地塞米松抑制烟曲霉菌暴露的支气管哮喘大鼠肺组织IL-33的表达

发布时间：2018-02-17 03:01

本文关键词： 烟曲霉菌支气管哮喘白细胞介素地塞米松 Wistar大鼠　出处：《复旦学报(医学版)》2017年03期 　论文类型：期刊论文

【摘要】：目的研究烟曲霉菌暴露对支气管哮喘大鼠肺组织IL-33水平的影响及地塞米松的作用。方法将24只Wistar大鼠随机分为A组(正常对照组)、B组(哮喘组)、C组(烟曲霉菌暴露组)、D组(地塞米松干预组),每组6只。B、C、D组均以卵白蛋白(ovalbumin,OVA)致敏、激发的方法构建哮喘模型;C、D组在哮喘模型构建成功后,给予烟曲霉菌孢子鼻腔滴入;D组在OVA激发阶段给予地塞米松干预;A组则予等量生理盐水致敏、激发、滴鼻作为对照。通过气道高反应性检测、嗜酸性粒细胞百分比及血清IgE水平确定哮喘模型的建立,ELISA法及qRT-PCR法检测肺组织IL-33的表达,大鼠肺组织和全血于马铃薯葡萄糖琼脂培养基(potato dextrose agar,PDA)上培养24 h后观察烟曲霉菌菌落生长情况。结果 B、C组与A组比较,肺泡灌洗液中嗜酸性粒细胞百分比、血清IgE水平及肺组织IL-33的表达均明显升高(P0.05),且C组比B组升高更明显。各组间气道反应性指标sRaw变化值差异不明显。D组与C组比较,嗜酸性粒细胞百分比及肺组织IL-33的表达均明显降低(P0.05),血清IgE水平降低不显著,但肺组织烟曲霉菌培养阳性率高于C组。结论糖皮质激素(地塞米松)能够抑制烟曲霉菌暴露的支气管哮喘大鼠肺组织IL-33的表达,但增加了气道真菌定植的风险,针对IL-33的阻断治疗有待进一步研究。
[Abstract]:Objective to study the effect of Aspergillus fumigatus exposure on IL-33 level in lung tissue and the effect of dexamethasone on lung tissue in asthmatic rats. Methods 24 Wistar rats were randomly divided into group A (normal control group) and group B (Asthma group) (Aspergillus fumigatus group). Group D was sensitized with ovalbumin OVA in group D (dexamethasone intervention group, 6 rats in each group). Methods the asthmatic model was established. After the successful establishment of the asthma model, Aspergillus fumigatus was given nasal cavity drip into the nasal cavity of Aspergillus fumigatus, and dexamethasone was given to treat the asthma model in the OVA stimulation stage. Group A was sensitized and stimulated by the same amount of normal saline. The airway hyperresponsiveness, the percentage of eosinophil and the level of serum IgE were used to determine the expression of IL-33 in lung tissue of asthmatic model by Elisa and qRT-PCR. Lung tissue and whole blood of rats were cultured on potato glucose Agar medium for 24 hours to observe the growth of Aspergillus fumigatus colony. Results the percentage of eosinophils in alveolar lavage fluid was higher in group C than in group A. The level of serum IgE and the expression of IL-33 in lung tissue were significantly higher in group C than in group B. the changes of sRaw in group D were not significantly different from those in group C. there was no significant difference between group D and group C. The percentage of eosinophilic granulocytes and the expression of IL-33 in lung tissue were significantly decreased, but the serum IgE level was not significantly decreased. Conclusion glucocorticoid (dexamethasone) can inhibit the expression of IL-33 in lung tissue of Aspergillus fumigatus exposed asthma rats, but increase the risk of airway fungal colonization. The blocking therapy for IL-33 needs further study.
【作者单位】：复旦大学附属华山医院呼吸科;
【基金】：上海市科委基础研究领域重点项目(12JC1407501)~~
【分类号】：R562.25

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