RNA恒温扩增法与培养法检测解脲脲原体结果比较分析

发布时间：2019-04-17 06:40

【摘要】：目的:探讨实时荧光RNA恒温扩增检测(SAT)在男性不育患者精液标本中检测解脲脲原体(UU)的效果和临床意义。方法:收集2015年3~9月精液标本共662例,其中不育患者542例(不育组),已生育生殖健康检查人群标本120例(生育对照组),分别用培养法和SAT法同时检测以上标本。结果:培养法阳性的标本(除4例假阳性标本除外)SAT法检测全为阳性,其中不育组培养法与SAT法UU检出率分别为19.7%与54.1%(P0.05),生育对照组为12.5%与42.5%(P0.05)。用SAT法检测不育组和生育对照组其阳性率分别为51.4%和42.5%(P0.05),而用培养法检测不育组和生育对照组的阳性率分别为19.7%和12.5%(P0.05)。SAT法和培养法的检出率分别为52.0%和18.4%(P0.05),两种检测方法的总符合率为53.2%。结论:SAT技术可快速、准确检测UU的RNA,是未来发展方向,而培养法设备要求低,操作简单,可做药敏试验,应根据临床需要,灵活选用,提高UU的诊治水平。
[Abstract]:Aim: to investigate the effect and clinical significance of real-time fluorescence RNA constant temperature amplification for detection of Ureaplasma Urealyticum (UU) in semen samples of infertile men. Methods: a total of 662 semen samples were collected from March to September in 2015, including 542 infertile patients (infertility group) and 120 reproductive health check group (fertility control group). The above samples were detected by culture method and SAT method respectively. Results: all the positive samples (except 4 false positive samples) were positive by SAT method, and the positive rate of UU by culture method and SAT method were 19.7% and 54.1% (P0.05) in infertile group, respectively. The fertility control group was 12.5% and 42.5% (P0.05). The positive rate was 51.4% in infertile group and 42.5% in fertility control group by SAT method (P0.05). The positive rates were 19.7% and 12.5% in infertile group and fertility control group by culture method (P0.05) (52.0% and 18.4% by). SAT method and culture method respectively, P0.05), and the positive rate by culture method was 19.7% and 12.5% respectively (P0.05). The total coincidence rate of the two methods was 53.2%. Conclusion: the rapid and accurate detection of RNA, of UU by SAT technique is the developing direction in the future, but the culture method has the advantages of low equipment requirement, simple operation and drug sensitivity test. It should be selected flexibly according to the clinical needs to improve the diagnosis and treatment level of UU.
【作者单位】：成都西囡妇科医院检验科;宝鸡市人民医院泌尿外科;南方医科大学附属金陵医院/南京军区南京总医院男科;南方医科大学附属金陵医院/南京军区南京总医院医务部;南方医科大学附属金陵医院/南京军区南京总医院泌尿外科;南方医科大学附属金陵医院/南京军区南京总医院门诊部;
【基金】：江苏省社会发展面上项目(BE2017724)~~
【分类号】：R698.2

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