合并与不合并2型糖尿病的下肢动脉硬化闭塞症患者血清差异性蛋白组学分析

发布时间：2018-02-03 07:25

  本文关键词： 型糖尿病 下肢动脉硬化闭塞症 蛋白组学 同位素标记 二维液相色谱串联质谱 差异性蛋白　出处：《山东医药》2017年30期 　论文类型：期刊论文

【摘要】：目的通过蛋白组学研究,鉴定合并2型糖尿病的下肢动脉硬化闭塞症(DLASO)患者与不合并2型糖尿病下肢动脉硬化闭塞症(LASO)患者血清的差异性蛋白,为防治2型糖尿病患者发生LASO提供依据。方法收集DLASO、LASO各15例患者的血清,对蛋白进行提取、定量和酶解,应用同位素标记相对和绝对定量(iTRAQ)结合二维液相色谱串联质谱(2DLC-MS/MS)技术,鉴定DLASO、LASO患者血清中的蛋白,筛选出DLASO患者与LASO患者的差异表达蛋白。利用PANTHER数据库对鉴定得到的差异性蛋白进行生物学过程、分子作用和细胞组分分析。结果共鉴定出蛋白质338种,筛选出差异性蛋白27种,其中DLASO患者出现表达上调蛋白8种、表达下调蛋白19种。在生物学过程方面,差异性蛋白主要为参与细胞反应(48.1%)、代谢过程(48.1%)和生物黏附(29.6%)的蛋白;在分子作用方面,差异性蛋白主要为参与细胞结合作用(33.3%)、催化活性作用(29.6%)和结构分子活性作用(18.5%)的蛋白;在细胞组分方面,差异性蛋白主要为位于细胞区域(14.8%)、细胞器(14.8%)和细胞外区域(14.8%)的蛋白。结论发现DLASO与LASO患者血清中的差异性蛋白27种,这些差异性蛋白可作为防治DLASO的潜在生物学靶点。
[Abstract]:Objective to study on proteomics. To identify the differential proteins in the serum of patients with type 2 diabetes mellitus (DLASO) and patients without type 2 diabetes mellitus (LASO) of lower extremity arteriosclerosis obliterans (LASO). Methods Serum samples of 15 patients with type 2 diabetes mellitus and 15 patients with DLASOLASO were collected to extract, quantify and hydrolyze the protein. The serum proteins of DLASOLASO patients were identified by isotope labeling and absolute quantitative iTRAQ combined with 2D liquid chromatography tandem mass spectrometry (2DLC-MS / MS) technique. The differentially expressed proteins were screened between DLASO patients and LASO patients. The differential proteins were identified by PANTHER database. Results 338 kinds of proteins were identified and 27 kinds of differential proteins were screened, among which 8 kinds of up-regulated proteins were found in patients with DLASO. There are 19 down-regulated proteins. In the biological process, the differential proteins are mainly involved in the cellular reaction (48.1g), the metabolic process (48.1) and the biological adhesion (29.610). In the aspect of molecular action, the differential proteins were mainly involved in cell binding, catalytic activity, catalytic activity and structural molecular activity, respectively. In terms of cell components, the difference protein is mainly located in the cell area of 14.8%. Conclusion there are 27 different proteins in the serum of patients with DLASO and LASO. These differential proteins can be used as potential biological targets for the prevention and treatment of DLASO.
【作者单位】： 南通大学附属医院;复旦大学附属中山医院;
【基金】：上海市科学技术发展基金资助项目(11ZR1406900)
【分类号】：R543.5;R587.1
【正文快照】： 全球范围内近2亿人口患有2型糖尿病,并且其患病率正在快速增加[1]。下肢动脉硬化闭塞症(LASO)是因动脉粥样硬化累及下肢动脉,导致动脉狭窄或闭塞而引起的肢体缺血慢性疾病,可以显著增加心肌梗死、中风等严重疾病的发生率和病死率。合并2型糖尿病时能加速动脉粥样硬化的进展,并，

本文编号：1486792