EGR-1在硬皮病皮损中表达的临床意义

发布时间：2018-01-30 09:07

本文关键词： 硬皮病 EGR-1 皮肤纤维化免疫组织化学　出处：《广西医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：目的初步探讨EGR-1在硬皮病及其它几种常见纤维化皮肤病皮损中的表达及临床意义,初步阐明其在硬皮病中具有非疾病特异性的促纤维化作用。方法分别收集系统性硬皮病(SSc组)20例,局限性硬皮病(LSc组)14例,硬化性萎缩性苔藓(LSA组)10例,增生性瘢痕(HS组)6例,正常对照(NOR组)6例的皮损组织蜡块。采用免疫组织化学方法检测EGR-1在血管内皮细胞、成纤维细胞的表达情况。用SPSS 22.0统计软件进行统计学分析。结果各组组织切片EGR-1阳性细胞的平均光密度值分别是:SSc组0.006044±0.003809,LSc组0.002740±0.001540,LSA组0.009780±0.003874,HS组0.0251111±0.010022,NOR组0.000972±0.000194。组间比较:SSc、LSc、LSA及HS患者皮损内均表达EGR-1,除LSc组与NOR组比较表达量的差异无统计学意义(P0.05)外,其余两两之间比较差异均有统计学意义(均P0.05)。其表达量按高低排序为:HS组、LSA组、SSc组、LSc组、NOR组。结论EGR-1有促皮肤纤维化作用,但EGR-1可能不是硬皮病纤维化进程的特异性促进因子;EGR-1有望成为抗纤维化治疗的重要靶点。
[Abstract]:Objective to investigate the expression and clinical significance of EGR-1 in skin lesions of scleroderma and other common fibrosis skin diseases. Methods 20 patients with systemic scleroderma and 14 patients with LSC with localized scleroderma were collected. 10 cases in LSA group and 6 cases in hypertrophic scar HS group. EGR-1 in vascular endothelial cells was detected by immunohistochemical method in 6 cases of skin lesions in normal control nor group. The expression of fibroblasts was analyzed by SPSS 22.0 software. Results the average optical density of EGR-1 positive cells in tissue sections of each group were:. In SSc group, 0.006044 卤0.003809. LSc group 0.002740 卤0.001540LSA group 0.009780 卤0.003874HS group 0.0251111 卤0.010022. NOR group 0.000972 卤0.0001944.The expression of EGR-1 in the lesions of patients with NOR and HS was compared. There was no significant difference in expression between LSc group and NOR group (P 0.05). The differences between the other two groups were statistically significant (P 0.05). The order of the expression of the two groups was: 1 / HS group / LSA group / SSC group / LSc group. Conclusion EGR-1 can promote skin fibrosis, but EGR-1 may not be a specific promoter of scleroderma fibrosis. EGR-1 is expected to be an important target of anti-fibrosis therapy.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R593.25

【参考文献】