PKCzeta在前列腺癌组织中的表达及其对前列腺癌细胞增殖的影响
发布时间：2019-07-27 07:55
【摘要】：目的检测PKCzeta在不同分级前列腺癌(PCa)组织的表达情况,并探究PKCzeta的表达对前列腺癌细胞系增殖的影响。方法采用免疫组织化学染色法检测19例前列腺癌组织和4例正常前列腺组织的PKCzeta表达情况,并按肿瘤分级分为3组,进行PKCzeta表达的差异性分析;应用Hilymax试剂转染PKCzeta质粒(CA-PKCzeta)过表达前列腺癌细胞PKCzeta;通过实时荧光定量PCR(RT-qPCR)和Western blot分别检测PC3和DU145细胞内PKCzeta的mRNA和蛋白水平;利用平板克隆形成试验和CCK-8试剂盒检测PC3和DU145细胞增殖能力的差异以及转染PKCzeta质粒后细胞增殖的变化。结果 PKCzeta在前列腺癌组织中的表达高于正常前列腺组织(P0.05),而且低分级(1级)前列腺癌PKCzeta的表达高于高分级(2~3级)前列腺癌(P0.05)。DU145细胞的PKCzeta表达高于PC3细胞,而其增殖速度较PC3细胞低;过表达PKCzeta使前列腺癌细胞系的增殖受抑。结论 PKCzeta与肿瘤分级存在负相关关系,在癌症早期可能有一定的保护作用,且过表达PKCzeta能抑制前列腺癌细胞的增殖。
[Abstract]:Objective to detect the expression of PKCzeta in different grades of prostate cancer (PCa) and to explore the effect of PKCzeta expression on the proliferation of prostate cancer cell line. Methods Immunohistochemical staining was used to detect the expression of PKCzeta in 19 cases of prostate cancer and 4 cases of normal prostate tissue, and the expression of PKCzeta was divided into three groups according to tumor grade, and the mRNA and protein levels of PKCzeta in PC3 and DU145 cells were detected by real-time fluorescence quantitative PCR (RT-qPCR) and Western blot respectively. Plate clone formation test and CCK-8 kit were used to detect the difference of proliferation ability between PC3 and DU145 cells and the change of cell proliferation after PKCzeta plasmid transfer. Results the expression of PKCzeta in prostate cancer was higher than that in normal prostate (P 0.05), and the expression of PKCzeta in low grade (grade 1) prostate cancer was higher than that in high grade (2 鈮，
本文编号：2519865
[Abstract]:Objective to detect the expression of PKCzeta in different grades of prostate cancer (PCa) and to explore the effect of PKCzeta expression on the proliferation of prostate cancer cell line. Methods Immunohistochemical staining was used to detect the expression of PKCzeta in 19 cases of prostate cancer and 4 cases of normal prostate tissue, and the expression of PKCzeta was divided into three groups according to tumor grade, and the mRNA and protein levels of PKCzeta in PC3 and DU145 cells were detected by real-time fluorescence quantitative PCR (RT-qPCR) and Western blot respectively. Plate clone formation test and CCK-8 kit were used to detect the difference of proliferation ability between PC3 and DU145 cells and the change of cell proliferation after PKCzeta plasmid transfer. Results the expression of PKCzeta in prostate cancer was higher than that in normal prostate (P 0.05), and the expression of PKCzeta in low grade (grade 1) prostate cancer was higher than that in high grade (2 鈮，
本文编号：2519865
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