颈交感神经干离断对2型糖尿病大鼠围术期T淋巴细胞亚群及IL-2和IL-6的影响

发布时间：2018-02-22 12:47

本文关键词： 颈交感神经干离断型糖尿病围术期 T淋巴细胞亚群　出处：《广东医学》2017年14期 　论文类型：期刊论文

【摘要】：目的探讨颈交感神经干离断(TCST)对2型糖尿病大鼠围术期T淋巴细胞亚群及白细胞介素(IL)-2、IL-6的影响。方法清洁级健康成年雄性SD大鼠,10~12周龄,体重250~280 g,采用高脂高糖饮食复合腹腔注射链脲佐菌素(STZ)制备2型糖尿病大鼠模型。取模型制备成功的大鼠90只,采用随机数字表法分为3组:糖尿病组(D组)、糖尿病~+剖腹探查组(DO组)和糖尿病~+TCST~+剖腹探查组(DTO组),每组30例。于糖尿病模型制备成功1周后,DTO组行右侧TCST术制备TCST模型,D组和DO组仅分离右颈交感神经干,不离断。DO组和DTO组于TCST模型制备成功1周后行剖腹探查术,分别于切皮前(T0)、术毕(T1)、术后第1天(T2)、术后第3天(T3)和术后第7天(T4)随机取6只大鼠,测定3组大鼠尾静脉血糖,采集血样后处死,采用ELISA法检测血浆去甲肾上腺素(NE)、IL-2和IL-6浓度,流式细胞术测定CD3~+、CD4~+、CD8~+T细胞百分比,并计算CD4~+/CD8~+比值。结果与D组比较,DO组T1~T4时血糖、血浆NE和IL-6浓度明显升高,CD3~+、CD4~+T细胞百分比、CD4~+/CD8~+比值和IL-2浓度明显降低(P0.05);与DO组比较,DTO组T1~T4时血糖、血浆NE和IL-6浓度明显降低,CD3~+、CD4~+T细胞百分比、CD4~+/CD8~+比值和血浆IL-2浓度明显升高(P0.05)。结论 TCST可升高2型糖尿病大鼠围术期CD3~+、CD4~+T细胞百分比、CD4~+/CD8~+比值和血浆IL-2浓度,有助于改善2型糖尿病大鼠围术期细胞免疫功能。
[Abstract]:Objective to investigate the effect of TCST on perioperative T lymphocyte subsets and interleukin-2 interleukin-6 (IL-6) in type 2 diabetic rats. Methods healthy adult male SD rats of clean grade were 10 ~ 12 weeks old. The model of type 2 diabetic rats was established by high fat and high sugar diet and intraperitoneal injection of streptozotocin (STZ). Three groups were randomly divided into three groups: diabetic group D group, diabetes group D group and diabetic TCST-laparotomy group (30 cases in each group). One week after the diabetic model was made, the right TCST was performed in the DTO group. The right cervical sympathetic trunk was isolated from the right cervical sympathetic trunk in group D and group do. In the do group and DTO group, the caesarean section was performed 1 week after the TCST model was made. The rats in the three groups were randomly selected to measure the blood glucose in the caudal vein of the 3 groups, which were treated with T0, T1, T2, T3 and T4 on the 1st day and 7th day after the operation, respectively, before the incision of the skin, and at the end of the operation, the rats were divided into two groups, one day after the operation, one day after the operation, and one day after the operation, the caudal vein blood glucose was measured. Blood samples were collected and killed. The concentrations of IL-2 and IL-6 were detected by ELISA method, the percentage of CD3 ~ + CD4 ~ + CD8 ~ T cells was measured by flow cytometry, and the ratio of CD4 ~ / CD8 ~ ~ was calculated. Plasma NE and IL-6 concentrations significantly increased the percentage of CD4 ~ / CD4T cells and the concentration of IL-2, and significantly decreased the blood glucose levels of T1 / T4 and T1 / T4 in the do group, compared with those in the do group, and the ratio of CD4 ~ / / CD8 ~ + and the concentration of IL-2 were significantly decreased in the plasma, compared with those in the do group. Plasma NE and IL-6 decreased significantly the percentage of CD4 ~ / / CD8 ~ T cells and the concentration of IL-2 in plasma. Conclusion TCST can increase the percentage of CD3 ~ + CD4- T cells and the concentration of plasma IL-2 in type 2 diabetic rats during perioperative period. It is helpful to improve the cellular immune function in type 2 diabetic rats during perioperative period.
【作者单位】：南昌大学第二附属医院麻醉科;德安县中医院麻醉科;上高县人民医院麻醉科;
【基金】：国家自然科学基金资助项目(编号:81560193) 江西省卫生计生委科技计划项目(编号:20161076)
【分类号】：R587.1;R614

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