包皮弹性的临床研究
发布时间：2018-10-25 14:44
【摘要】：【目的】 通过对包皮弹性的研究，将其结果应用在包皮作修复材料的手术中，指导合理取材，，用材，从而提高手术的成功率。 【方法】 将2012年12月至2013年6月在郴州市第一人民医院泌尿科行包皮环切手术7~30岁病人100例。按年龄分组：7~14y：儿童组（A组36例）；15~18y：青年组（B组32例）；19~30y：成人组（C组32例）。分别从已切下的包皮的内外板上的不同部位（腹侧、背侧、旁侧）取材，依次在不同的方向（横向、纵向）牵拉，测量包皮的伸展度。测量完后使用10%的福尔马林固定保存，制成病理切片。应用免疫组化及弹力纤维染色来检测各包皮切片的弹性纤维、平滑肌含量，并比较各组内间差异。 【结果】 1.分析同组内包皮的内外板上的相同部位在相同牵拉方向下有无弹性差异。对A、B、 C组内分别进行t检验，P值均0.05,不具有统计学意义，认为同组内包皮的内外板上的相同部位在相同牵拉方向下弹性无显著性差异。 2.分析同组内相同内外板上相同部位的包皮在不同牵拉方向下弹性有无差异。对A、B、 C组内分别t检验结果，P值均0.05，具有统计学意义，根据均值大小，可认为同组内包皮的相同部位在横向牵拉上的弹性要好于纵向。 3.分析同组内相同内外板上不同部位的包皮在相同牵拉方向上弹性有无差异。对A、B、C三组分别进行方差分析，P值均0.05，不具有统计学意义，认为在同一年龄组内相同内外板上不同部位的包皮在相同牵拉方向上弹性无显著性差异。 4.分析不同组间包皮弹性有无差异，即：年龄与包皮弹性有无关系。A组与B组，B组与C组，A组与C组间弹性两两进行t检验，P值均0.05，不具有统计学意义，无显著性差异，认为年龄与包皮弹性大小无关。 5.分析包皮的厚度与弹性有无关系。对包皮厚度和弹性数据进行Pearson相关分析，r=0.906，在0.01水平（双侧）上显著相关。认为，包皮的弹性与厚度之间具有直线负相关关系。 6.分析各年龄组间包皮平滑肌含量有无差异。A组结蛋白表达程度4.72±0.66，B组结蛋白表达程度4.07±0.55，C组结蛋白表达程度3.01±0.82。对A、B、C三组进行单因素方差分析，两两比较采用LSD-t检验，P值0.05，具有统计学意义，认为A、B、C组结蛋白含量差异具有显著性。根据均值大小，可认为包皮组织标本上的结蛋白含量（即平滑肌含量）随着年龄增长逐渐减少。 7.分析各年龄组间包皮弹性纤维含量有无差异。对A、B、C三组进行LSD-t检验，P值0.05，具有统计学意义，认为A、B、C组弹性纤维含量差异具有显著性。根据均值大小，可认为包皮组织标本上的弹性纤维含量随着年龄增长逐渐增多。 【结论】 1.包皮在横向的牵拉长度大于纵向。 2.包皮组织越厚其弹性越差，反之亦然。 3.包皮组织标本上的平滑肌含量随着年龄增长逐渐减少。 4.包皮组织标本上的弹性纤维含量随着年龄增长逐渐增多。
[Abstract]:[objective] to study the elasticity of the prepuce and apply the results to the operation of the prepuce as the repair material to guide the reasonable selection and use of the material. [methods] from December 2012 to June 2013, 100 patients aged from 7 to 30 years old underwent circumcision of prepuce in Department of urology, the first people's Hospital of Chenzhou. According to the age group, children group (group A, n = 36), young group (group B, n = 32) and adult group (n = 32, n = 32), group A (n = 36), group B (n = 32), group B (n = 32). The different parts (ventral side, dorsal side, side) of the outer and outer plates of the cut prepuce were taken, and the extensibility of the prepuce was measured in different directions (transverse and longitudinal). After the measurement, 10% formalin was fixed and preserved to make pathological sections. The content of elastic fiber and smooth muscle of each prepuce section was detected by immunohistochemistry and elastic fiber staining, and the difference between each group was compared. [results] 1. To analyze the elastic difference of the same part on the inner and outer plate of the same group in the same pulling direction. T test was carried out in group A B and group C, P values were 0.05, there was no statistical significance. It was concluded that there was no significant difference in elasticity between the same part of the inner and outer plates of the same group under the same pulling direction. 2. The elasticity of the same part of prepuce on the same internal and external plate in the same group was analyzed under different pulling directions. According to the mean value, the elasticity of the same part of the prepuce in the same group was better than that in the longitudinal. The elasticity of different parts of the same internal and external plate in the same group in the same stretch direction was analyzed. The results of ANOVA showed that there was no significant difference in the elasticity of different parts of the prepuce on the same internal and external plate in the same age group in the same pulling direction. 4. There was no significant difference between group A and group B, group B and group C, group A and group C were tested by t test (P = 0.05), there was no significant difference between group A and group B, and there was no significant difference between group A and group B, group B and group C, and there was no significant difference between group A and group B, and there was no significant difference between group A and group B, group B and group C by t test. It is considered that age has nothing to do with the size of prepuce elasticity. 5. The relationship between the thickness of prepuce and elasticity is analyzed. The Pearson correlation analysis of prepuce thickness and elastic data showed that rn was 0.906, which was significantly correlated at 0. 01 level (bilateral). It is concluded that there is a linear negative correlation between the elasticity and thickness of the foreskin. The expression degree of desmin in group A was 4.72 卤0.66mb and the expression level of desmin was 3.01 卤0.82in group C (4.07 卤0.55C). The single factor ANOVA was used in the three groups, and the LSD-t test was used to compare the two groups (P = 0.05). The results showed that there was a significant difference in the content of desmin in group A and B (P = 0.05), and the results showed that there was a significant difference in the content of desmin between the two groups. According to the mean value, the desmin content (that is, smooth muscle content) in prepuce tissue decreased gradually with age. The content of prepuce elastic fiber in different age groups was analyzed. The results of LSD-t test showed that there was a significant difference in elastic fiber content among three groups (P = 0.05). According to the mean value, it can be concluded that the content of elastic fiber in prepuce tissue increases with age. [conclusion] 1. The length of the circumference in the transverse stretch is larger than that in the longitudinal. 2. The thicker the foreskin is, the less elastic it is, and vice versa. The content of smooth muscle in prepuce tissue decreased with age. 4. 4. The content of elastic fibers in prepuce tissue increased with age.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R699.8
本文编号：2293998
[Abstract]:[objective] to study the elasticity of the prepuce and apply the results to the operation of the prepuce as the repair material to guide the reasonable selection and use of the material. [methods] from December 2012 to June 2013, 100 patients aged from 7 to 30 years old underwent circumcision of prepuce in Department of urology, the first people's Hospital of Chenzhou. According to the age group, children group (group A, n = 36), young group (group B, n = 32) and adult group (n = 32, n = 32), group A (n = 36), group B (n = 32), group B (n = 32). The different parts (ventral side, dorsal side, side) of the outer and outer plates of the cut prepuce were taken, and the extensibility of the prepuce was measured in different directions (transverse and longitudinal). After the measurement, 10% formalin was fixed and preserved to make pathological sections. The content of elastic fiber and smooth muscle of each prepuce section was detected by immunohistochemistry and elastic fiber staining, and the difference between each group was compared. [results] 1. To analyze the elastic difference of the same part on the inner and outer plate of the same group in the same pulling direction. T test was carried out in group A B and group C, P values were 0.05, there was no statistical significance. It was concluded that there was no significant difference in elasticity between the same part of the inner and outer plates of the same group under the same pulling direction. 2. The elasticity of the same part of prepuce on the same internal and external plate in the same group was analyzed under different pulling directions. According to the mean value, the elasticity of the same part of the prepuce in the same group was better than that in the longitudinal. The elasticity of different parts of the same internal and external plate in the same group in the same stretch direction was analyzed. The results of ANOVA showed that there was no significant difference in the elasticity of different parts of the prepuce on the same internal and external plate in the same age group in the same pulling direction. 4. There was no significant difference between group A and group B, group B and group C, group A and group C were tested by t test (P = 0.05), there was no significant difference between group A and group B, and there was no significant difference between group A and group B, group B and group C, and there was no significant difference between group A and group B, and there was no significant difference between group A and group B, group B and group C by t test. It is considered that age has nothing to do with the size of prepuce elasticity. 5. The relationship between the thickness of prepuce and elasticity is analyzed. The Pearson correlation analysis of prepuce thickness and elastic data showed that rn was 0.906, which was significantly correlated at 0. 01 level (bilateral). It is concluded that there is a linear negative correlation between the elasticity and thickness of the foreskin. The expression degree of desmin in group A was 4.72 卤0.66mb and the expression level of desmin was 3.01 卤0.82in group C (4.07 卤0.55C). The single factor ANOVA was used in the three groups, and the LSD-t test was used to compare the two groups (P = 0.05). The results showed that there was a significant difference in the content of desmin in group A and B (P = 0.05), and the results showed that there was a significant difference in the content of desmin between the two groups. According to the mean value, the desmin content (that is, smooth muscle content) in prepuce tissue decreased gradually with age. The content of prepuce elastic fiber in different age groups was analyzed. The results of LSD-t test showed that there was a significant difference in elastic fiber content among three groups (P = 0.05). According to the mean value, it can be concluded that the content of elastic fiber in prepuce tissue increases with age. [conclusion] 1. The length of the circumference in the transverse stretch is larger than that in the longitudinal. 2. The thicker the foreskin is, the less elastic it is, and vice versa. The content of smooth muscle in prepuce tissue decreased with age. 4. 4. The content of elastic fibers in prepuce tissue increased with age.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R699.8
【参考文献】
相关期刊论文 前3条
1 余灵祥,邹仲之;主动脉平滑肌细胞在三维培养中产生弹性纤维的研究[J];解剖学报;2004年01期
2 裴宪武;包皮为供区的皮肤移植[J];中国修复重建外科杂志;2003年02期
3 唐耘熳;黄进;陈绍基;黄鲁刚;王明和;;小儿先天性无尿道下裂阴茎下曲畸形的解剖特点及诊治[J];中国修复重建外科杂志;2006年03期
本文编号：2293998
本文链接：https://www.wllwen.com/yixuelunwen/mjlw/2293998.html
最近更新
教材专著
