优化处理技术对男性不育症患者精子形态和DNA碎片指数的影响

发布时间：2019-03-11 08:48

【摘要】：目的:确定优化处理技术对畸形精子症和不明原因不育症患者的精子形态和DNA碎片指数(DFI)的影响。方法:筛选2013年1月至2015年12月在我院就诊的400例男性及孕产妇家属,根据生育史和精液分析结果,分为正常生育组、畸形精子症组和不明原因不育症组,对其精液进行直接上游法和非连续密度梯度法优化处理,并分析其精子形态和DFI。结果:优化处理前畸形精子症组和不明原因不育症组精子头部、颈部畸形率和DFI均显著高于正常生育组(P0.01);优化处理后正常生育组、畸形精子症组和不明原因不育症组的精子畸形率和DFI均显著低于处理前(P0.01),经非连续密度梯度法处理的精子畸形率和DFI最低(P0.01)。结论:相对于直接上游法,非连续密度梯度法更有利于筛选形态正常和DNA完整性高的精子。
[Abstract]:Aim: to determine the effect of optimal treatment on sperm morphology and DNA fragment index (DFI) in patients with abnormal spermatozoospermia and infertility of unknown cause. Methods: from January 2013 to December 2015, 400 male and pregnant women's family members were selected and divided into normal fertility group, abnormal sperm group and unexplained infertility group according to the results of fertility history and semen analysis. The semen was optimized by direct upstream method and discontinuous density gradient method, and the sperm morphology and DFI. were analyzed. Results: the rate of abnormal sperm head, neck deformity and DFI were significantly higher in the abnormal spermatozoa group and the infertility group than those in the normal fertility group before the optimal treatment (P0.01). The sperm deformity rate and DFI in normal fertility group, abnormal sperm group and unexplained infertility group after optimized treatment were significantly lower than those before treatment (P0.01), and the sperm deformity rate and DFI in discontinuous density gradient method were the lowest (P0.01). Conclusion: compared with direct upstream method, discontinuous density gradient method is more favorable for screening sperm with normal morphology and high integrity of DNA.
【作者单位】：广东省妇幼保健院生殖健康与不孕症科;
【基金】：广东省中医药局科研课题资助项目(编号:20133001)
【分类号】：R698.2

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