1-溴丙烷对雄性大鼠血浆和脑组织中NSE、MBP蛋白表达影响

发布时间：2017-12-28 14:21

本文关键词：1-溴丙烷对雄性大鼠血浆和脑组织中NSE、MBP蛋白表达影响　出处：《中国职业医学》2016年03期 　论文类型：期刊论文

【摘要】：目的探讨1-溴丙烷(1-BP)亚急性吸入染毒对雄性大鼠血浆和脑组织中神经元特异性烯醇化酶(NSE)和髓鞘碱性蛋白(MBP)的影响。方法无特定病原体级成年雄性Wistar大鼠随机分为对照组和低、中、高剂量组,每组12只,分别予质量浓度为0、1 250、2 500、5 000 mg/m3的1-BP连续动式吸入染毒,每天染毒6 h,每周5 d,连续4周。染毒结束后,各组随机取9只大鼠,经腹主动脉采血,分离血浆,采用免疫酶联吸附法检测其NSE和MBP水平,分离全脑、大脑、小脑和脑干检测脏器系数;各组另3只大鼠行脑病理组织学检查,并采用免疫组织化学法检测脑组织中NSE和MBP的蛋白表达。结果高剂量组大鼠全脑、大脑、小脑及脑干的脏器系数分别高于对照组[(0.754±0.056)%vs(0.663±0.035)%,(0.382±0.037)%vs(0.339±0.021)%,(0.115±0.008)%vs(0.098±0.006)%,(0.213±0.018)%vs(0.183±0.014)%,P0.01]。低、中和高剂量组大鼠血浆中NSE水平分别低于对照组[(7.92±0.53)vs(24.73±11.44)mg/L,(9.12±2.17)vs(24.73±11.44)mg/L,(11.10±2.84)vs(24.73±11.44)mg/L,P0.01];4组大鼠血浆中MBP水平比较,差异无统计学意义[(2.52±0.70)vs(2.50±0.72)vs(2.47±0.66)vs(2.44±0.81)mg/L,P0.05]。脑组织病理学检查结果显示:仅在高剂量组大鼠大脑海马区观察到少量坏死的神经元。低、中剂量组和对照组大鼠脑组织中NSE、MBP的蛋白表达量无明显差异,仅在高剂量组大鼠大脑海马区观察到NSE和MBP蛋白表达量下调的细胞。结论 1-BP所致的神经毒性对中枢神经功能影响比结构影响更为明显;血浆中NSE可能是1-BP暴露的效应标志物之一。
[Abstract]:Objective to investigate the effects of subacute inhalation of 1- bromo propane (1-BP) on neuron specific enolase (NSE) and myelin basic protein (MBP) in plasma and brain of male rats. Methods adult male Wistar rats without specific pathogen level were randomly divided into control group and low, medium and high dose group, 12 rats in each group. They were given continuous inhalation of 1-BP at 0, 1250, 2500 and 5000 mg/m3, respectively, and they were exposed to 6 h daily, 5 d per week for 4 weeks. After the end of the exposure group, 9 rats were randomly selected, the abdominal aortic blood plasma separation, by using enzyme linked immunosorbent assay to detect the NSE and MBP levels, the separation of the whole brain, brain, cerebellum and brainstem detect organ coefficient; another group of 3 rats for brain histopathology, and the expression of NSE and MBP in mining immunohistochemical method was used to detect the protein in brain tissue. Results the organ coefficients of the whole brain, brain, cerebellum and brainstem in the high-dose group were higher than those in the control group [(0.754 + 0.056)%vs (0.663 + 0.035)%, (0.382 + 0.037)%vs (0.339 + 0.021)%, (0.115 + 0.008)%vs (0.098%, 0.098%), (0.098 +%vs) (P0.01] +%), P0.01]. Low, medium and high dose group in rat plasma NSE levels were lower than the control group [(7.92 + 0.53) vs (24.73 + 11.44) mg/L, (9.12 + 2.17) vs (24.73 + 11.44) mg/L, (11.10 + 2.84) vs (24.73 + 11.44) mg/L, P0.01]; compare the level of MBP in 4 groups in plasma, there was no significant difference between [(2.52 + 0.70) vs (2.50 + 0.72) vs (2.47 + 0.66) vs (2.44 + 0.81) mg/L, P0.05]. The results of brain histopathological examination showed that only a small amount of necrotic neurons were observed in the hippocampus of the high dose rats. There was no significant difference in the expression of NSE and MBP between the low and middle dose group and the control group. Only in the hippocampus of high dose group, the expression of NSE and MBP protein was down regulated. Conclusion the effect of 1-BP induced neurotoxicity on central nervous function is more obvious than that of the structure, and NSE in plasma may be one of the markers of 1-BP exposure.
【作者单位】：广东省职业病防治院广东省职业病防治重点实验室;山西医科大学公共卫生学院;
【基金】：国家自然科学基金(81202246) 广东省职业病防治重点实验室(2012A061400007) 广东省科技计划项目(2014A020212500) 广东省医学科研基金(A2015344)
【分类号】：R135.1
【正文快照】： 近年来,1-溴丙烷(1-Bromopropane,1-BP)在工业上常被作为含氟化合物的替代品,用于脱脂、干洗、喷胶和气溶胶溶剂等[1-2],其对人类健康危害及其防治的研究成为热点。2013年我国修订施行的《职业病分类和目录》中,溴丙烷中毒作为新增的18种职业病之一被纳入职业性化学中毒类职业

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