Alport综合征iPSCs差异性表达新小核糖核苷酸的分析

发布时间：2018-10-24 07:18

【摘要】：目的构建Alport综合征(AS)与正常对照者(NC)诱导多能干细胞(i PSCs)新核糖核苷酸(novel microRNA)差异性表达谱,分析其靶基因功能。方法采用前期工作成功从尿肾状杆细胞诱导成的i PSCs,运用高通量测序平台获得AS与NC的差异性表达谱,使用靶基因预测软件Target Scan进行靶基因预测,靶基因与参考基因比较后,在候选靶基因找到显著富集的GO条目及KEGG通路。结果在AS与NC中发现49个有意义的差异性表达novel microRNAs,其中33个表达上调,16个表达下调。在GO靶基因富集分析中,靶基因主要富集于生物调节、生物新陈代谢、细胞组成、细胞信号传导、酶催化反应、分子转运等过程。在KEGG通路分析中,靶基因主要参与代谢通路、嘌呤代谢、癌症转录调节等过程。结论来源于AS与NC的i PSCs存在较大的差异性表达novel microRNA,其靶基因在分子功能、细胞组成、生物过程起着重要作用。这些差异性表达的novel mciroRNAs与靶基因可能是AS发病机制的潜在位点。
[Abstract]:Objective to construct the differentially expressed (novel microRNA) profile of (i PSCs) new ribonucleotide in multipotent stem cells induced by (NC) in Alport syndrome (AS) and normal controls, and to analyze its target gene function. Methods the differentially expressed profiles of AS and NC were obtained by using high-throughput sequencing platform and the target gene prediction software (Target Scan) was used to predict the target gene, and the target gene was compared with the reference gene. Significant enrichment of GO entries and KEGG pathways were found in candidate target genes. Results 49 differentially expressed novel microRNAs, were found in AS and NC, of which 33 were up-regulated and 16 down-regulated. In the enrichment analysis of GO target genes, the target genes are mainly concentrated in the processes of biological regulation, biological metabolism, cell composition, cell signal transduction, enzymatic catalytic reaction, molecular transport and so on. In the analysis of KEGG pathway, target genes are involved in metabolic pathway, purine metabolism and cancer transcriptional regulation. Conclusion I PSCs derived from AS and NC has a great difference in the expression of novel microRNA,. The target gene plays an important role in molecular function, cell composition and biological process. These differentially expressed novel mciroRNAs and target genes may be potential sites for the pathogenesis of AS.
【作者单位】：暨南大学第二临床学院;深圳市人民医院临床医学研究中心;
【基金】：广东省深圳市科技计划(JXY20140416122812045)
【分类号】：R692.3

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