Y-干扰素在肾间质纤维化中对大鼠肾成纤维细胞的作用研究
发布时间：2018-10-25 17:59
【摘要】：目的：在体内与体外研究中,IFN-y抗肝纤维化,肺损伤纤维化已经得到证实。本研究通过评估IFN-γ对体外培养的大鼠肾成纤维细胞增殖和MCP-1表达的影响,旨在探讨IFN-γ治疗肾间质纤维化的可能机制。方法：体外培养大鼠肾成纤维细胞NRK-49F,分别设立对照组和浓度为1000U/ml、2000U/ml、3000U/ml、4000U/ml、5000U/ml的IFN-y药物干预组,对照组使用0.9%生理盐水。采用MTT比色法检测NRK-49F细胞的增殖情况；RT-PCR和Western-blot方法检测NRK-49F细胞MCP-1 mRNA和MCP-1蛋白水平。结果：1.肾成纤维细胞增殖情况：与对照组相比,采用单因素方差分析,不同浓度IFN-γ组测的OD值均高于对照组(p0.05)；IFN-γ促进NRK-49F细胞增殖呈时间依赖性(p0.05)；不同IFN-y浓度间测在490nm波长的OD值差异不具统计学意义(p0.05)。2. MCP-1 mRNA表达：与对照组相比,IFN-y干预组的MCP-1 mRNA表达水平均明显升高(p0.05)：不同浓度IFN-y间的MCP-1mRNA表达水平无显著性差异(P0.05)。3.MCP-1蛋白表达：与对照组相比,IFN-γ干预组的MCP-1蛋白表达水平均明显升高(p0.05)；不同浓度IFN-γ间的MCP-1蛋白水平无显著性差异(P0.05)。结论：IFN-γ呈时间依赖性的促进NRK-49F细胞的增殖。在基因水平上,IFN-γ能上调NRK-49F细胞MCP-1 mRNA表达水平；在蛋白水平上,IFN-γ能促进NRK-49F细胞分泌MCP-1蛋白。IFN-γ可能通过上调MCP-1参与肾间质纤维化过程。
[Abstract]:Objective: in vivo and in vitro studies, IFN-y has been proved to inhibit liver fibrosis and lung injury fibrosis. The aim of this study was to evaluate the effect of IFN- 纬 on proliferation and MCP-1 expression of rat renal fibroblasts in vitro, and to explore the possible mechanism of IFN- 纬 in the treatment of renal interstitial fibrosis. Methods: in vitro cultured rat renal fibroblasts (NRK-49F,) were divided into two groups: the control group and the control group with the concentration of 1000U / ml 2000U / ml and the control group treated with 0.9% normal saline. The proliferation of NRK-49F cells was detected by MTT colorimetry, and the MCP-1 mRNA and MCP-1 protein levels of NRK-49F cells were detected by RT-PCR and Western-blot methods. The result is 1: 1. Proliferation of renal fibroblasts: compared with the control group, the OD values of IFN- 纬 groups with different concentrations were higher than those of the control group (p0.05), and IFN- 纬 promoted the proliferation of NRK-49F cells in a time-dependent manner (p0.05). There was no significant difference in OD value between different IFN-y concentrations at 490nm wavelength (p0.05). 2. MCP-1 mRNA expression: compared with the control group, the MCP-1 mRNA expression level in the IFN-y intervention group was significantly higher than that in the control group (p0.05). There was no significant difference in the MCP-1mRNA expression level between the different concentrations of IFN-y (P0.05). 3.MCP-1 protein expression: compared with the control group, IFN- 纬 intervention group MCP-1 protein expression water The average increased significantly (p0.05). There was no significant difference in MCP-1 protein level between different concentrations of IFN- 纬 (P0.05). Conclusion: IFN- 纬 promotes the proliferation of NRK-49F cells in a time dependent manner. At the gene level, IFN- 纬 can up-regulate the expression of MCP-1 mRNA in NRK-49F cells, and at the protein level, IFN- 纬 can promote the secretion of MCP-1 protein by NRK-49F cells. IFN- 纬 may participate in the process of renal interstitial fibrosis by up-regulating MCP-1.
【学位授予单位】：昆明理工大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R692
本文编号：2294407
[Abstract]:Objective: in vivo and in vitro studies, IFN-y has been proved to inhibit liver fibrosis and lung injury fibrosis. The aim of this study was to evaluate the effect of IFN- 纬 on proliferation and MCP-1 expression of rat renal fibroblasts in vitro, and to explore the possible mechanism of IFN- 纬 in the treatment of renal interstitial fibrosis. Methods: in vitro cultured rat renal fibroblasts (NRK-49F,) were divided into two groups: the control group and the control group with the concentration of 1000U / ml 2000U / ml and the control group treated with 0.9% normal saline. The proliferation of NRK-49F cells was detected by MTT colorimetry, and the MCP-1 mRNA and MCP-1 protein levels of NRK-49F cells were detected by RT-PCR and Western-blot methods. The result is 1: 1. Proliferation of renal fibroblasts: compared with the control group, the OD values of IFN- 纬 groups with different concentrations were higher than those of the control group (p0.05), and IFN- 纬 promoted the proliferation of NRK-49F cells in a time-dependent manner (p0.05). There was no significant difference in OD value between different IFN-y concentrations at 490nm wavelength (p0.05). 2. MCP-1 mRNA expression: compared with the control group, the MCP-1 mRNA expression level in the IFN-y intervention group was significantly higher than that in the control group (p0.05). There was no significant difference in the MCP-1mRNA expression level between the different concentrations of IFN-y (P0.05). 3.MCP-1 protein expression: compared with the control group, IFN- 纬 intervention group MCP-1 protein expression water The average increased significantly (p0.05). There was no significant difference in MCP-1 protein level between different concentrations of IFN- 纬 (P0.05). Conclusion: IFN- 纬 promotes the proliferation of NRK-49F cells in a time dependent manner. At the gene level, IFN- 纬 can up-regulate the expression of MCP-1 mRNA in NRK-49F cells, and at the protein level, IFN- 纬 can promote the secretion of MCP-1 protein by NRK-49F cells. IFN- 纬 may participate in the process of renal interstitial fibrosis by up-regulating MCP-1.
【学位授予单位】：昆明理工大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R692
【参考文献】
相关期刊论文 前9条
1 吴燕燕;IFN-γ在肺损伤纤维化中的研究进展[J];国外医学(儿科学分册);2003年04期
2 高红宇;黄晓丽;易艳;邵菊芳;刘晓城;;苦参素对肾间质纤维化大鼠单核巨噬细胞浸润及MCP-1表达的影响[J];中国组织化学与细胞化学杂志;2007年02期
3 李萍;刘伏友;刘虹;;肾间质纤维化动物实验研究进展及肾间质纤维化的基因治疗[J];国际泌尿系统杂志;2006年02期
4 张晓猛;刘超;夏光云;;Th1/Th2型细胞因子在小鼠肺纤维化发病中的作用[J];蚌埠医学院学报;2013年08期
5 陈德华;李添应;;干扰素-γ诱导的单核因子、单核细胞趋化蛋白-1和转化生长因子-β1在变应性鼻炎的表达及其意义[J];中华临床免疫和变态反应杂志;2010年02期
6 刘海林,陆汉明,李定国,蒋祖明;α、γ-干扰素对人成纤维细胞的生长和胶原合成的影响[J];上海第二医科大学学报;1997年03期
7 张彩;田志刚;;γ干扰素的负向免疫调节作用[J];现代免疫学;2007年02期
8 童葵塘,金梅,钱春宏,张智清;重组人干扰素γ的分子结构及其生物活性[J];中国生物制品学杂志;2002年02期
9 施贵静;;γ-干扰素对肝星状细胞增殖影响研究[J];临床和实验医学杂志;2008年05期
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