小鼠颈部迷走神经放电采集方法在肾缺血再灌注实验中的运用

发布时间：2019-06-17 08:40

【摘要】：目的观察研究C57BL/6J小鼠肾缺血再灌注期间颈部迷走神经放电变化,并介绍其迷走神经放电采集方法与技巧。方法雄性C57BL/6J小鼠8只,8~10周龄,体质量22~26 g,沿颈正中线切开皮肤,逐层分离至颈迷走神经,用铂金电极头引导神经放电并记录其放电频率;继而进行腹部手术,通过夹闭双侧肾门缺血25 min后再恢复血流30 min,该过程持续记录其迷走神经放电频率变化。结果与基础放电频率相比,肾缺血期间迷走神经放电频率由(520±52)Hz上升至(632±59)Hz(n=8,P0.05),再灌注后15 min内放电频率进一步升高至(672±58)Hz(n=8,P0.05),之后逐步下降至(544±51)Hz(n=8,P0.05)。结论肾缺血再灌注期间小鼠颈部迷走神经放电频率较基础放电频率明显上升,其原因可能涉及缺血再灌注时自身的神经保护性机制。
[Abstract]:Objective to observe and study the changes of cervical vagus nerve discharge during renal ischemia-reperfusion in C57BL/6J mice, and to introduce the methods and techniques of vagus nerve discharge collection. Methods eight male C57BL/6J mice, 8 脳 10 weeks old and weighing 22 脳 26 g, were cut off along the median cervical line and separated into cervical vagus nerve layer by layer. The discharge frequency of vagus nerve was guided by platinum electrode head and recorded, and then abdominal surgery was performed to record the changes of vagus nerve discharge frequency by clamping bilateral hilar ischemia for 25 min and then resuming blood flow for 30 min,. Results compared with the basic discharge frequency, the discharge frequency of vagus nerve increased from (520 卤52) Hz to (632 卤59) Hz (n 鈮，

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