解脲脲原体血清型1和4在雌性小鼠生殖道中的致病性研究

发布时间：2018-10-25 07:36

【摘要】：目的：探讨解脲脲原体(Ureaplasma urealytium, Uu)血清型1(Uu1)和4(Uu4)在雌性小鼠生殖道中的致病性。方法：将408只雌激素预处理过的小鼠随机分为Uu1组和Uu4组,实验组小鼠阴道分别接种三种浓度不同的Uu标准株菌液。对照组小鼠接种无菌培养基。接种后分别于第1、3、7、14、28、42天随机取实验组10只、对照组4只将其处死后取小鼠阴道及宫颈分泌物做荧光定量聚合酶连反应(FQ-PCR)检测解脲脲原体的浓度,并取部分子宫体、输卵管、卵巢组织置于液体培养基检测Uu。取阴道、宫颈、子宫及输卵管、卵巢组织行HE染色进行组织病理学观察。结果：(1)阴道和宫颈分泌物：Uu1及Uu4三组之间总阳性率的差异均有统计学意义(P0.01)。Uu1和Uu4组同种浓度三组之间总阳性率的差异均无统计学意义(P0.05)(2)至观测结束时,Uu1及Uu4三组之间小鼠阴道及宫颈分泌物Uu浓度,各组各时间点浓度比较差异均有统计学意义(P0.05)。(3)Uu组织培养显示Uu1、Uu4组子宫体、输卵管Uu培养阳性率差异均有统计学意义(P0.05)。(4)接种后3-42d,Uu1、Uu4组宫颈总致病率的差异有统计学意义(P0.05)。接种后7-42d, Uu1、Uu4组子宫体与输卵管致病率的差异均有统计学意义(P0.05)。结论：(1)Uu1和Uu4主要在宫颈致病,部分上行可导致上生殖道感染,在阴道可致短期炎症。(2)Uu4的在宫颈和子宫、输卵管的致病性比Uu1强且致病力随Uu感染的浓度增大而增强。
[Abstract]:Objective: to investigate the pathogenicity of Ureaplasma Urealyticum (Ureaplasma urealytium, Uu) serotypes 1 (Uu1) and 4 (Uu4) in female mice reproductive tract. Methods: 408 mice pretreated with estrogen were randomly divided into Uu1 group and Uu4 group. The mice in the experimental group were inoculated with three kinds of Uu standard strains of different concentrations in vagina. The control group mice were inoculated with aseptic medium. After inoculation, 10 mice in the experimental group were randomly selected on the 1st day after inoculation, and 4 mice in the control group were killed. The vaginal and cervical secretions of the mice were taken for fluorescence quantitative polymerase chain reaction (FQ-PCR) to detect the concentration of Ureaplasma Urealyticum, and some uterine bodies and fallopian tubes were taken out. Detection of Uu. in Ovarian tissue on liquid medium The tissues of vagina, cervix, uterus and oviduct were stained with HE for histopathological observation. Results: (1) vaginal and cervical secretions: there was significant difference in total positive rate between Uu1 and Uu4 groups (P0.01). There was no significant difference in total positive rate between Uu1 and Uu4 groups at the same concentration (P0.05) (2), Uu1 and Uu4 at the end of observation. The concentration of Uu in vaginal and cervical secretions of mice between the three groups, There were significant differences in the concentration at each time point in each group (P0.05). (3) Uu tissue culture showed that the positive rate of uterine body and tubal Uu culture in Uu1,Uu4 group were significantly different (P0.05). (4) the total pathogenicity of cervix of 3 ~ 42 days UU1 UU4 group was significantly different (P0.05). From 7 to 42 days after inoculation, the pathogenicity of uterine body and fallopian tube in Uu1,Uu4 group were significantly different (P0.05). Conclusion: (1) Uu1 and Uu4 are mainly pathogenic in the cervix, partly uplink can lead to upper genital tract infection, and in vagina can cause short-term inflammation. (2) the pathogenicity of Uu4 in cervix and uterus is stronger than that of Uu1, and the pathogenicity increases with the increase of Uu infection concentration.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R691.3

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