miR-451a在糖尿病肾病肾纤维化中的临床应用研究

发布时间：2018-02-04 01:58

  本文关键词： miR-a 细胞周期阻滞 纤维化 糖尿病肾病　出处：《现代预防医学》2017年05期 　论文类型：期刊论文

【摘要】：目的研究糖尿病肾病(DN)患者肾组织miR-451a表达量与肾功能、肾纤维化的关系,探讨其临床应用价值。方法对DN患者进行肾活检,活检组织进行病理分级的同时提取RNA通过q PCR分析miR-451a、p15INK4b(p15)、E-caherin、α-SMA的表达变化;提取总蛋白分析各指标蛋白水平变化;并分析尿素、肌酐、尿酸、尿微量白蛋白/肌酐比(ACR)的改变。结果共收集DN患者46例,年龄39~64岁,男28例,女18例。病理分级为I级2例,Ⅱa级6例,Ⅱb级8例,Ⅲ级26例,IV级4例。DN组肾组织miR-451a表达量为对照组的0.2倍(P0.05),p15的mRNA表达量为5.3倍(P0.05)。随纤维化加重,miR-451a、E-caherin水平逐步下降,p15、α-SMA逐步升高(趋势分别为42.21、35.36、31.43、45.96,P0.001)。miR-451a水平与p15、α-SMA、尿素、ACR呈负相关(r=-0.88,-0.83,-0.87,-0.86,P0.05),与E-cadherin呈正相关(r=0.86,P0.05)。P15的蛋白表达量(I级为对照组的2.62倍,Ⅱa级3.00倍,Ⅱb级3.76倍,Ⅲ级3.88倍,IV级4.58倍)、α-SMA的蛋白水平(升至2.24倍,2.82倍,3.00倍,3.10倍,3.64倍),而E-cadherin依次降至0.96,0.61,0.53,0.45,0.29。结论 DN肾纤维化过程中肾miR-451a呈低表达,与周期阻滞、EMT、肾功能存在相关性,可能成为纤维化新的评价指标及治疗靶标。
[Abstract]:Objective to study the relationship between renal miR-451a expression and renal function and renal fibrosis in patients with diabetic nephropathy (DN). RNA was extracted from the biopsy tissues with pathological grading and E-caherin was analyzed by Q PCR analysis of miR-451ap15INK4bP15p15p15cherin. Changes of 伪 -SMA expression; Total protein was extracted to analyze the changes of protein levels. The changes of urea, creatinine, uric acid and urinary albumin / creatinine ratio (ACRs) were analyzed. Results there were 46 DN patients aged 3964 years and 28 males. 18 cases were female. Pathological grading was grade I in 2 cases, grade 鈪，

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