组(P均0.05)。MDP组细胞中NOD2、TNF-αmRNA相对表达量高于模型组,nephrin mRNA相对表达量低于模型组(P均0.05);shRNA组细胞中NOD2、TNF-αmRNA相对表达量低于模型组,nephrin mRNA相对表达量高于模型组(P均0.05)。结论 DKD小鼠足细胞中NOD2 mRNA表达上调,NOD2 mRNA高表达时足细胞损伤加重,NOD2 mRNA低表达时足细胞损伤减轻;NOD2 mRNA表达变化可能与DKD的发病及病情进展有关。
[Abstract]:Objective to observe the expression of nucleotide-binding oligodeoxyribonucleotide domain protein 2 (NOD2) mRNA in podocytes of diabetic nephropathy (DKD) mice and to explore its significance. Methods the well-differentiated mouse podocytes were inoculated into 6-well plate and divided into two groups: DKD group and NC group. The DKD group was stimulated with 25 ~ 50 mmol / L glucose filtration solution for 24 h to make the model of DKD podocyte without glucose stimulation. NOD2 mRNA in two groups of cells was detected by real-time fluorescence quantitative PCR. The DKD model was made by stimulating podocytes with the optimal concentration of glucose selected in the previous experiment. The cells were divided into two groups: shRNA group and model group. MDP group was treated with 2 g / mL MDP stimulated shRNA group and transfected with NOD2 shRNA. After 24 hours of normal culture, the NOD2 nephrin and tumor necrosis factor 伪 (TNF- 伪) mRNAwere detected by real-time fluorescence quantitative PCR in the model group. Results the relative expression of NOD2 mRNA in the DKD group was higher than that in the 50 mmol / L subgroup of NC group compared with that in the 25 mmol / L subgroup (P 0.05). The relative expression of NOD2 TNF- 伪 mRNA in the MDP group was higher than that in the model group (P < 0.05). The relative expression of NOD2 mRNA in the DKD group was higher than that in the model group (P The relative expression of NOD2TNF- 伪 mRNA in shRNA group was lower than that in model group (P 0.05). Conclusion the up-regulation of NOD2 mRNA expression in podocytes of DKD mice may be related to the pathogenesis and progression of DKD when podocyte injury exacerbates the decrease of NOD2 mRNA expression in podocyte with low NOD2 mRNA expression.
【作者单位】：天津医科大学基础医学院;天津市南开区三潭医院;
【分类号】：R587.2;R692.9

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