葛根素对过氧化氢致成骨细胞氧化损伤的影响

发布时间：2018-01-29 17:00

本文关键词： 葛根素成骨细胞过氧化氢　出处：《中国骨质疏松杂志》2017年11期 　论文类型：期刊论文

【摘要】：目的研究具有抗氧化作用的植物雌激素——葛根素(puerarin,Pue)对氧化应激后成骨细胞(osteoblast,OB)的影响。方法采用酶多次消化法,由出生24 h以内的Wistar大鼠头盖骨分离OB,培养、传代及诱导分化、矿化等。用0.1 mmol/L H_2O_2制造氧化应激状态模型。将细胞随机分为对照组、H_2O_2组[H'组]、"Pue(10~(-10)mol/L~10~(-3)mol/L)+H_2O_2"组["P+H'"组]和"H_2O_2+Pue(10~(-10)mol/L~10~(-3)mol/L)"组["H'+P"组]。观察不同处理组细胞的增殖、分化功能,并测定其微量丙二醛(m MDA)含量、超氧化物歧化酶(super oxide dismutase,SOD)活性、总抗氧化能力(T-AOC)等。结果与H_2O_2组相比,"P+H'"组和"H'+P"组中Pue10~(-7)mol/L~10~(-4)mol/L使细胞的增殖、分化功能,SOD活性和T-AOC均有显著升高(P0.01),而mMDA含量则显著降低(P0.01)。结论 H_2O_2过量会导致OB的氧化损伤,而葛根素在体外对H_2O_2介导OB的氧化损伤具有保护作用。
[Abstract]:Objective to study the effects of phytoestrogen-puerarin puerarin on osteoblast of osteoblasts after oxidative stress. Methods OB was isolated from the cranium of Wistar rats within 24 hours after birth, cultured, subcultured and induced to differentiate. Mineralization, etc. The oxidative stress state model was made by using 0.1 mmol/L Hs _ 2O _ 2. The cells were randomly divided into two groups: control group (H _ S _ 2O _ 2). [Group H', "Puehl 10 / 10 / L 10 / 10 / 3 mol / L) H _ 2O _ 2s _ 2" [Group "P H'"] and "H2O2\\\'\\\'s 10 / 10 / 10 / 10 / 10 / L\ + 10 / 10 / L\\\ {10\}\' [The proliferation and differentiation function of cells in different treatment groups were observed, and the content of malondialdehyde (MDA) m MDAs was measured. Superoxide dismutase superoxide dismutase (oxide dismutase) activity and total antioxidant capacity (T-AOC) were compared with those in group H _ 2O _ 2. Pue10~(-7)mol/L~10~(-4)mol/L in "P''" group and "H' P" group resulted in cell proliferation and differentiation. The activity of SOD and T-AOC increased significantly, while the content of mMDA decreased significantly. Conclusion the excessive amount of H _ 2O _ 2 can lead to oxidative damage of OB. Puerarin has protective effect on oxidative damage of OB induced by H _ 2 O _ 2 in vitro.
【作者单位】：西安交通大学医学院附属广仁医院/西安市第四医院;兰州大学第一医院内分泌科;兰州军区兰州总医院骨科研究所;西安市灞桥区中医医院;
【分类号】：R580
【正文快照】： 随着人口老龄化日趋明显和人们对生活质量要求的提高,骨质疏松及其并发症已成为全世界关注的一大社会保健和公共卫生问题,号称“世纪慢性隐形疾病”,受到各国医学界和国际社会的普遍重视[1]。尤其是妇女绝经后骨质疏松症(postmenopausal osteoporosis,PMOP)发病率高,危害性大,

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