青蒿素联合羟基氯喹治疗IgA肾病大鼠的实验研究
发布时间：2019-06-10 23:12
【摘要】：目的探讨青蒿素联合羟基氯喹对IgA肾病大鼠的药理作用。方法采用联合牛血清白蛋白(bovine serum album,BSA)+脂多糖(Lipopolysaccharides,LPS)+四氯化碳(carbon tetrachloride,CCl4)方法建立Ig A肾病大鼠模型,选取尿总蛋白较高大鼠共50只,随机分成5组,即模型对照组、青蒿素与羟基氯喹1∶1组(16.7±16.7)mg·kg~(-1)·d~(-1)、青蒿素联合羟基氯喹1∶3组(8.3±25)mg·kg~(-1)·d~(-1)、血尿胶囊组(0.625 g·kg~(-1)·d~(-1))、地塞米松片组(0.078 mg·kg~(-1)·d~(-1)),每组10只,灌胃给药90 d。另取10只大鼠,不做任何处理,正常饲养作为正常对照组。给药90 d后,观察大鼠的一般情况,检测尿蛋白、血尿、血液生化、肾组织IgA荧光及电镜情况。结果与正常对照组比较,模型对照组尿蛋白、血尿及血清肌酐(GRE)、总蛋白(TP)、甘油三酯(TG)均显著增高(P0.05,P0.01),而血清尿素(UREA)明显降低(P0.01),血清IgA水平显著上升(P0.01),肾组织IgA荧光强度(++)~(+++),电镜检测肾小球发生明显病变。与模型对照组比较,青蒿素联合羟基氯喹1∶3组蛋白尿、血清IgA水平显著降低(P0.01),肾组织IgA荧光强度(+)显著降低,肾小球病变明显改善。结论运用BSA+LPS+CCl4方法造模15周可建立IgA肾病动物模型。青蒿素联合羟基氯喹可改善IgA肾病大鼠肾小球滤过膜的屏障功能,可调节Ig A肾病大鼠机体免疫反应,降低血清IgA水平,减少免疫复合物IgA沉积,改善IgA肾病大鼠肾脏病理损伤。
[Abstract]:Objective to investigate the pharmacological effects of artemisinin combined with hydroxychloroquine on IgA nephropathy rats. Methods the rat model of Ig A nephropathy was established by combining bovine serum albumin (bovine serum album,BSA lipopolysaccharide (Lipopolysaccharides,LPS) carbon tetrachloride (carbon tetrachloride,CCl4). A total of 50 rats with high urinary total protein were randomly divided into 5 groups. That is, the model control group, artemisinin and hydroxychloroquine 1:1 group (16.7 卤16.7) mg kg~ (- 1) d ~ (- 1), artemisinin combined with hydroxychloroquine group (8.3 卤25) mg kg~ (- 1) d ~ (- 1), In the hematuria capsule group (0.625 g kg~ (- 1) d ~ (- 1), dexamethasone tablets group (0.078 mg kg~ (- 1) d ~ (- 1), 10 rats in each group were given intragastrically for 90 days. Another 10 rats were taken without any treatment and fed normally as the normal control group. After 90 days of administration, the general situation of rats was observed, urine protein, hematuria, blood biochemistry, IgA fluorescence and electron microscope in renal tissue were detected. Results compared with the normal control group, the levels of urinary protein, hematuria and serum creatine (GRE), total protein (TP), (TG) in the model control group were significantly higher than those in the normal control group (P0.05, P01). However, serum urea (UREA) decreased significantly (P01), serum IgA level increased significantly (P01). Renal IgA fluorescence intensity () ~ (), electron microscope detected obvious pathological changes in glomeruli. Compared with the model control group, the serum IgA level in artemisinin combined with hydroxychloroquine 1:3 group was significantly lower than that in the model control group (P 0.01), the fluorescence intensity of IgA in renal tissue was significantly decreased, and the Glomerulopathy was significantly improved in artemisinin combined with hydroxychloroquine 1:3 group. Conclusion the animal model of IgA nephropathy can be established by BSA LPS CCl4 method for 15 weeks. Artemisinin combined with hydroxychloroquine can improve the barrier function of renal filtration membrane in rats with IgA nephropathy, regulate the immune response, decrease the level of serum IgA and decrease the deposition of immune complex IgA in rats with IgA nephropathy. To improve the pathological injury of kidney in rats with IgA nephropathy.
【作者单位】： 广州中医药大学;广州中医药大学科技产业园;广东新南方青蒿科技有限公司;
【基金】：广东省科技计划项目(2013B090800013,2013B090800024,2014B040404066,2014YT02S008)
【分类号】：R692.31
，
本文编号：2496803
[Abstract]:Objective to investigate the pharmacological effects of artemisinin combined with hydroxychloroquine on IgA nephropathy rats. Methods the rat model of Ig A nephropathy was established by combining bovine serum albumin (bovine serum album,BSA lipopolysaccharide (Lipopolysaccharides,LPS) carbon tetrachloride (carbon tetrachloride,CCl4). A total of 50 rats with high urinary total protein were randomly divided into 5 groups. That is, the model control group, artemisinin and hydroxychloroquine 1:1 group (16.7 卤16.7) mg kg~ (- 1) d ~ (- 1), artemisinin combined with hydroxychloroquine group (8.3 卤25) mg kg~ (- 1) d ~ (- 1), In the hematuria capsule group (0.625 g kg~ (- 1) d ~ (- 1), dexamethasone tablets group (0.078 mg kg~ (- 1) d ~ (- 1), 10 rats in each group were given intragastrically for 90 days. Another 10 rats were taken without any treatment and fed normally as the normal control group. After 90 days of administration, the general situation of rats was observed, urine protein, hematuria, blood biochemistry, IgA fluorescence and electron microscope in renal tissue were detected. Results compared with the normal control group, the levels of urinary protein, hematuria and serum creatine (GRE), total protein (TP), (TG) in the model control group were significantly higher than those in the normal control group (P0.05, P01). However, serum urea (UREA) decreased significantly (P01), serum IgA level increased significantly (P01). Renal IgA fluorescence intensity () ~ (), electron microscope detected obvious pathological changes in glomeruli. Compared with the model control group, the serum IgA level in artemisinin combined with hydroxychloroquine 1:3 group was significantly lower than that in the model control group (P 0.01), the fluorescence intensity of IgA in renal tissue was significantly decreased, and the Glomerulopathy was significantly improved in artemisinin combined with hydroxychloroquine 1:3 group. Conclusion the animal model of IgA nephropathy can be established by BSA LPS CCl4 method for 15 weeks. Artemisinin combined with hydroxychloroquine can improve the barrier function of renal filtration membrane in rats with IgA nephropathy, regulate the immune response, decrease the level of serum IgA and decrease the deposition of immune complex IgA in rats with IgA nephropathy. To improve the pathological injury of kidney in rats with IgA nephropathy.
【作者单位】： 广州中医药大学;广州中医药大学科技产业园;广东新南方青蒿科技有限公司;
【基金】：广东省科技计划项目(2013B090800013,2013B090800024,2014B040404066,2014YT02S008)
【分类号】：R692.31
，
本文编号：2496803
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