大鼠精索静脉曲张模型及其高位结扎术后睾丸生精细胞凋亡及其IL-1和NO含量的变化

发布时间：2018-11-24 18:32

【摘要】：目的:研究大鼠左侧精索静脉曲张(VC)模型及其高位结扎术后睾丸生精细胞凋亡及白细胞介素-1(IL-1)和一氧化氮(NO)含量的变化。方法:选用雄性SD大鼠60只,均选择左侧精索静脉作为研究对象,建立VC模型。将大鼠随机分为3组:假手术组(SO)15只,VC后高位结扎组(VCT)组15只和VC模型对照组30只。模型对照组中随机选取15只大鼠作为VC1组,余下15只大鼠作为VC2组,分别测定VC1组和SO组、VC2组和VCT组大鼠精液质量及睾丸组织中IL-1和NO的含量并加以比较,采用TUNEL检测睾丸生精细胞凋亡情况。结果:所有大鼠均建模成功,VC1组精子浓度[(1.54±1.16)×10~6/ml]和精子活力[(44.23±15.46)%]均显著低于SO组[2.80±1.62)×10~6/ml、(72.34±12.62)%](P0.05),VCT组精子浓度[1.82±1.34)×10~6/ml]和精子活力[(51.21±12.62)%]较VC2组有显著提高[(1.04±1.21)×10~6/ml、(39.23±13.21)%](P0.05)。大鼠左侧睾丸NO[(0.172±0.030)ng/ml]、IL-1[(1.468±0.080)mg/ml]含量VC1组明显高于SO组[(0.134±0.021)ng/ml、(0.782±0.079)mg/ml](P0.05),VC2组左侧睾丸NO[(0.198±0.020)ng/ml]、IL-1[(1.994±0.090)mg/ml]含量明显高于VCT组[(0.141±0.010)ng/ml、(0.781±0.036)mg/ml](P0.05),而右侧睾丸2组比较差异无显著性(P0.05),而且NO与IL-1含量之间呈正相关关系(r=0.492,P0.01)。VC1组大鼠双侧睾丸生精细胞大量凋亡,左、右侧睾丸生精细胞凋亡指数差异有统计学意义(P0.05),SO组左、右侧睾丸生精细胞凋亡指数无明显差异(P0.05),2组间同侧睾丸生精细胞凋亡指数差异显著,均有统计学意义(P0.01);VCT组大鼠左、右侧睾丸生精细胞凋亡指数差异有统计学意义(P0.05);VC2组左、右侧睾丸生精细胞凋亡指数无明显差异(P0.05);2组间同侧睾丸生精细胞凋亡指数差异显著,均有统计学意义(P0.01)。VC2组、VCT组组内同侧睾丸生精细胞凋亡指数无明显差异(P0.05),但VC2组、VCT组2组间同侧睾丸生精细胞凋亡指数差异显著,均有统计学意义(P0.01)。结论:VC致睾丸组织中NO和IL-1含量升高,并加重睾丸生精细胞凋亡,可能是其致睾丸损伤、影响睾丸生精功能障碍的原因之一。
[Abstract]:Aim: to study the changes of testicular spermatogenic cell apoptosis and the contents of interleukin-1 (IL-1) and nitric oxide (no) (NO) in the left varicocele (VC) model of rats after high ligation. Methods: 60 male SD rats were selected and the left spermatic vein was selected as the study object to establish VC model. The rats were randomly divided into three groups: sham operation group (SO) (n = 15), VC post high ligation group (VCT) (n = 15) and VC model control group (n = 30). In the model control group, 15 rats were randomly selected as VC1 group and the remaining 15 rats as VC2 group. The semen quality and the contents of IL-1 and NO in testis of VC1 group and SO group, VC2 group and VCT group were measured and compared respectively. Apoptosis of testicular spermatogenic cells was detected by TUNEL. Results: the sperm concentration and sperm motility in VC1 group [(1.54 卤1.16) 脳 10~6/ml] and sperm motility [(44.23 卤15.46)%] were significantly lower than those in SO group [2.80 卤1.62) 脳 10 ~ (-6) / ml, respectively. (72.34 卤12.62)%] (P0.05) sperm concentration [1.82 卤1.34) 脳 10~6/ml] and sperm motility [(51.21 卤12.62)%] in), VCT group were significantly higher than those in VC2 group [(1.04 卤1.21) 脳 10 ~ (-6) / ml]. (39.23 卤13.21)%] (P0.05). The contents of NO [(0.172 卤0.030) ng/ml] and IL-1 [(1.468 卤0.080) mg/ml] in left testis of rats in VC1 group were significantly higher than those in SO group [(0.134 卤0.021) ng/ml, (0.782 卤0.079) mg/ml] (P0.05). The contents of NO [(0.198 卤0.020) ng/ml] and IL-1 [(1.994 卤0.090) mg/ml] in left testis of VC2 group were significantly higher than those in VCT group [(0.141 卤0.010) ng/ml, (0.781 卤0.036) mg/ml] (P0.05). However, there was no significant difference between the two groups in the right testis (P0.05), and there was a positive correlation between the content of NO and IL-1 (r = 0.492, P0.01). The number of spermatogenic cells in the testis of the VC1 group was significantly higher than that in the control group (P < 0.05). The apoptosis index of testicular spermatogenic cells in the right testis was significantly different (P0.05) between the), SO group and the right side group (P0.05), but there was significant difference between the two groups in the ipsilateral testicular spermatogenic cell apoptosis index (P0.05). All had statistical significance (P0.01). The apoptosis index of spermatogenic cells in the left and right testis of VCT group was significantly different (P0.05), while that of the left and right testicular spermatogenic cells in VC2 group had no significant difference (P0.05). The apoptotic index of testicular spermatogenic cells in VC2 group and VCT group had no significant difference (P0.05), but in VC2 group, there was no significant difference between the two groups (P0.01), but there was no significant difference between the two groups in the apoptosis index of testicular spermatogenic cells (P0.01). The apoptosis index of testicular spermatogenic cells in VCT group was significantly different (P0.01). Conclusion: VC can increase the contents of NO and IL-1 in testis and aggravate the apoptosis of testicular spermatogenic cells, which may be one of the causes of testicular injury and the dysfunction of testicular spermatogenesis.
【作者单位】：南京医科大学鼓楼临床医学院男科;南京军区南京总医院泌尿外科;
【基金】：国家自然科学基金资助(81270694) 南京军区南京总医院立项课题(2015011)~~
【分类号】：R697.24

【相似文献】