成年男性精子线粒体DNA拷贝数及完整性与精液参数的关联研究

发布时间：2019-01-01 15:40

【摘要】：目的分析成年男性精子线粒体DNA(mitochondrial DNA,mt DNA)拷贝数及完整性与常规精液质量参数的关联,探讨精子mt DNA相关指标在反映男性生育力中的作用。方法采集161例参加男性生殖健康调查的志愿者的精液样本。采用计算机辅助精子分析系统(computer-aided sperm analysis,CASA)进行精液参数分析,Diff-Quick试剂盒染色检测精子形态。实时荧光定量PCR测定精子mt DNA拷贝数,长链PCR检测mt DNA完整性。结果 mt DNA拷贝数与常规精液参数包括精液体积、精子密度、精子总数、前向运动率、精子活率和精子正常形态率均呈负相关(r=-0.168、-0.452、-0.474、-0.189、-0.306、-0.235,P均0.05)。除精子前向运动率外,mt DNA完整性与其余常规精液参数均呈正相关(r=0.193、0.379、0.480、0.325、0.282,P均0.05)。采用多元线性回归分析调整混杂因素后,mt DNA相关指标与精子密度、精子总数、精子活率和正常形态率之间的相关性仍然存在。结论 mt DNA拷贝数和mt DNA完整性与常规精液质量参数之间存在关联,mt DNA相关指标可能是反映精子质量的生物标志物。
[Abstract]:Objective to analyze the correlation between sperm mitochondrial DNA (mitochondrial DNA,mt DNA copy number and sperm integrity and sperm quality parameters in adult male sperm, and to explore the role of sperm mt DNA related indexes in reflecting male fertility. Methods semen samples were collected from 161 volunteers who participated in male reproductive health survey. Semen parameters were analyzed by computer aided sperm analysis system (computer-aided sperm analysis,CASA) and sperm morphology was detected by Diff-Quick kit staining. The copy number of sperm mt DNA was measured by real-time fluorescence quantitative PCR and the integrity of mt DNA was detected by long chain PCR. Results there was a negative correlation between mt DNA copy number and sperm volume, sperm density, total sperm count, forward motility rate, sperm motility rate and normal sperm morphology rate (r = 0.168U -0.452U -0.474U -0.1899). -0.306% -0.235% (P 0.05). There was a positive correlation between the integrity of, mt DNA and the other normal semen parameters except the forward motility rate of spermatozoa (r = 0.193, 0.379, 0.380, 0.325, 0.282, respectively, P 0.05). The correlation between, mt DNA and sperm density, total sperm count, sperm motility and normal morphology was still observed after adjusting confounding factors by multivariate linear regression analysis. Conclusion there is a correlation between mt DNA copy number, mt DNA integrity and semen quality parameters., mt DNA may be a biomarker to reflect sperm quality.
【作者单位】：宁夏医科大学公共卫生与管理学院;第三军医大学军事预防医学院毒理学研究所;
【基金】：国家自然科学基金青年科学基金(81202276)~~
【分类号】：R698.2

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