肾茶及迷迭香酸对三聚氰胺和三聚氰酸联合致小鼠肾结石的作用

发布时间：2018-10-13 11:01

【摘要】：目的建立一种肾功能无异常的三聚氰胺肾结石模型，探讨肾茶及迷迭香酸对泌尿生殖系统可能的保护作用。方法采用两种造模方法实验：①将50只ICR雄性小鼠随机分为正常组、模型组（隔天染毒）、迷迭香酸低剂量组、迷迭香酸高剂量组、友来特组（隔天染毒），上午正常组予NS，其余各组予Mel+Cya，下午各组分别予NS、NS、低剂量RA、高剂量RA、友来特，隔天染毒，30天后处死；②将40只ICR雄性小鼠随机分为模型组（连续染毒）、迷迭香酸组、肾茶组、友来特组（连续染毒），上午各组予Mel+Cya，，下午分别予NS、RA、肾茶、友来特，连续灌胃15天。评价各组小鼠的一般情况、体质量、饮水量，检测血清Cr、BUN、UA、ALT、AST、MDA、SOD，观察肾脏和睾丸的病变，检测肾脏中三聚氰胺的含量。结果隔天染毒30天后，各组小鼠体质量、血生化和氧化指标统计学无差异（P＞0.05）；肾脏病理：模型组（隔天染毒）肾小管腔内可见中等量黄色物质沉积，偶见结晶形成，友来特组（隔天染毒）肾小管腔内未见明显沉积物；睾丸病理：模型组（隔天染毒）睾丸精子细胞和成熟精子数目明显减少，友来特组（隔天染毒）基本正常；肾脏三聚氰胺含量比较：迷迭香酸低剂量组＞模型组（隔天染毒）＞迷迭香酸高剂量组＞友来特组（隔天染毒）＞正常对照组。Mel+Cya连续染毒15天，肾茶组和友来特组（连续染毒）小鼠体重增长较快、饮水量较多；各组小鼠肝肾功能正常，统计学无差异（P＞0.05）；肾脏病理：模型组（连续染毒）肾小管腔内可见大量晶体聚集，肾茶治疗组肾小管内偶见少许沉积物，未见结晶形成，友来特组（连续染毒）肾小管内可见沉积物，偶见结晶；睾丸病理：模型组（连续染毒）睾丸生精细胞排列疏松，成熟精子和间质细胞减少，肾茶治疗组和友来特组（连续染毒）小鼠睾丸组织形态完整；各组肾脏三聚氰胺含量比较：迷迭香酸组＞模型组（连续染毒）＞友来特组（连续染毒）＞肾茶治疗组。结论肾茶对三聚氰胺导致的肾结石具有明显的防治作用；同时能够减轻三聚氰胺及其同系物对睾丸精子生长的抑制作用；迷迭香酸可能会减轻三聚氰胺导致的肾损伤。
[Abstract]:Objective to establish a melamine nephrolithiasis model without abnormal renal function and to explore the protective effect of kidney tea and rosemary acid on urinary and reproductive system. Methods two kinds of modeling methods were used: (1) 50 male ICR mice were randomly divided into normal group, model group, rosemary acid low dose group and rosemary acid high dose group. Eurite group (next day) and normal group (morning group) were given Mel Cya, for the rest of the morning. In the afternoon, each group was given NS, low dose RA, high dose RA,. The next day, the mice were killed 30 days later. 2Forty ICR male mice were randomly divided into three groups: model group (continuous exposure), rosemary acid group, kidney tea group and eurite group (continuous exposure). In the morning, each group was given NS,RA, kidney tea in the afternoon, eurite, continuous intragastric administration for 15 days. The general condition, body weight, drinking quantity, serum Cr,BUN,UA,ALT,AST,MDA,SOD, and the contents of melamine in kidney and testis were evaluated. Results 30 days after exposure, there was no significant difference in body weight, blood biochemistry and oxidation index in each group (P > 0.05). Testicular pathology: the number of testicular spermatozoa and mature spermatozoa decreased significantly in the model group (the next day), and the number of spermatozoa in the eurite group (exposed to poison the next day) was basically normal. Comparison of renal melamine content: rosemary acid low dose group > model group (next day exposure) > rosemary acid high dose group > eurite group (next day exposure) > normal control group. Mel Cya exposure for 15 days. The weight of mice in the kidney tea group and the eurite group (continuous exposure) increased rapidly, and the mice drank more water, the liver and kidney function of each group were normal, there was no statistical difference between the two groups (P > 0.05), and there was no significant difference between the two groups (P > 0.05). Renal pathology: in the model group (continuous exposure), a large number of crystal aggregation could be seen in the renal tubule lumen, a little sediment in the renal tubule was occasionally seen in the kidney tea treatment group, and no crystallization was found in the renal tubules, while in the eurite group (continuous exposure), the deposits in the renal tubules were visible and the crystals were occasionally seen. Testicular pathology: testicular spermatogenic cells were loosely arranged, mature spermatozoa and interstitial cells were decreased in model group (continuous exposure), and testicular tissue was intact in kidney tea treatment group and eurite group (continuous exposure group). Comparison of the contents of melamine in kidney: rosmarinic acid group > model group (continuous exposure) > eurite group (continuous exposure) > kidney tea treatment group. Conclusion Shencha has obvious preventive and therapeutic effects on renal stones induced by melamine and can reduce the inhibitory effect of melamine and its congeners on testicular spermatozoa growth. Rosmarinic acid may alleviate renal injury induced by melamine.
【学位授予单位】：福建医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R692.4

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