血清miR-217水平变化与2型糖尿病患者肾损害的关系

发布时间：2018-02-15 12:34

本文关键词： 型糖尿病肾损害微小RNA- 尿白蛋白尿肌酐　出处：《山东医药》2017年21期 　论文类型：期刊论文

【摘要】：目的探讨血清微小RNA-217(miR-217)水平变化与2型糖尿病患者肾损害的关系。方法选择初治2型糖尿病患者490例,根据尿白蛋白与尿肌酐比值(ACR)分为正常白蛋白尿组217例、微量白蛋白尿组171例、大量白蛋白尿组102例;同期选择性别、年龄匹配的体检健康者200例作为对照组。收集各组临床资料并作比较。采用ELISA法检测血清Sirt1、低氧诱导因子1α(HIF-1α)、血管内皮生长因子(VEGF),RT-PCR技术检测血清miR-217。用多元逐步回归分析ACR的影响因素,用Spearman相关分析血清miR-217与其他指标的关系。结果各组血清miR-217、Sirt1、HIF-1α、VEGF水平比较,P均0.05。病程、SUA、BUN、miR-217、Sirt1、HIF-1α、VEGF是ACR的影响因素(β=4.673、3.091、4.105、3.375、-3.250、3.671、3.887,P均0.05)。miR-217与病程、HOMA-IR、Hb A1c、ACR、SUA、BUN、HIF-1α、VEGF呈正相关(rs=0.301、0.394、0.341、0.448、0.381、0.418、0.462、0.475,P均0.05),与Sirt1呈负相关(rs=-0.473,P0.05)。结论高表达的血清miR-217可能通过抑制血清Sirt1表达,调控血清HIF-1α、VEGF水平变化促进2型糖尿病患者肾损害的发生发展。
[Abstract]:Objective to investigate the relationship between serum minimal RNA-217miR-217) level and renal damage in patients with type 2 diabetes mellitus. Methods 490 patients with newly diagnosed type 2 diabetes were divided into normal albuminuria group (217 cases) according to urinary albumin / creatinine ratio (ACRs). 171 cases in microalbuminuria group and 102 cases in large albuminuria group. The clinical data of each group were collected and compared. Serum sirt1, hypoxia inducible factor-1 伪 (HIF-1 伪) and vascular endothelial growth factor (VEGF) were detected by ELISA method and VEGF -217. multivariate stepwise regression was used. The influencing factors of ACR are analyzed. Spearman correlation was used to analyze the relationship between serum miR-217 and other indexes. Results the serum miR-217 sirt1 HIF-1 伪 level was compared with that of HIF-1 伪 in all groups (P < 0.05). In the course of the disease, SUABUNmiR-217HIF-1 伪 was the influential factor of ACR (尾 4.6733.0914.105.37-3.2503.6713.6713.887p, 0.05 .miR-217 and HOMA-IRA1cCRSUAHIF-1 伪). Serum miR-217 may inhibit the expression of serum Sirt1. Regulation of serum HIF-1 伪 -VEGF level promotes the development of renal damage in type 2 diabetic patients.
【作者单位】：遵义医学院附属医院;
【基金】：国家“十二五”科技支撑计划课题(2012BAI10B01)
【分类号】：R587.2;R692.9

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