膀胱癌组织GPR48表达及其临床意义

发布时间：2018-11-04 11:08

【摘要】：目的 G蛋白偶联受体48(G protein coupled receptor 48,GPR48)在人类细胞信号传导过程中起重要作用,并且与人类肿瘤的发生相关。本研究旨在探讨膀胱癌组织GPR48的表达水平,分析其与膀胱癌恶性程度的相关性。方法选取2014-01-01-2016-05-20郑州大学附属郑州中心医院泌尿外科住院行手术切除治疗的膀胱癌组织标本60例和癌旁组织(距肿瘤组织2cm)标本8例,BIU-87、5637和T24 3种膀胱癌细胞系作为研究对象,通过RT-PCR和蛋白质印迹法检测GPR48的表达量,收集60例患者性别、年龄、病理分级等临床病理资料,并检测其与GPR48表达的相关性。结果 GPR48在膀胱癌旁组织及癌旁组织中均有表达,膀胱癌组织和癌旁组织GPR48mRNA表达量分别为0.080±0.048和0.041±0.032,差异有统计学意义,t=-3.047,P=0.005。膀胱癌组织和癌旁组织GPR48蛋白表达量分别为0.533±0.307和0.144±0.086,差异有统计学意义,t=-3.541,P=0.001。分析患者临床病理资料发现,GPR48的表达与患者性别无关,P0.05;而与患者年龄、病理分级和临床分期呈正相关,均P0.01。BIU-87、5637和T24细胞系中GPR48 mRNA的表达量分别为0.021±0.002、0.054±0.006和0.145±0.003,GPR48蛋白的表达量分别为0.718±0.076、0.905±0.121和1.205±0.08。T24细胞系GPR48 mRNA及蛋白表达量显著高于5637细胞系,均P0.05;5637细胞系GPR48 mRNA及蛋白表达量显著高于BIU-87细胞系,均P0.05。结论 GPR48表达与膀胱癌的恶性程度密切相关,有望成为一种潜在的生物标志物来预测膀胱癌的预后。
[Abstract]:Objective G-protein-coupled receptor 48 (G protein coupled receptor 48 GPR48 plays an important role in the signal transduction of human cells and is associated with the development of human tumors. The aim of this study was to investigate the expression of GPR48 in bladder cancer and to analyze its correlation with the malignancy of bladder cancer. Methods 60 specimens of bladder cancer and 8 specimens of adjacent tissues (2cm) were selected from the urology department of Zhengzhou Central Hospital affiliated to Zhengzhou University, from January to May 2015-20, 2014-01-2016-20. The specimens were collected from 60 cases of bladder cancer and 8 cases of adjacent tissues (2cm). BIU-87,5637 and T24 bladder cancer cell lines were used to detect the expression of GPR48 by RT-PCR and Western blotting. The clinicopathological data of 60 patients were collected, such as sex, age, pathological grade, and the correlation between GPR48 expression and GPR48 expression. Results the expression of GPR48 was found in paracancerous and paracancerous tissues. The expression of GPR48mRNA was 0.080 卤0.048 and 0.041 卤0.032 in bladder cancer and paracancerous tissues, respectively. The expression of GPR48 protein was 0.533 卤0.307 and 0.144 卤0.086 in bladder cancer and paracancerous tissues, respectively. It was found that the expression of GPR48 was not related to the sex of the patients (P0.05). There was a positive correlation between the expression of GPR48 mRNA and age, pathological grade and clinical stage. The expression of GPR48 mRNA was 0.021 卤0.002, 0.054 卤0.006 and 0.145 卤0.003 in P0.01.BIU-87-5637 and T24 cell lines, respectively. The expression of GPR48 protein was 0.718 卤0.076, 0.905 卤0.121 and 1.205 卤0.08.T24, respectively, which was significantly higher than that of 5637 cell line (P 0.05). The expression of GPR48 mRNA and protein in 5637 cell line was significantly higher than that in BIU-87 cell line (P 0.05). Conclusion the expression of GPR48 is closely related to the malignancy of bladder cancer, which may be a potential biomarker to predict the prognosis of bladder cancer.
【作者单位】：郑州大学附属郑州中心医院泌尿外科;
【分类号】：R737.14

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