miRNA-467b在EAE中对炎性T细胞浸润的影响

发布时间：2018-02-12 10:27

本文关键词： 实验性自身免疫性脑脊髓膜炎趋化因子受体 miRNA 迁移　出处：《现代免疫学》2017年04期 　论文类型：期刊论文

【摘要】：为探讨miRNA-467b在实验性自身免疫性脑脊髓膜炎(experimental autoimmune encephalomyelitis,EAE)中对炎性T细胞浸润的影响,我们首先采用real-time PCR及FACS方法检测EAE小鼠脾脏单个核细胞中CXCR3、CCR5、CCR2、CCR6的mRNA和蛋白表达水平,并在MOG反应性T细胞中过表达miRNA-467b,FACS方法检测趋化因子受体CXCR3、CCR6的表达变化,并进一步通过Boyden小室(transwell实验)的方法,体外观察miRNA-467b对细胞迁移的影响。结果发现,EAE小鼠脾脏中CCR5、CCR2、CCR6的转录及蛋白表达水平显著上升,在MOG特异性的T细胞中过表达miRNA-467b后,可降低Th1表面趋化因子受体的表达,减少Th1向transwell板下室中的迁移,提示miRNA-467b可以通过影响细胞的迁移进而调节EAE的疾病进程。
[Abstract]:To investigate the effect of miRNA-467b on inflammatory T cell infiltration in experimental autoimmune meningitis autoimmune encephalomytis (EAE), we first detected the expression of mRNA and protein in the spleen mononuclear cells of EAE mice by real-time PCR and FACS. The overexpression of miRNA-467bFACS in MOG reactive T cells was used to detect the expression of chemokine receptor (CXCR3) CCR6, and the expression of CCR6 was detected by Boyden transwell assay. The effect of miRNA-467b on cell migration was observed in vitro. The results showed that the expression of CCR5, CCR2CCR6 and CCR6 protein increased significantly in the spleen of MOG mice. After overexpression of miRNA-467b in MOG specific T cells, the expression of chemokine receptor on Th1 surface was decreased. Reducing the migration of Th1 to the subplate chamber of transwell suggests that miRNA-467b can regulate the progression of EAE by affecting cell migration.
【作者单位】：上海交通大学医学院附属仁济医院输血科;上海交通大学医学院免疫学与微生物学系;上海市免疫学研究所;杭州市余杭区第五人民医院;
【基金】：国家自然科学基金(81373208)
【分类号】：R744.51

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