间充质干细胞对阿霉素肾病大鼠的保护作用及机制

发布时间：2018-08-29 09:25

【摘要】：目的探讨人脐带间充质干细胞(HUMSCs)移植对阿霉素肾病的保护作用及机制。方法贴壁法培养HUMSCs,SD大鼠随机分为3组:模型组及移植组分别于第1和第8天尾静脉注射4 mg/kg、3.5 mg/kg阿霉素;正常组同期尾静脉注射等量生理盐水;移植组于第1、8、15和22天尾静脉注射HUMSCs(2×10~6个),而模型组及对照组尾静脉注射相同剂量的DMEM。代谢笼留24 h尿测蛋白定量。ELISA法检测血清炎症因子;Real timePCR检测肾组织nephrin和podocin mRNA表达情况。结果 HUMSCs移植后能改善阿霉素肾病的低白蛋白血症和高脂血症,并降低尿蛋白和肾组织nephrin和podocin mRNA表达,血清中白细胞介素-10水平上升,而白细胞介素-6、肿瘤坏死因子-α和前列腺素E2水平则明显下降。结论 HUMSCs移植可以改善大鼠阿霉素肾病的临床及病理,其机制可能是通过调节炎症介质起作用。
[Abstract]:Objective to investigate the protective effect and mechanism of human umbilical cord mesenchymal stem cell (HUMSCs) transplantation on adriamycin nephropathy. Methods HUMSCs,SD rats cultured by adherent method were randomly divided into three groups: the model group and the transplantation group were injected with adriamycin for 4 mg/kg,3.5 mg/kg at the first and eighth day respectively, and the normal group were injected with the same amount of normal saline at the same time. HUMSCs (2 脳 10 ~ 6) was injected into the tail vein of the transplantation group on the 15th and 22nd day after transplantation, while the same dose of DMEM. was injected into the model group and the control group. The expression of nephrin and podocin mRNA in renal tissue was detected by Elisa. Real timePCR was used to detect serum inflammatory factor. Results HUMSCs could improve the hypoalbuminemia and hyperlipidemia of adriamycin nephropathy, decrease the expression of nephrin and podocin mRNA in urine and renal tissue, and increase the level of interleukin-10 in serum. The levels of interleukin-6, tumor necrosis factor-伪 and prostaglandin E _ 2 were significantly decreased. Conclusion HUMSCs transplantation can improve the clinicopathology of adriamycin nephropathy in rats, and its mechanism may be by regulating inflammatory mediators.
【作者单位】：暨南大学第二临床医学院广东省深圳市人民医院肾内科;暨南大学第二临床医学院广东省深圳市人民医院血液科;
【基金】：广东省深圳市卫生计生科研项目(编号:201402005)
【分类号】：R692

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