大鼠肾缺血再灌注损伤后VEGF的表达及川芎嗪的干预作用

发布时间：2018-11-02 08:39

【摘要】：目的:通过检测大鼠肾缺血再灌注损伤模型后不同时段肾功能、血清血管内皮生长因子的表达量及肾脏病理,了解血管内皮生长因子在肾缺血再灌注损伤发生后的功能及川芎嗪的干预作用。方法:制作大鼠肾缺血再灌注损伤模型,采用夹心双抗免疫酶联吸附法测定不同组别血清血管内皮生长因子的表达,与肾脏病理、肾功能的相关指标进行分析。结果:1、缺血再灌注组和川芎嗪组大鼠术后12和24小时血钾、血尿素氮高于同时段的假手术组,且有统计学意义(P0.05)。2、川芎嗪组大鼠血钾、尿素氮均低于同时段缺血再灌注组,且有统计学意义。(P0.05)3、缺血再灌注组、川芎嗪组组内12小时和24小时血钾、尿素氮之间差异有统计学意义。(P0.05)4、缺血再灌注组和川芎嗪组各时段血肌酐平均值均高于同时段假手术组;川芎嗪组血肌酐各时段平均值小于缺血再灌注组,12小时内血肌酐平均值上升小于缺血再灌注组。5、缺血再灌注组、川芎嗪组的大鼠肾组织Paller氏评分都分别明显大于假手术组;川芎嗪组的肾小管Paller氏评分分别小于同时段缺血再灌注组。6、缺血再灌注组和川芎嗪大鼠组术后12小时组血清血管内皮生长因子的表达均高于同时段的假手术组;川芎嗪组大鼠术后12小时血清VEGF表达低于同时段缺血再灌注组。结论:1、脊柱旁开约0.5cm、背双侧肋弓下缘纵行约1.5cm切口夹闭双侧大鼠肾动静脉60分钟后恢复灌注可以成功制作大鼠肾缺血再灌注损伤模型,且此方法模型操作时简便易行,成功率高;2、缺血再灌注组和川芎嗪组术后12小时血清血管内皮生长因子表达量即有升高趋势,血管内皮生长因子可以作为早期肾脏缺血损伤的检验指标;3、川芎嗪可以降低肾缺血再灌注损伤发生后的血钾、肌酐、尿素氮的数值、肾小管损伤评分,并起到保护肾脏结构和功能的作用。
[Abstract]:Objective: to investigate the expression of serum vascular endothelial growth factor (VEGF) and renal pathology in different periods after renal ischemia reperfusion injury in rats. To investigate the function of vascular endothelial growth factor (VEGF) after renal ischemia reperfusion injury and the intervention of ligustrazine. Methods: the rat model of renal ischemia-reperfusion injury was established. The expression of vascular endothelial growth factor (VEGF) in different groups of serum was measured by sandwich double immunosorbent assay (Elisa), and the correlation indexes of renal pathology and renal function were analyzed. Results: 1. The blood potassium and blood urea nitrogen of rats in ischemia reperfusion group and ligustrazine group were significantly higher than those in sham operation group at 12 and 24 hours postoperatively (P0.05). Urea nitrogen was lower than that of ischemia reperfusion group at the same time, and had statistical significance. (P0.05) 3, Ischemia-reperfusion group, Ligustrazine group 12 hours and 24 hours blood potassium, urea nitrogen difference was statistically significant. (P0.05) 4, The mean value of serum creatinine in ischemia-reperfusion group and ligustrazine group was higher than that in sham-operation group at the same time. The mean value of serum creatinine in ligustrazine group was lower than that in ischemia-reperfusion group, and the mean value of creatinine in 12 hours was lower than that in ischemia-reperfusion group. The Paller's score of renal tissue in ligustrazine group was significantly higher than that in sham operation group. The Paller's score of renal tubules in ligustrazine group was lower than that in ischemia-reperfusion group at the same time. 6. The expression of vascular endothelial growth factor (VEGF) in serum of ligustrazine group and ligustrazine group 12 hours after operation was higher than that in sham-operation group at the same time. The expression of serum VEGF in ligustrazine group was lower than that in ischemia reperfusion group 12 hours after operation. Conclusion: 1. The model of renal ischemia-reperfusion injury in rats can be made successfully after 60 minutes of bilateral renal arteriovenous occlusion by longitudinal 1.5cm incision along the lower margin of dorsal bilateral costal arch. The method is simple and easy to operate and has a high success rate. 2. The expression of vascular endothelial growth factor (VEGF) in serum of ischemia reperfusion group and ligustrazine group increased 12 hours after operation. Vascular endothelial growth factor could be used as an index of early renal ischemia injury. 3. Ligustrazine could reduce the values of serum potassium, creatinine, urea nitrogen, renal tubular injury score and protect the renal structure and function after renal ischemia-reperfusion injury.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R692

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