辛伐他汀对前列腺上皮细胞RWPE-1增殖及凋亡的影响
发布时间：2018-07-24 12:13
【摘要】：目的:探讨辛伐他汀对前列腺上皮细胞RWPE-1增殖及凋亡的影响。方法:设定不同浓度的辛伐他汀(0、10、20、40μmol/L)分别作用于体外培养的RWPE-1细胞,利用MTT法检测细胞增殖情况,流式细胞术检测细胞的凋亡情况。荧光定量RT-PCR检测RWPE-1细胞的Bcl-2、Bax、Cx43 mRNA的表达,Western印迹检测Bcl-2、Bax、Cx43蛋白的表达。结果:MTT法检测辛伐他汀(10、20、40μmol/L)作用于RWPE-1细胞72 h后,对RWPE-1细胞的抑制率分别为(21.07±6.41)%、(34.87±9.65)%和(47.18±10.88)%,与对照组[(1.21±0.54)%]比较有显著差异(P0.05),并且呈明显的剂量依赖关系(P0.05);处理72 h后各组的凋亡指数分别为:10μmol/L组(0.066±0.016)%,20μmol/L组(0.126±0.023)%,40μmol/L组(0.192±0.025)%,与对照组[(0.015±0.005)%]相比差异显著(P0.01),且呈剂量依赖关系(P0.05)。荧光定量PCR检测显示随着辛伐他汀浓度升高Bcl-2基因表达逐渐下调(P0.05),Bax和Cx43基因表达逐渐上调(P0.05),并且呈剂量依赖关系(P0.05)。Western印迹检测显示RWPE-1细胞内Bcl-2蛋白的表达随辛伐他汀浓度的增高逐渐下调(P0.05),Bax蛋白逐渐上调(P0.05),Cx43蛋白逐渐上调(P0.01),并且皆呈剂量依赖关系(P0.05)。Cx43的表达与Bcl-2的表达呈负相关,与Bax的表达呈正相关。结论:辛伐他汀可能通过影响细胞间隙连接通讯来抑制前列腺上皮细胞增殖并诱导其凋亡。
[Abstract]:Aim: to investigate the effect of simvastatin on RWPE-1 proliferation and apoptosis in prostatic epithelial cells. Methods: different concentrations of simvastatin (0 10 ~ 2040 渭 mol/L) were used to treat RWPE-1 cells in vitro. Cell proliferation was detected by MTT assay and apoptosis was detected by flow cytometry. The expression of Bcl-2AXCx43 mRNA in RWPE-1 cells was detected by fluorescence quantitative RT-PCR. Western blot was used to detect the expression of Bcl-2OBaxCx43 protein. Results Simvastatin (10 ~ 20 渭 mol/L) was applied to RWPE-1 cells for 72 h. The inhibition rates of RWPE-1 cells were (21.07 卤6.41), (34.87 卤9.65)% and (47.18 卤10.88), respectively, which were significantly different from those of the control group [(1.21 卤0.54)%] (P0.05), and the apoptotic index of the control group was (0.066 卤0.016) 渭 mol/L group (0.066 卤0.016), (0.126 卤0.023) 渭 mol/L group (0.192 卤0.025) and (0.015 卤0.005)% compared with the control group [(0.015 卤0.005)%]. The difference was significant (P0.01), and showed a dose-dependent relationship (P0.05). Fluorescence quantitative PCR analysis showed that the expression of Bcl-2 gene decreased gradually (P0.05) and the expression of Cx43 gene increased gradually with the increase of simvastatin concentration (P0.05), and showed a dose-dependent relationship (P0.05) .Western blotting showed that the expression of Bcl-2 protein in RWPE-1 cells was increased with simvastatin. The increase of Tin-level was down-regulated (P0.05). (P0.05) the protein of Cx43 was up-regulated (P0.01), and the expression of Cx43 was negatively correlated with the expression of Bcl-2 in a dose-dependent manner (P0.05). There was a positive correlation with the expression of Bax. Conclusion: simvastatin may inhibit the proliferation and induce apoptosis of prostatic epithelial cells by affecting gap junctional communication.
【作者单位】： 福建医科大学附属漳州市医院泌尿外科;
【基金】：福建省自然科学基金(2012J05164)~~
【分类号】：R697.3
，
本文编号：2141345
[Abstract]:Aim: to investigate the effect of simvastatin on RWPE-1 proliferation and apoptosis in prostatic epithelial cells. Methods: different concentrations of simvastatin (0 10 ~ 2040 渭 mol/L) were used to treat RWPE-1 cells in vitro. Cell proliferation was detected by MTT assay and apoptosis was detected by flow cytometry. The expression of Bcl-2AXCx43 mRNA in RWPE-1 cells was detected by fluorescence quantitative RT-PCR. Western blot was used to detect the expression of Bcl-2OBaxCx43 protein. Results Simvastatin (10 ~ 20 渭 mol/L) was applied to RWPE-1 cells for 72 h. The inhibition rates of RWPE-1 cells were (21.07 卤6.41), (34.87 卤9.65)% and (47.18 卤10.88), respectively, which were significantly different from those of the control group [(1.21 卤0.54)%] (P0.05), and the apoptotic index of the control group was (0.066 卤0.016) 渭 mol/L group (0.066 卤0.016), (0.126 卤0.023) 渭 mol/L group (0.192 卤0.025) and (0.015 卤0.005)% compared with the control group [(0.015 卤0.005)%]. The difference was significant (P0.01), and showed a dose-dependent relationship (P0.05). Fluorescence quantitative PCR analysis showed that the expression of Bcl-2 gene decreased gradually (P0.05) and the expression of Cx43 gene increased gradually with the increase of simvastatin concentration (P0.05), and showed a dose-dependent relationship (P0.05) .Western blotting showed that the expression of Bcl-2 protein in RWPE-1 cells was increased with simvastatin. The increase of Tin-level was down-regulated (P0.05). (P0.05) the protein of Cx43 was up-regulated (P0.01), and the expression of Cx43 was negatively correlated with the expression of Bcl-2 in a dose-dependent manner (P0.05). There was a positive correlation with the expression of Bax. Conclusion: simvastatin may inhibit the proliferation and induce apoptosis of prostatic epithelial cells by affecting gap junctional communication.
【作者单位】： 福建医科大学附属漳州市医院泌尿外科;
【基金】：福建省自然科学基金(2012J05164)~~
【分类号】：R697.3
，
本文编号：2141345
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