含碘造影剂对人肾小管上皮细胞坏死的诱导作用

发布时间：2018-11-11 08:40

【摘要】：目的研究含碘造影剂是否能够导致肾小管上皮细胞坏死。方法体外培养人肾小管上皮细胞,分为对照组、碘海醇组(75 mg I·m L~(-1))、泛影葡胺组(75 mg I·m L~(-1))。以碘海醇和泛影葡胺干预细胞2 h以及药物移除5,22 h后,用噻唑蓝(MTT)法测定不同时间点细胞活力,以乳酸脱氢酶试剂盒测定细胞上清液乳酸脱氢酶(LDH)释放;用高内涵成像系统对Hoechst33342/PI双染结果进行定量分析,计算细胞凋亡率和死亡率。结果与对照组相比,碘海醇和泛影葡胺干预2 h后导致细胞活力降低,分别为(52.98±6.75)%,(47.16±4.18)%,差异有统计学意义(P0.01);同时,还导致LDH释放增加分别为(201.31±11.69),(256.21±19.82)U·L~(-1),差异有统计学意义(P0.01),且药物移除后5,22 h时这种影响仍存在。碘海醇组和泛影葡胺组的细胞凋亡率分别为(23.09±4.25)%,(38.64±8.32)%,细胞坏死率分别为(19.69±2.13)%,(24.07±6.18)%,与对照组相比,差异有统计学意义(P0.01);泛影葡胺导致细胞坏死更显著,差异有统计学意义(P0.05)。结论含碘造影剂能够诱导人肾小管上皮细胞坏死。
[Abstract]:Objective to study whether iodized contrast agent can cause renal tubular epithelial cell necrosis. Methods Human renal tubular epithelial cells were cultured in vitro and divided into control group and 75 mg I m L ~ (-1), meglumine group (75 mg I m L ~ (-1). The cell viability was determined by thiazolyl (MTT) assay and lactate dehydrogenase (LDH) release from supernatant of cells was determined by lactate dehydrogenase kit after 2 h treatment with iodohydrin and meglumine diatrizoate and 522 h after drug removal. The Hoechst33342/PI double staining results were quantitatively analyzed by high content imaging system, and the apoptosis rate and death rate were calculated. Results compared with the control group, the cell viability was decreased after 2 hours of treatment with iodohexol and meglumine diatrizoate (52.98 卤6.75)% and (47.16 卤4.18)%, respectively (P0.01). At the same time, the LDH release was increased by (201.31 卤11.69), (卤19.82) U L ~ (-1), the difference was statistically significant (P0.01), and the effect was still present at 5h after drug removal. The apoptotic rate and necrosis rate were (23.09 卤4.25)%, (38.64 卤8.32)%, (19.69 卤2.13)% and (24.07 卤6.18)%, respectively, in iodohexol group and meglumine diatrizoate group. The difference was statistically significant (P0.01). Diatrizoate meglumine caused more significant cell necrosis, the difference was statistically significant (P0.05). Conclusion Iodine-containing contrast agent can induce the necrosis of human renal tubular epithelial cells.
【作者单位】：中南大学湘雅三医院药学部;
【基金】：教育部新世纪优秀人才支持计划基金资助项目(NCET-13-0605) 中南大学研究生自主探索创新基金资助项目(2016zzts569)
【分类号】：R692

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