聚乳酸共聚物复合脂肪干细胞对骨质疏松性骨折愈后生物力学的影响

发布时间：2018-02-22 21:29

  本文关键词： 骨质疏松性骨折 干细胞 生物力学 组织工程 生物材料 骨生物材料 聚乳酸共聚物 骨质疏松骨折 大鼠脂肪干细胞 生物力学参数　出处：《中国组织工程研究》2017年10期 　论文类型：期刊论文

【摘要】：背景:聚乳酸共聚物骨支架具有优异的生物降解性且易被塑造为三维立体支架,能促进骨组织和血管的生成、生长。目的:观察骨质疏松骨折后,聚乳酸共聚物复合脂肪干细胞对骨折愈合后生物力学的影响。方法:将60只SD大鼠随机分为4组,空白对照组不做任何处理;模型组切除双侧卵巢3个月后,制作双侧胫骨骨折模型;细胞治疗组切除双侧卵巢3个月后,制作双侧胫骨骨折模型,于骨折周围植入脂肪干细胞;联合治疗组切除双侧卵巢3个月后,制作双侧胫骨骨折模型,于骨折周围植入聚乳酸共聚物-脂肪干细胞复合体。治疗4周后,观察各组骨密度、骨痂厚度、生物力学参数及骨小梁微结构变化。结果与结论:(1)骨密度:模型组骨密度低于空白对照组(P0.05);细胞治疗组、联合治疗组骨密度高于模型组(P0.05),但低于空白对照组(P0.05);联合治疗组骨密度高于细胞治疗组(P0.05);(2)骨痂厚度:细胞治疗组、联合治疗组骨痂厚度高于模型组、空白对照组(P0.05),且联合治疗组高于细胞治疗组(P0.05);(3)生物力学测试:与空白对照组比较,模型组破坏载荷、极限应力、剪切强度、弹性模量降低(P0.05),剪切应变升高(P0.05);与模型组比较,细胞治疗组、联合治疗组破坏载荷、极限应力、剪切强度、弹性模量升高(P0.05),剪切应变降低(P0.05),且以联合治疗组改变更显著(P0.05);(4)骨小梁微结构:模型组骨小梁排列紊乱,间距增大,甚至出现断裂和陷窝;细胞治疗组、联合治疗组骨小梁数目增多且增粗增厚,间距减小,陷窝减少;(5)结果表明:聚乳酸共聚物复合脂肪干细胞治疗骨质疏松骨折可明显改善愈合后骨组织的生物力学参数。
[Abstract]:Background: Polylactic acid copolymer bone scaffolds have excellent biodegradability and are easily molded as three-dimensional scaffolds, which can promote the formation and growth of bone tissue and blood vessels. Effects of Polylactic Acid Copolymer combined with adipose Stem cells on Biomechanics after fracture Healing methods: 60 SD rats were randomly divided into 4 groups without any treatment in the blank control group after 3 months of bilateral ovary resection in the model group. The model of bilateral tibial fracture was made; the model of bilateral tibial fracture was made after 3 months of bilateral ovarian resection in the cell therapy group, and adipose stem cells were implanted around the fracture; in the combined treatment group, the bilateral ovary was removed for 3 months. A bilateral tibial fracture model was established and the poly (lactic acid) copolymer-adipose stem cell complex was implanted around the fracture. After 4 weeks of treatment, bone mineral density (BMD) and callus thickness were observed in each group. Biomechanical parameters and bone trabecular microstructure.Results and conclusion: BMD in the model group was lower than that in the blank control group (P 0.05), and in the cell therapy group, the bone density in the model group was lower than that in the control group. The bone mineral density of the combined treatment group was higher than that of the model group, but lower than that of the blank control group (P 0.05); the bone mineral density of the combined treatment group was higher than that of the cell therapy group (P 0.05); the callus thickness of the combined treatment group was higher than that of the model group, and the callus thickness of the combined treatment group was higher than that of the model group. Compared with the blank control group, the damage load, ultimate stress, shear strength and elastic modulus of the model group decreased by P0.05A, and the shear strain increased by P0.05and compared with the model group. In the combined treatment group, the damage load, ultimate stress, shear strength and modulus of elasticity increased (P 0.05), shear strain decreased (P 0.05), and the bone trabecular structure was changed more significantly in the combined treatment group: the trabeculae in the model group were disordered, and the spacing was increased. In the cell therapy group, the number of trabeculae in the combined treatment group increased, the bone trabeculae became thicker and thicker, and the distance between the bone trabeculae decreased. The results showed that the biomechanical parameters of bone tissue after healing could be significantly improved by the treatment of osteoporotic fracture with poly (lactic acid) copolymer combined with adipose stem cells.
【作者单位】： 中山大学附属第五医院骨一科;
【基金】：广东省医学科研基金立项课题(2014A030313737)~~
【分类号】：R580;R683
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