酵母源金属硫蛋白对慢性汞中毒小鼠氧化损伤的修复作用

发布时间：2017-12-30 16:05

本文关键词：酵母源金属硫蛋白对慢性汞中毒小鼠氧化损伤的修复作用　出处：《食品科学》2016年15期 　论文类型：期刊论文

【摘要】：探讨具有自主知识产权的两种酵母源金属硫蛋白(metallothionein,MT)亚型(MT-1和MT-2)对慢性汞中毒小鼠氧化损伤的修复作用。实验小鼠经氯化汞染毒,连续灌胃两种酵母源MT及二巯基丙磺酸钠(dimercaptopropansulfonate sodium,DMPS)28 d后测定小鼠体质量、全血汞含量及血清超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活力,丙二醛(malondialdehyde,MDA)含量及机体总抗氧化能力(total antioxidative capacity,T-AOC)。与正常对照组小鼠相比,模型对照组小鼠体质量显著下降(P0.05),灌胃给药28 d后,两种酵母源MT亚型高剂量组小鼠体质量显著升高(P0.05);各给药组小鼠血液、肝脏及肾脏汞含量显著低于模型对照组(P0.05),两种酵母源MT亚型高剂量组的排汞效果显著优于DMPS组(P0.05);各给药组小鼠血清中GSH-Px、SOD活力和MDA水平及总抗氧化能力接近正常对照组小鼠水平(P0.05),其中酵母源MT高剂量组的作用效果最佳,且在改善机体T-AOC方面的效果优于DMPS组(P0.05),酵母源MT-1亚型升高小鼠血清SOD活力和降低血清MDA含量的作用效果明显好于酵母源MT-2亚型。两种酵母源MT对慢性汞中毒小鼠具有良好的氧化损伤保护作用及排汞效果。
[Abstract]:To investigate the source of two yeast metallothionein with independent intellectual property rights (metallothionein, MT) subtypes (MT-1 and MT-2) in repair of oxidative damage in mice of chronic mercury. The experimental mice were treated with mercuric chloride, continuous intragastric administration of two yeast derived MT and DMPS (dimercaptopropansulfonate sodium, DMPS) 28 d after the determination of body weight in mice, the mercury content of serum and blood superoxide dismutase (superoxide dismutase SOD), glutathione peroxidase (glutathione peroxidase, GSH-Px) activity, malondialdehyde (malondialdehyde, MDA) content and total antioxidant capacity (total antioxidative body capacity, T-AOC). Compared with mice in normal control group, model control group body weight of mice was significantly decreased (P0.05), intragastric administration of 28 d, two kinds of yeast derived MT subtype high dose group of mice was significantly increased (P0.05); each group of mice blood and liver. The mercury content in the kidney was significantly lower than the model control group (P0.05), two yeast derived MT subtype high dose group Hg effect was better than that of group DMPS (P0.05); the serum GSH-Px, SOD activity and MDA level and total antioxidant capacity close to mice in normal control group (P0.05), the effect of the level of the effect of yeast source MT high dose group is best, and in better than group DMPS (P0.05) T-AOC of the body, the effect of yeast derived MT-1 subtype increased serum SOD activity in mice and reduce serum MDA content was significantly better than that of yeast derived MT-2 subtype. Two kinds of yeast source MT has good protective effect on oxidation damage and mercury emission effect on chronic mercury poisoning in mice.

【作者单位】：黑龙江八一农垦大学食品学院;国家杂粮工程技术研究中心;青岛琅琊台集团股份有限公司;
【基金】：黑龙江省博士后资助项目(LBH-Z13168) 黑龙江省自然科学基金项目(C201445) 黑龙江省农垦总局“十二五”重点科技计划项目(HNK125B-13-05) 黑龙江省新世纪优秀人才培养计划项目(2014-2016)
【分类号】：R595.2
【正文快照】： 汞作为一种毒性IIB族金属,广泛存在于自然和人类1.2材料与试剂生活环境之中。汞通常可制成消毒剂、止痛剂和治疗疥酵母源金属硫蛋白亚型(MT-1、MT-2,纯度疮的药膏,并可添加于化妆品祛斑霜中,用以对脸部遮95%),黑龙江八一农垦大学食品学院实验室分离纯化瑕及达到祛斑的效果。同

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