糖尿病模型大鼠海马区星形胶质细胞活化与凋亡的观察

发布时间：2018-01-19 04:37

本文关键词： 星形胶质细胞活化细胞凋亡高血糖胶质原纤维酸性蛋白海马大鼠　出处：《神经解剖学杂志》2016年01期 　论文类型：期刊论文

【摘要】：目的:探讨糖尿病高血糖状态对于大鼠海马星形胶质细胞的影响。方法:应用链脲佐菌素(STZ)诱导Ⅰ型糖尿病SD大鼠模型,分为糖尿病模型1、2、3、4周和5周组(DM1,DM2,DM3,DM4,DM5),同周龄SD大鼠作为对照组。采用免疫荧光、免疫组化和Western Blot等技术,对比观察糖尿病大鼠海马区星形胶质细胞的形态、caspase-3和GFAP的表达情况。结果:免疫荧光和免疫组化检测结果显示:DM1和DM2大鼠海马区星形胶质细胞的胞体增大,突起增粗;DM3和DM4大鼠海马内星形胶质细胞的突起增粗、变长,其数量明显多于正常对照组(P0.05);而DM5大鼠海马内星形胶质细胞的突起僵硬,细胞数量有所减少,但仍高于正常对照组(P0.05)。Western Blot结果显示:DM3,DM4,DM5大鼠海马区GFAP含量明显高于对照组和DM1,DM2(P0.05)。caspase-3/GFAP免疫组化双标结果显示:DM1和DM2大鼠海马区偶见caspase-3阳性标记的星形胶质细胞;而DM3,DM4,DM5大鼠海马区caspase-3/GFAP双标细胞数明显多于正常对照组(P0.05);其中DM5双标阳性细胞数明显多于DM3和DM4(P0.05)。结论:糖尿病高血糖早期可激活星形胶质细胞,持续性糖尿病高血糖可诱导海马区星形胶质细胞活化的抑制,并引起星形胶质细胞凋亡。
[Abstract]:Objective: to investigate the effect of diabetic hyperglycemia on hippocampal astrocytes in rats. Methods: SD rats with type I diabetes were induced by streptozotocin (STZ) and divided into two groups. The SD rats of the same age were used as the control group. Immunohistochemical and Western Blot techniques were used to observe the morphology of astrocytes in the hippocampus of diabetic rats. Results: the expression of caspase-3 and GFAP in hippocampal astrocytes of rats with caspase-3 and DM2 were increased and the processes were thickened. The number of astrocytes in hippocampus of DM3 and DM4 rats was significantly higher than that of normal control group (P 0.05). The astrocytes in hippocampus of DM5 rats were stiff, and the number of astrocytes was decreased, but still higher than that of normal control group (P 0.05). Western Blot showed that: DM3. The content of GFAP in hippocampus of DM4 / DM5 rats was significantly higher than that of control group and DM1. DM2 (P0.05). The double labeling results of caspase-3 / GFAP immunohistochemistry showed that caspase-3 positive astrocytes were occasionally found in hippocampus of DM2 rats. The number of caspase-3/GFAP double labeled cells in the hippocampal area of DM3 + DM4 + DM5 rats was significantly higher than that of the normal control group (P0. 05). The number of DM5 double labeled positive cells was significantly higher than that of DM3 and DM4 P0.050.Conclusion: early diabetic hyperglycemia can activate astrocytes. Hyperglycemia can induce the inhibition of astrocyte activation and astrocyte apoptosis in hippocampus.
【作者单位】：宁夏医科大学基础医学院宁夏颅脑疾病重点实验室-省部共建国家重点实验室培育基地;德国国家分子医学研究中心;
【基金】：国家自然科学基金(81360184) 教育部“春晖计划”项目(Z2011058) 宁夏医科大学优势学科群建设科研项目(2014023)
【分类号】：R587.1;R-332
【正文快照】： 糖尿病模型大鼠海马区星形胶质细胞活化与凋亡的观察张娜1△,孟令超1△,张竞文1,常越1,景丽1,安俊峰2,张建忠1*(1.宁夏医科大学基础医学院,宁夏颅脑疾病重点实验室-省部共建国家重点实验室培育基地,银川750004;2.德国国家分子医学研究中心,柏林13125)糖尿病是一组以高血糖为特

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