MicroRNA-4286在前列腺癌诊断中的价值

发布时间：2019-05-06 05:45

【摘要】：目的检测血清前列腺特异性抗原(prostate-specific antigen,PSA)升高患者microRNA-4286(miR-4286)的表达情况,探讨其在前列腺癌诊断中的意义。方法选择2016年3月至8月第二军医大学长海医院日间手术病区收治的171例血清PSA升高、行前列腺穿刺活检的患者,采用q PCR技术检测miR-4286在血清中的表达,比较前列腺癌(50例)和良性前列腺病变(121例)患者miR-4286表达的差异。并且分析10例良性病变患者术前与术后3 d的血清miR-4286的表达变化,以及15例PCa患者的术前与术后3 d及8例术前与术后7 d的血清miR-4286的表达变化。以受试者工作特征曲线分析评价miR-4286对PCa的诊断效能。结果 MiR-4286在前列腺癌和良性前列腺病变患者中的表达水平差异有统计学意义(P0.05)。术后3 d前列腺癌患者miR-4286的表达水平升高,与术前相比差异有统计学意义(P0.05),术后3 d良性前列腺病变患者miR-4286的表达水平与术前相比差异无统计学意义(P0.05);术后7 d miR-4286在前列腺癌患者中的表达水平与术前相比差异无统计学意义(P0.05)。MiR-4286和PSA在前列腺癌中的诊断效能分别为曲线下面积(AUC)0.653(界值3.72,敏感性68.75%,特异性60.06%)和0.733(界值11.38 ng/m L,敏感性57.16%,特异性92.35%)。结论 MiR-4286在前列腺癌中具有良好的诊断价值,可作为新型的液体活检标志物。
[Abstract]:Objective to detect the expression of microRNA-4286 (miR-4286) in patients with elevated serum prostate specific antigen (prostate-specific antigen,PSA) and to explore its significance in the diagnosis of prostate cancer. Methods A total of 171 patients with elevated serum PSA were selected from March to August 2016 in Changhai Hospital, second military Medical University. The expression of miR-4286 in serum was detected by Q-PCR technique. The difference of miR-4286 expression between prostate cancer (50 cases) and benign prostatic lesions (121 cases) was compared. We also analyzed the changes of serum miR-4286 expression in 10 patients with benign lesions before and 3 days after operation, and the changes of serum miR-4286 expression in 15 patients with PCa before and 3 days after operation and 8 patients before and 7 days after operation. The diagnostic efficacy of miR-4286 for PCa was evaluated by receiver operating characteristic curve analysis. Results there was a significant difference in the expression of MiR-4286 between prostate cancer and benign prostate disease (P0.05). On the 3rd day after operation, the expression of miR-4286 in patients with prostate cancer was significantly higher than that before operation (P0.05). There was no significant difference in the expression of miR-4286 between the patients with benign prostate disease and those before operation on the 3rd day after operation (P0.05). There was no significant difference in the expression of miR-4286 in patients with prostate cancer on the 7th day after operation (P0.05). The diagnostic efficacy of MiR-4286 and PSA in prostate cancer was (AUC) 0.653 (cutoff value 3.72, P < 0.05). The sensitivity was 68.75% and the specificity was 60.06%) and 0.733 (cutoff value 11.38 ng/m / L, sensitivity 57.16%, specificity 92.35%). Conclusion MiR-4286 has a good diagnostic value in prostate cancer and can be used as a new liquid biopsy marker.
【作者单位】：第二军医大学长海医院泌尿外科;
【基金】：国家自然科学基金重点项目(81430058) 上海市卫生和计划生育委员会联合攻关项目(2013ZYJB0101)~~
【分类号】：R737.25

【相似文献】