IL-17和IL-8在单纯BPH与合并炎症BPH患者前列腺组织中的表达

发布时间：2018-12-10 20:22

【摘要】：目的:探讨单纯良性前列腺增生(BPH)与伴有前列腺炎的BPH患者前列腺组织中IL-17和IL-8表达差异。方法:收集行经尿道前列腺电切术的60例BPH患者标本。根据标本HE染色不同分为单纯BPH组23例和合并炎症的BPH组37例。对两组患者的临床资料进行分析比较;用免疫组化方法检测各标本IL-17和IL-8的表达情况,用荧光定量PCR和Western印迹检测各标本IL-17和IL-8 mRNA和蛋白的表达。结果:单纯BPH组和合并炎症的BPH组患者的国际前列腺症状评分(IPSS)[(21.6±3.7)分vs(29.1±6.2)分]、生活质量评分(Qo L)[(4.4±1.6)分vs(5.4±1.3)分]、最大尿流率(Qmax)[(8.2±1.8)ml/s vs(4.7±2.1)ml/s]、残余尿量(RUV)[(98.2±19.3)ml vs(198.6±15.5)ml]、前列腺体积[(49.8±16.5)ml vs(69.2±24.1)ml]、PSA[(2.8±0.8)μg/L vs(7.4±1.9)μg/L]和C反应蛋白(CRP)[(1.5±0.6)mg/L vs(5.1±2.0)mg/L],两组相比差异明显,有统计学意义(P0.05);合并炎症的BPH组患者尿潴留的发生率[32.4%(12/37)]明显高于单纯BPH组[8.69%(2/23),P0.05];IL-17 mRNA和IL-8 mRNA在合并炎症的BPH组前列腺组织中的表达量(0.303±0.076和0.536±0.059)均明显高于单纯BPH组(0.042±0.019和0.108±0.025,P0.05);IL-17和IL-8蛋白在单纯BPH组和在合并炎症的BPH组患者前列腺组织中的蛋白表达(0.34±0.05 vs 0.88±0.10,0.43±0.04 vs1.07±0.08),两者相比差异显著,有统计学意义(P0.05)。结论:IL-17和IL-8在合并炎症的BPH患者前列腺组织中的表达上调,其可能在BPH的发生发展中起重要作用。
[Abstract]:Objective: to investigate the expression of IL-17 and IL-8 in benign prostatic hyperplasia (BPH) and prostatitis (BPH). Methods: 60 patients with BPH underwent transurethral resection of prostate. According to HE staining, 23 cases of BPH group and 37 cases of BPH group with inflammation were divided into two groups: BPH group (n = 23) and BPH group (n = 37). The clinical data of the two groups were analyzed and compared, the expression of IL-17 and IL-8 was detected by immunohistochemical method, and the expression of IL-17 and IL-8 mRNA and protein was detected by fluorescence quantitative PCR and Western blot. Results: the international prostatic symptom score (IPSS) in BPH group and BPH group with inflammation [(21.6 卤3.7) vs (29.1 卤6.2)]. Quality of life score (Qo L) [(4.4 卤1.6) vs (5.4 卤1.3)], maximum urinary flow rate (Qmax) [(8.2 卤1.8) ml/s vs (4.7 卤2.1) ml/s]. Residual urine volume (RUV) [(98.2 卤19.3) ml vs (198.6 卤15.5) ml], prostate volume [(49.8 卤16.5) ml vs (, 69.2 卤24.1) ml], and prostatic volume [(49.8 卤16.5) ml vs (, 69.2 卤24.1) ml]. PSA [(2.8 卤0.8) 渭 g / L vs (7.4 卤1.9) 渭 g / L] and C-reactive protein (CRP) [(1.5 卤0.6) mg/L vs (5.1 卤2.0) mg/L] were significantly different between the two groups. There was statistical significance (P0.05); The incidence of urinary retention in the BPH group with inflammation [32.4% (12 / 37)] was significantly higher than that in the simple BPH group [8.69% (2 / 23), P0.05]. The expression of IL-17 mRNA and IL-8 mRNA in BPH group with inflammation (0.303 卤0.076 and 0.536 卤0.059) was significantly higher than that in BPH group (0.042 卤0.019 and 0.108 卤0.025). The expression of IL-17 and IL-8 protein in prostatic tissues of patients with BPH alone and BPH with inflammation (0.34 卤0.05 vs 0.88 卤0.10 卤0.43 卤0.04 vs1.07 卤0.08) was significantly different. There was statistical significance (P0.05). Conclusion: the expression of IL-17 and IL-8 in prostatic tissues of BPH patients with inflammation may play an important role in the occurrence and development of BPH.
【作者单位】：福建医科大学附属第一医院泌尿外科;福建医科大学附属第一医院男科;
【基金】：福建省中青年教师教育科研项目(JB13391)~~
【分类号】：R699.8

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