慢性高原病患者骨髓有核红细胞Ras-GTP分泌水平及BRaf、MEK、ERK1、ERK2 mRNA表达变化

发布时间：2018-02-02 12:45

本文关键词： 高原病骨髓有核红细胞 Ras/Raf/MEK/ERK信号通路　出处：《山东医药》2016年03期 　论文类型：期刊论文

【摘要】：目的观察慢性高原病(CMS)患者骨髓有核红细胞Ras相关GTP酶(Ras-GTP)分泌水平及B型丝/苏氨酸蛋白激酶(BRaf)、丝裂原活化蛋白激酶(MEK)、细胞信号调节激酶1(ERK1)、细胞信号调节激酶2(ERK2)表达变化。方法 CMS患者15例(观察组),单纯陈旧性骨折患者15例(对照组),采用ELISA法检测骨髓有核红细胞Ras-GTP,实时荧光定量PCR法检测骨髓有核红细胞BRaf、MEK、ERK1、ERK2 mRNA。结果与对照组比较,观察组Ras-GTP分泌水平升高,BRaf、MEK、ERK1、ERK2 mRNA的相对表达量增加(P均0.05)。观察组Ras-GTP、Braf mRNA、MEK mRNA、ERK1 mRNA、ERK2 mRNA与HGB呈正相关(r分别为0.860、0.849、0.653、0.773、0.746,P均0.01)。结论 CMS患者骨髓有核红细胞Ras-GTP分泌水平及BRaf、MEK、ERK1、ERK2表达升高,Ras/Raf/MEK/ERK通路可能与CMS红细胞的过度累积有关,推测其可能是CMS发病机制中的重要通路之一。
[Abstract]:Objective to observe the level of Ras-GTPsecretion of nucleated red blood cells (Ras) and B-type serine / threonine protein kinase (BRafA) in patients with chronic high altitude disease (CMS). The expression of mitogen activated protein kinase (MEKK), cell signal regulated kinase 1 (ERK1) and cell signal regulated kinase (2 ERK2) were observed in 15 patients with CMS (observation group). Ras-GTPof nucleated erythrocytes in bone marrow was detected by ELISA method and BRaf of nucleated erythrocytes in bone marrow was detected by real-time fluorescence quantitative PCR in 15 patients with simple old fracture (control group). Results compared with the control group, the level of Ras-GTP secretion in the observation group was higher than that in the control group. The relative expression of ERK2 mRNA was increased (P < 0.05). The Ras-GTPTP-Braf mRNA-MEK mRNA-ERK1 mRNA was observed in the observation group. The positive correlation between ERK2 mRNA and HGB was 0.860 ~ 0. 849 ~ 0. 653 ~ 0. 773 ~ 0. 746, respectively. Conclusion the level of Ras-GTP secretion and the expression of ERK1 ERK2 in bone marrow nucleated erythrocytes in patients with CMS were increased. The Ras/Raf/MEK/ERK pathway may be related to the excessive accumulation of CMS erythrocytes, which may be one of the important pathways in the pathogenesis of CMS.
【作者单位】：青海大学医学院;青海大学附属医院;青海大学;青海省人民医院;
【基金】：青海省科技厅国际合作项目(2014-HZ-808) 国家自然科学基金资助项目(81441116) 青海省科技厅(应用)基础研究计划项目(2012-Z-729)
【分类号】：R594.3
【正文快照】： 慢性高原病(CMS)既往又称为高原红细胞增多症,以过度红细胞增多和严重低氧血症为特征[1],并伴有血红蛋白(HGB)及红细胞压积升高,但目前其发病机制尚未完全阐明。研究[2]认为,CMS发生病理生理改变的主要机制是机体对高原环境失适应,导致细胞内低氧,引起低氧诱导因子表达增加,促

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