sLR11基因多态性与T2DM及颈动脉粥样硬化的相关性研究

发布时间：2018-01-20 08:14

本文关键词： sLR11基因多态性 2型糖尿病颈动脉IMT　出处：《延边大学》2016年硕士论文　论文类型：学位论文

【摘要】：研究目的：探讨延边地区朝鲜族与汉族2型糖尿病患者可溶性低密度脂蛋白受体11(Soluble loW- ensity lipoprotein recptor fami— ly member11, sLR11).单核苷酸多态性与动脉硬化之间的相关性。研究对象：选择2012年12月至2015年12月在延边大学附属医院内分泌门诊及住院治疗2型糖尿病患者350例,根据所选研究对象及颈动脉IMT值分为颈动脉粥样硬化组(CAL组,n=163例,2型糖尿病颈动脉IMT=1.0mm伴斑块组)和无颈动脉动脉粥样硬化组(NCAL组,n=187例,2型糖尿病颈动脉IMT1.0mm组)。正常对照组(NGT组,n=172例,健康人)。研究方法：通过彩色超声诊断仪测定颈动脉IMT及斑块。利用LDR-PCR技术进行SNP分型及测序。运用Hardy-Weinberg平衡定律,确定研究样本的群体代表性。多组间计量资料的比较采用one-way ANOVA,其中两两比较采用LSD法比较,应用Logistic回归分析筛选颈动脉粥样硬化的危险因素,以P0.05为有统计学意义。结果：1.延边地区CAL组、NCAL组、NGT组等位基因A、T频率分别为54.3%,45.7%; 56.7%,43.3%； 58.1%,41.9%。基因型AA.AT.TT频率分别为27.6%,53.4%,1 9.0%；32.1%,49.2%,18.7%；30.2%,55.8%,14.0%。经检验CAL组与NGT组、NCAL组与NGT组、CAL组与NCAL组基因型及等位基因频率分布均无统计学差异,P0.05。并进行显性、隐性模式分析,但未见各基因型频率在三组间比较存在显著差异,P0.05。2.在CAL组朝鲜族、汉族人群等位基因A、T基因频率分别为50.7%,49.3％:55.3％,44.7％；AA.AT.TT基因型频率分别为24.3%,52.7%,23.0%；27.1%,56.5%,16.5%。NCAL组朝鲜族、汉族人群等位基因A、T基因频率分别为53.3%,46.7%；58.5%,41.5%AA、AT、TT基因型频率分别为23.7%,59.2%,17.1%;36.8%,43.4%,19.8%。NGT组朝鲜族、汉族人群等位基因A、T基因频率分别53.8%,46.2%；60.5%,39.5%；AA、AT、TT基因型频率分别为26.2%,55.4%,18.5%；33.0%,55.0%,12.0%。经检验CAL组、NCAL组、NGT组中朝鲜族与汉族人群基因型频率分布均无统计学差异,(P0.05)。3. SBP, LDL-C, HbAlc的回归系数分别为-0.024,-0.585, -0.251,回归系数与颈动脉硬化成正相关,由此可知,SBP, LDL-C, HbAlc是颈动脉粥样硬化的危险因素。4.对2型糖尿病颈动脉硬化组中三组基因型进行组间年龄及各代谢指标进行统计学分析,CHO在AT基因型组中高于AA基因型组高于TT基因型组,AT组与AA组、AT组与TT组有统计学差异,P0.05。TG在AT基因型组中高于TT基因型组,两组之间有统计学差异,P0.01。LDL-C在TT基因型组中高于AT基因型组高于AA基因型组,两两比较均有统计学差异,P0.01。结论：1.sLR11基因rs3824968多态性与延边地区2型糖尿病无明显相关性。2.sLR11基因rs3824968多态性在延边地区朝鲜族与汉族人群中无种族差异。3.sLR11基因rs3824968多态T因子可能通过影响LDL的降解,从而增加T2DM罹患颈动脉粥样硬化的风险。4. SBP,LDL-C,HbAlc为颈动脉粥样硬化的危险性因素。
[Abstract]:Objective: to study the soluble low density lipoprotein receptor (sLDLR) 11 in Korean and Han patients with type 2 diabetes mellitus in Yanbian area. Soluble low-ensity lipoprotein recptor fami- ly member11. SLR11). Association of single nucleotide polymorphisms with atherosclerosis. Subjects:. From December 2012 to December 2015, 350 patients with type 2 diabetes mellitus were treated in the endocrine clinic and inpatient department of Yanbian University affiliated Hospital. According to the selected subjects and carotid IMT value, the patients were divided into carotid atherosclerosis group (n = 163) and carotid atherosclerosis group (n = 163). Type 2 diabetic carotid artery IMT=1.0mm with plaque group (n = 187) and no carotid atherosclerosis group (n = 187). Type 2 diabetes mellitus (IMT1.0mm) group and normal control group (n = 172). Healthy persons). Methods: carotid IMT and plaque were determined by color ultrasound. SNP typing and sequencing were performed by LDR-PCR. Hardy-Weinberg equilibrium law was used. One-way ANOVA was used to compare the multi-group measurement data, and the LSD method was used to compare the two groups. Logistic regression analysis was used to screen the risk factors of carotid atherosclerosis, with P0.05 as the statistical significance. Results: 1. Yanbian area CAL group NCAL group. In NGT group, the frequency of Agna T was 54.3% and 45.7%, respectively. 56.7 and 43.3; The frequency of genotype AA.AT.TT was 27.6 and 53.4%, respectively. 32.1 there are 49.2 and 18.7; 30.2NCAL and NGT groups were examined in CAL and NGT groups. There was no significant difference in genotype and allele frequency distribution between CAL group and NCAL group (P 0.05). The dominant and recessive patterns were analyzed. However, there was no significant difference in genotype frequencies among the three groups (P0.05.2). In CAL group, the allelic alleles of Agna T gene were 50.7% in Korean nationality and Han nationality, respectively. 49.3: 55.3and 44.7; The frequencies of AA.AT.TT genotypes were 24. 3% and 52. 7% and 23. 0%, respectively. The frequencies of allelic allele A T gene were 53.33,46.7 in the Korean nationality and the Han nationality in the 56.5and 16.5. NCAL groups, respectively. The frequencies of ATT TT genotypes were 23.7and 59.2and 17.1respectively. The frequencies of allelic allele A T gene in the NGT group were 53.8% and 46.2%, respectively. 60.5 and 39.5; The frequencies of ATT TT genotypes were 26.2and 55.4%, respectively. There was no significant difference in genotype frequency distribution between Korean and Han population in CAL group. The regression coefficients of SBP, LDL-C and HbAlc were -0.024 ~ 0.585 and -0.251 respectively. The regression coefficient was positively correlated with carotid atherosclerosis, which showed that SBP, LDL-C. HbAlc is the risk factor of carotid atherosclerosis. (4) the age and metabolic indexes of three genotypes in type 2 diabetes mellitus with carotid atherosclerosis were analyzed statistically. CHO was higher in AT genotype group than in AA genotype group than in TT genotype group. There was statistical difference between AT group and AA group and AT group and TT group. P0.05.TG in AT genotype group was higher than TT genotype group, there was statistical difference between two groups. P0.01. LDL-C was higher in TT genotype group than in AT genotype group than in AA genotype group. P0.01. conclusion:. 1. The rs3824968 polymorphism of sLR11 gene is not associated with type 2 diabetes mellitus in Yanbian area. 2.sLR11 gene rs3824968 polymorphism in Korean and Han nationality in Yanbian area. The rs3824968 polymorphism T factor of sLR11 gene may influence the degradation of LDL. Therefore, the risk of carotid atherosclerosis in T2DM was increased. 4. SBP LDL-Con HbAlc was the risk factor of carotid atherosclerosis.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R587.2

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