荧光差异双向凝胶电泳筛选肾透明细胞癌及癌旁组织中的差异表达蛋白
发布时间：2018-10-16 12:58
【摘要】：目的寻找肾癌相关肿瘤标记物,用于肾癌临床诊断、药物治疗靶点的选择及预后监测。方法采用荧光差异双向凝胶电泳技术筛选15例肾透明细胞癌及癌旁正常肾组织差异表达蛋白质点。统计学采用t检验进行定量比较,以两组蛋白含量比值在1.5倍及以上,P0.05,被认为是差异表达蛋白质点。用基质辅助激光解吸电离飞行时间质谱或串联质谱技术进行差异表达蛋白质点鉴定。结果共筛选分离出27个差异表达蛋白质点,经质谱技术共成功鉴定了26种蛋白质,11种蛋白质在肾癌组织中上调,15种蛋白质下调。其功能覆盖细胞活动的多个方面,其中线粒体短/支链特异性酰基辅酶A脱氢酶、醛糖1-表异构酶、PRDX4、CAPG、β-防御素107、微纤丝相关糖蛋白4等6种蛋白是我们首次采用蛋白质组学技术成功筛选的肾癌差异表达蛋白质,并发现了1个预测蛋白(UCRIL)。结论荧光差异双向凝胶电泳在肾癌组织中获得较好的筛选效果,新筛选的差异表达蛋白有望成为肾癌分子生物学标记物,为后续研究提供了实验依据。
[Abstract]:Objective to search for tumor markers associated with renal cell carcinoma (RCC) for clinical diagnosis, drug therapy target selection and prognosis monitoring. Methods fluorescent differential two dimensional gel electrophoresis was used to screen differentially expressed protein spots in 15 cases of renal clear cell carcinoma and adjacent normal renal tissues. T test was used to make quantitative comparison. The ratio of two groups of protein content was 1.5 times and above, P0.05, which was considered to be a differentially expressed protein spot. Differential expression protein spots were identified by matrix assisted laser desorption ionization time of flight mass spectrometry or tandem mass spectrometry. Results A total of 27 differentially expressed protein spots were isolated and 26 proteins were successfully identified by mass spectrometry. 11 proteins were up-regulated in renal cell carcinoma and 15 proteins were down-regulated. Its function covers many aspects of cell activity, including mitochondrial short / branched-chain specific acylCoA dehydrogenase. Aldose 1-epiisomerase, PRDX4,CAPG, 尾 -defensin 107 and microfibril associated glycoprotein 4 are the first differentially expressed proteins in renal cell carcinoma that we successfully screened by proteomics, and a predictive protein (UCRIL). Was found. Conclusion fluorescence differential two-dimensional gel electrophoresis has a good screening effect in renal cell carcinoma tissues. The newly screened differentially expressed proteins are expected to become molecular biological markers of renal cell carcinoma and provide experimental basis for further study.
【作者单位】： 南方医科大学南方医院泌尿外科;广州医学院附属武警医院泌尿外科;
【基金】：总后勤部“重中之重”学科建设专项基金 南方医科大学南方医院院长基金资助项目(2015C012)
【分类号】：R737.11
[Abstract]:Objective to search for tumor markers associated with renal cell carcinoma (RCC) for clinical diagnosis, drug therapy target selection and prognosis monitoring. Methods fluorescent differential two dimensional gel electrophoresis was used to screen differentially expressed protein spots in 15 cases of renal clear cell carcinoma and adjacent normal renal tissues. T test was used to make quantitative comparison. The ratio of two groups of protein content was 1.5 times and above, P0.05, which was considered to be a differentially expressed protein spot. Differential expression protein spots were identified by matrix assisted laser desorption ionization time of flight mass spectrometry or tandem mass spectrometry. Results A total of 27 differentially expressed protein spots were isolated and 26 proteins were successfully identified by mass spectrometry. 11 proteins were up-regulated in renal cell carcinoma and 15 proteins were down-regulated. Its function covers many aspects of cell activity, including mitochondrial short / branched-chain specific acylCoA dehydrogenase. Aldose 1-epiisomerase, PRDX4,CAPG, 尾 -defensin 107 and microfibril associated glycoprotein 4 are the first differentially expressed proteins in renal cell carcinoma that we successfully screened by proteomics, and a predictive protein (UCRIL). Was found. Conclusion fluorescence differential two-dimensional gel electrophoresis has a good screening effect in renal cell carcinoma tissues. The newly screened differentially expressed proteins are expected to become molecular biological markers of renal cell carcinoma and provide experimental basis for further study.
【作者单位】： 南方医科大学南方医院泌尿外科;广州医学院附属武警医院泌尿外科;
【基金】：总后勤部“重中之重”学科建设专项基金 南方医科大学南方医院院长基金资助项目(2015C012)
【分类号】：R737.11
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